signalr/negotiate 403 - forbidden: access is denied - signalr

Any one help me this:
On localhost this working, but on IIS, win server 2019 i get signalr/negotiate 403 - forbidden: access is denied.
Note that I use MVC 5 asp.net, not asp.net core
On file Startup, I add app.MapSignalR();
[enter image description here][1]
[1]: https://i.stack.imgur.com/WxOkU.jpg
Thank you for your help!

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