I'm currently trying to use readFastq with the ShortRead library. I have specified the version of Bioconductor I need (3.14) in order to run it in R version 4.1 (which is the one I'm currently using). I keep getting an error message saying:
> library(ShortRead)
Error in library(Shortread) : there is no package called 'ShortRead'
and also:
ln: failed to create symbolic link 'libhts.so.2': Operation not supported
make: *** [Makevars:28: populate-usrlib-dir] Error 1
ERROR: compilation failed for package ‘Rhtslib’
* removing ‘/home/jovyan/work/__dag_config__/R/libs/Rhtslib’
ERROR: dependency ‘Rhtslib’ is not available for package ‘Rsamtools’
* removing ‘/home/jovyan/work/__dag_config__/R/libs/Rsamtools’
ERROR: dependency ‘Rsamtools’ is not available for package ‘GenomicAlignments’
* removing ‘/home/jovyan/work/__dag_config__/R/libs/GenomicAlignments’
ERROR: dependencies ‘Rsamtools’, ‘GenomicAlignments’, ‘Rhtslib’ are not available for package ‘ShortRead’
* removing ‘/home/jovyan/work/__dag_config__/R/libs/ShortRead’
and
ERROR: configuration failed for package ‘textshaping’
* removing ‘/opt/conda/envs/r/lib/R/library/textshaping’
* restoring previous ‘/opt/conda/envs/r/lib/R/library/textshaping’
* installing *source* package ‘ragg’ ...
** package ‘ragg’ successfully unpacked and MD5 sums checked
** using staged installation
and lastly
ERROR: configuration failed for package ‘ragg’
* removing ‘/opt/conda/envs/r/lib/R/library/ragg’
* restoring previous ‘/opt/conda/envs/r/lib/R/library/ragg’
The downloaded source packages are in
‘/tmp/RtmpgByRyT/downloaded_packages’
I would really appreciate som help with troubleshooting this or a "smarter" way to convert fastq.gz files to fasta files
The commands I've run so far are:
#Installing ShortRead package containing the readFastq function
if (!require("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install(version="3.14")
BiocManager::install("GenomeInfoDbData")
BiocManager::install("GenomeInfoDb")
BiocManager::install("ShortRead")
#Loading the library
library(ShortRead)
Some extra session information:
> sessionInfo()
R version 4.1.1 (2021-08-10)
Platform: x86_64-conda-linux-gnu (64-bit)
Running under: Ubuntu 22.04.1 LTS
Matrix products: default
BLAS/LAPACK: /opt/conda/envs/r/lib/libopenblasp-r0.3.21.so
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=en_US.UTF-8
[4] LC_COLLATE=en_US.UTF-8 LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C LC_ADDRESS=C
[10] LC_TELEPHONE=C LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] BiocManager_1.30.19
loaded via a namespace (and not attached):
[1] compiler_4.1.1 fastmap_1.1.0 cli_3.4.1 htmltools_0.5.3 tools_4.1.1
[6] rstudioapi_0.14 yaml_2.3.6 rmarkdown_2.17 knitr_1.40 xfun_0.34
[11] digest_0.6.30 rlang_1.0.6 evaluate_0.17
I do not understand why it won't install. I would it expect it to work when I have made sure to use the correct version
Related
I am pretty desperate to install.packages("devtools").
However it allways fails with this error
Error: .onLoad failed in loadNamespace() for 'pkgload', details:
call: readRDS(nsInfoFilePath)
error: error reading from connection
Execution halted
ERROR: lazy loading failed for package ‘devtools’
* removing ‘/home/bjoern/R/x86_64-pc-linux-gnu-library/3.6/devtools’
Things I tried to fix this:
Add the dependencies = T argument
Following a suggestion by hadley wickham on github -> update rlang package
Try to run install.packages("pkgload") resulting in nearly exactly the same error (see below)
Completely removing R and reinstalling it
Additionally installing package following packages:
r-base-dev
r-cran-devtools
r-recommended
Updated all packages update.packages(ask = FALSE, checkBuilt = TRUE)
Error of install.packages("pkgload")
Error: package or namespace load failed for ‘pkgload’:
.onLoad failed in loadNamespace() for 'pkgload', details:
call: readRDS(nsInfoFilePath)
error: error reading from connection
Error: loading failed
Execution halted
ERROR: loading failed
I digged even deeper to the pkgload (which at least I hope is the only reason for the problem). I will check if manually (re)installing all imports and suggests from the CRAN page of pkgload does solve it.
Imports: desc, methods, pkgbuild, rlang, rprojroot, rstudioapi,
utils, withr Suggests: bitops, covr, Rcpp, testthat
My sessionInfo() output:
R version 3.6.3 (2020-02-29)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 20.04 LTS
Matrix products: default
BLAS: /usr/lib/x86_64-linux-gnu/blas/libblas.so.3.9.0
LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.9.0
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=de_DE.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=de_DE.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=de_DE.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=de_DE.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
loaded via a namespace (and not attached):
[1] compiler_3.6.3 tools_3.6.3
I still refuse to belief that it is impossibe to install devtools on ubuntu20
However at this point I take any straw
Solution Edit:
In short what fixed the issue for me was installing testthat package before. Thanks to comment by rui-barradas
install.packages("testthat")
install.packages("pkgload")
install.packages("devtools")
I am also on Ubuntu 20.04 but running R 4.0.0.
When trying to install a package, if it depends on another that was installed prior to R 4.0.0 I get an error message and the installation fails. This happens even if I set dependencies = TRUE in the call to install.packages.
Example: The command was
install.packages('pkgload')
I omit the first output lines, saying that the file was downloaded. Then, the relevant part.
installing source package ‘pkgload’ ...
** package ‘rcmdcheck’ successfully unpacked and MD5 sums checked
** using staged installation
** R
** byte-compile and prepare package for lazy loading Error: package ‘testthat’ was installed before R 4.0.0: please re-install it
Execution halted ERROR: lazy loading failed for package ‘pkgload’
removing ‘/usr/local/lib/R/site-library/pkgload’
restoring previous ‘/usr/local/lib/R/site-library/pkgload’ Warning in install.packages : installation of package ‘pkgload’ had
non-zero exit status The downloaded source packages are in
‘/tmp/RtmpVxpbs0/downloaded_packages’
Correct way.
The right way of installing package pkgload was to run the sequence
install.packages('testthat')
install.packages('pkgload')
and the problem was solved.
Note that with other packages, it already happened to see this be a recursive issue. Imagine that package testthat depended (which it does not) on another package installed prior to R 4.0.0.
Annoying but solvable.
sessionInfo()
#R version 4.0.0 (2020-04-24)
#Platform: x86_64-pc-linux-gnu (64-bit)
#Running under: Ubuntu 20.04 LTS
#
#Matrix products: default
#BLAS: /usr/lib/x86_64-linux-gnu/blas/libblas.so.3.9.0
#LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.9.0
#
#locale:
# [1] LC_CTYPE=pt_PT.UTF-8 LC_NUMERIC=C
#[3] LC_TIME=pt_PT.UTF-8 LC_COLLATE=pt_PT.UTF-8
# [5] LC_MONETARY=pt_PT.UTF-8 LC_MESSAGES=pt_PT.UTF-8
# [7] LC_PAPER=pt_PT.UTF-8 LC_NAME=C
# [9] LC_ADDRESS=C LC_TELEPHONE=C
#[11] LC_MEASUREMENT=pt_PT.UTF-8 LC_IDENTIFICATION=C
#
#attached base packages:
#[1] stats graphics grDevices utils datasets
#[6] methods base
#
#other attached packages:
#[1] rvest_0.3.5 xml2_1.3.2 data.table_1.12.8
#
#loaded via a namespace (and not attached):
I had the same problems, and the solution was, at least in my case, Ubuntu 20.04.1 LTS (Focal Fossa) + R 4.0.2:
First (from terminal), install:
Step 1: sudo apt-get update -y
Step 2: sudo apt-get install -y libxml2-dev
Then from RStudio (setRepositories first, and choose 8 repositories) and install both packages:
Install xml2 package
install devtools
And works for me.
Every package I try to install is met with the following error:
* installing *source* package ‘dichromat’ ...
** package ‘dichromat’ successfully unpacked and MD5 sums checked
** R
** data
** preparing package for lazy loading
** help
*** installing help indices
converting help for package ‘dichromat’
finding HTML links ... done
colorschemes html
dalton html
Error: invalid regular expression '^[[:blank:]]*
'
* removing ‘/newhome/vdp5/R/x86_64-redhat-linux-gnu-library/3.3/dichromat’
ERROR: dependency ‘dichromat’ is not available for package ‘scales’
* removing ‘/newhome/vdp5/R/x86_64-redhat-linux-gnu-library/3.3/scales’
The downloaded source packages are in
‘/tmp/RtmpVXl3tg/downloaded_packages’
Warning messages:
1: In install.packages("scales") :
installation of package ‘dichromat’ had non-zero exit status
2: In install.packages("scales") :
installation of package ‘scales’ had non-zero exit status
I have no idea why this installation keeps failing and have been unable to make any progress in potentially hacking together a solution.
My session info is as follows:
R version 3.3.2 (2016-10-31)
Platform: x86_64-redhat-linux-gnu (64-bit)
Running under: Red Hat Enterprise Linux
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
I'm running R v3.2.0 with RStudio as well. The machine I'm running on has no internet access and therefore I'm having to download and install packages locally. I am trying to install ggplot2 which requires lots of dependecies (with their own dependencies) to be downloaded and installed. I have arrived at the point where most of them have been installed. However, I still get the following when I try:
> install.packages("~/R_packages/ggplot2_2.0.0.tar.gz", repos = NULL, type = "source")
Installing package into ‘/u01/app/oracle/product/12.1.0.2/dbhome_1/R/library’
(as ‘lib’ is unspecified)
* installing *source* package ‘ggplot2’ ...
** package ‘ggplot2’ successfully unpacked and MD5 sums checked
** R
** data
*** moving datasets to lazyload DB
** inst
** preparing package for lazy loading
No protocol specified
Warning in fun(libname, pkgname) : couldn't connect to display ":0"
Error in dyn.load(file, DLLpath = DLLpath, ...) :
unable to load shared object '/u01/app/oracle/product/12.1.0.2/dbhome_1/R/library/scales/libs/scales.so':
/u01/app/oracle/product/12.1.0.2/dbhome_1/R/library/scales/libs/scales.so: invalid ELF header
ERROR: lazy loading failed for package ‘ggplot2’
* removing ‘/u01/app/oracle/product/12.1.0.2/dbhome_1/R/library/ggplot2’
Warning in install.packages :
installation of package ‘/home/oracle/R_packages/ggplot2_2.0.0.tar.gz’ had non-zero exit status
> library(ggplot2)
Error in library(ggplot2) : there is no package called ‘ggplot2’
Has anyone got any ideas why this is happening?
Edit:
Oracle Distribution of R version 3.2.0 (--)
Platform: x86_64-unknown-linux-gnu (64-bit)
Running under: Oracle Linux Server 6.7
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=en_US.UTF-8
[4] LC_COLLATE=en_US.UTF-8 LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=en_US.UTF-8 LC_ADDRESS=en_US.UTF-8
[10] LC_TELEPHONE=en_US.UTF-8 LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=en_US.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] dplyr_0.4.2 stringi_0.4-1 nnet_7.3-9 randomForest_4.6-10 ROracle_1.2-1
[6] DBI_0.3.1 rpart_4.1-9 ORCH_2.5.1 ORCHstats_2.5.1 ORCHcore_2.5.1
[11] rJava_0.9-7 OREstats_1.5 MASS_7.3-40 OREbase_1.5 OREcommon_1.5
loaded via a namespace (and not attached):
[1] Rcpp_0.12.2 png_0.1-7 assertthat_0.1 R6_2.0.1 grid_3.2.0 OREembed_1.5 magrittr_1.5
[8] tools_3.2.0 parallel_3.2.0
Edit 2:
library(ggplot2)
No protocol specified
Attaching package: ‘ggplot2’
The following object is masked from ‘package:randomForest’:
margin
Warning message:
In fun(libname, pkgname) : couldn't connect to display ":0"
I have just initiated a EC2 server on AWS and I'm trying to install a package from
github called 'krose/skm'. The package is installed without any issues on Windows, but not on Ubuntu. All dependencies are installed. I can't figure out why it can install on Windows, but not Ubuntu.
library(devtools)
install_github('krose/skm')
And the result of running the code above is:
Downloading GitHub repo krose/skm#master
from URL https://api.github.com/repos/krose/skm/zipball/master
Installing skm
'/usr/lib/R/bin/R' --no-site-file --no-environ --no-save --no-restore --quiet \
CMD INSTALL '/tmp/RtmpxJg8N7/devtools1b887e511e96/krose-skm-059766b' \
--library='/usr/local/lib/R/site-library' --install-tests
* installing *source* package ‘skm’ ...
** help
*** installing help indices
** building package indices
** testing if installed package can be loaded
Error in namespaceExport(ns, exports) :
undefined exports: skm_umm_stations, skm_ummquery, skm_webquery
Error: loading failed
Execution halted
ERROR: loading failed
* removing ‘/usr/local/lib/R/site-library/skm’
Error: Command failed (1)
The functions are documented using Roxygen2 and are exported. The functions are not assigned using <<-.
Here is my sessionInfo()
R version 3.2.5 (2016-04-14)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 14.04.4 LTS
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] devtools_1.11.1
loaded via a namespace (and not attached):
[1] httr_1.1.0 R6_2.1.2 tools_3.2.5 withr_1.0.1 curl_0.9.7
[6] memoise_1.0.0 git2r_0.14.0 digest_0.6.9
I don't have much experience with Ubuntu.
UPDATE: The R CMD check has the following two notes:
* checking DESCRIPTION meta-information ... NOTE
License components which are templates and need '+ file LICENSE':
MIT
* checking top-level files ... NOTE
Non-standard file/directory found at top level:
'README.Rmd'
The R directory in the package was misnamed as r. Windows didn't pick up on the difference, since it is more forgiving about differences in case for file and directory names.
Trying to install package "caret" on R 3.1.0 (Ubuntu 14.04.01 LTS) and running into a build error.
> install.packages('caret')
[...]
* installing *source* package ‘BradleyTerry2’ ...
** package ‘BradleyTerry2’ successfully unpacked and MD5 sums checked
** R
** data
*** moving datasets to lazyload DB
** inst
** preparing package for lazy loading
Error : object ‘brglm’ is not exported by 'namespace:brglm'
ERROR: lazy loading failed for package ‘BradleyTerry2’
* removing ‘/home/br00t/R/x86_64-pc-linux-gnu-library/3.1/BradleyTerry2’
Warning in install.packages :
installation of package ‘BradleyTerry2’ had non-zero exit status
ERROR: dependency ‘BradleyTerry2’ is not available for package ‘caret’
* removing ‘/home/br00t/R/x86_64-pc-linux-gnu-library/3.1/caret’
Warning in install.packages :
installation of package ‘caret’ had non-zero exit status
The downloaded source packages are in
‘/tmp/Rtmp7pWmvr/downloaded_packages’
> sessionInfo()
R version 3.1.0 (2014-04-10)
Platform: x86_64-pc-linux-gnu (64-bit)
locale:
[1] LC_CTYPE=en_CA.UTF-8 LC_NUMERIC=C LC_TIME=en_CA.UTF-8 LC_COLLATE=en_CA.UTF-8
[5] LC_MONETARY=en_CA.UTF-8 LC_MESSAGES=en_CA.UTF-8 LC_PAPER=en_CA.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C LC_MEASUREMENT=en_CA.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] sp_1.0-16
loaded via a namespace (and not attached):
[1] grid_3.1.0 lattice_0.20-29 tools_3.1.0
Wasn't able to find anything helpful on the interwebs. Not sure if this is an issue with the BradleyTerry2 package or brglm... help?
Thanks,
br00t
If you are running a version of the brglm package greater than 0.5-9, uninstall and install brglm package version 0.5-9
I was able to get over the issue too by installing the dependencies. You may want to select your answer as the right one.