Remove vertex.tooltips from ndtv d3movie - r

I'm plotting a dynamic network visulisation using the Statnet, networkd3, and ndtv packages.
In the vignette for this package, the authors explain how to "generate interactive html tooltips to be displayed when the vertices are clicked on":
render.d3movie(
toy_epi_sim,
render.par = render.par,
plot.par = plot.par,
vertex.cex = 0.9,
vertex.col = "ndtvcol",
edge.col = "darkgrey",
vertex.border = "lightgrey",
displaylabels = FALSE,
vertex.tooltip = function(slice){paste('name:',slice%v%'vertex.names','<br>',
'status:', slice%v%'testatus')},
output.mode='htmlWidget')
However, I'd like to remove these tooltips, since by default they just show meaningless node IDs, and I'm already showing node labels instead.
Things like vertex.tooltip = F or vertex.tooltip = NA don't work - how do I turn off vertex tooltips?
This is the code I'm using:
render.d3movie(net, usearrows = T, label = "gmv_label",
displaylabels = T,
bg="#ffffff",
edge.col = "#ff0063",
vertex.col = "colo",
vertex.cex = "amount",
edge.lwd = 5,
launchBrowser=T, filename= XXX,
render.par=list(tween.frames = 30, show.time = F),
plot.par=list(mar=c(0,0,0,0)))

Related

How to use a newline in node label - forceNetwork R Shiny

I am trying to include the ability to have newlines in a node label, otherwise they extend out pretty far and just look messy in my R Shiny application. How can I do this? I have tried including the html break character and newline character but neither has worked. My network is created using the following code
forceNetwork(
Links = edges,
Nodes = nodes,
fontFamily = "Arial",
Nodesize = "size",
Source = "source_id",
Target = "target_id",
NodeID = "type_2",
Group = "type",
zoom = TRUE,
opacity = 1,
charge = -100,
linkColour = color_string_link,
opacityNoHover = ifelse(input$togglelabels == "labels",1,0),
colourScale = JS(color_string)
)

Heatmap of Gene intensity values in R

I have data that look like this:
Gene
HBEC-KT-01
HBEC-KT-02
HBEC-KT-03
HBEC-KT-04
HBEC-KT-05
Primarycells-02
Primarycells-03
Primarycells-04
Primarycells-05
BPIFB1
15726000000
15294000000
15294000000
14741000000
22427000000
87308000000
2.00E+11
1.04E+11
1.51E+11
LCN2
18040000000
26444000000
28869000000
30337000000
10966000000
62388000000
54007000000
56797000000
38414000000
C3
2.52E+11
2.26E+11
1.80E+11
1.80E+11
1.78E+11
46480000000
1.16E+11
69398000000
78766000000
MUC5AC
15647000
8353200
12617000
12221000
29908000
40893000000
79830000000
28130000000
69147000000
MUC5B
965190000
693910000
779970000
716110000
1479700000
38979000000
90175000000
41764000000
50535000000
ANXA2
14705000000
18721000000
21592000000
18904000000
22657000000
28163000000
24282000000
21708000000
16528000000
I want to make a heatmap like the following using R. I am following a paper and they quoted "Heat maps were generated with the ‘pheatmap’ package76, where correlation clustering distance row was applied". Here is their heatmap.
I want the same like this and I am trying to make one using R by following tutorials but I am new to R language and know nothing about R.
Here is my code.
df <- read.delim("R.txt", header=T, row.names="Gene")
df_matrix <- data.matrix(df)
pheatmap(df_matrix,
main = "Heatmap of Extracellular Genes",
color = colorRampPalette(rev(brewer.pal(n = 10, name = "RdYlBu")))(10),
cluster_cols = FALSE,
show_rownames = F,
fontsize_col = 10,
cellwidth = 40,
)
This is what I get.
When I try using clustering, I got the error.
pheatmap(
mat = df_matrix,
scale = "row",
cluster_column = F,
show_rownames = TRUE,
drop_levels = TRUE,
fontsize = 5,
clustering_method = "complete",
main = "Hierachical Cluster Analysis"
)
Error in hclust(d, method = method) :
NA/NaN/Inf in foreign function call (arg 10)
Can someone help me with the code?
You can normalize the data using scale to archive a more uniform coloring. Here, the mean expression is set to 0 for each sample. Genes lower expressed than average have a negative z score:
library(tidyverse)
library(pheatmap)
data <- tribble(
~Gene, ~`HBEC-KT-01`, ~`HBEC-KT-02`, ~`HBEC-KT-03`, ~`HBEC-KT-04`, ~`HBEC-KT-05`, ~`Primarycells-03`, ~`Primarycells-04`, ~`Primarycells-05`,
"BPIFB1", 1.5726e+10, 1.5294e+10, 1.5294e+10, 1.4741e+10, 2.2427e+10, 2e+11, 1.04e+11, 1.51e+11,
"LCN2", 1.804e+10, 2.6444e+10, 2.8869e+10, 3.0337e+10, 1.0966e+10, 5.4007e+10, 5.6797e+10, 3.8414e+10,
"C3", 2.52e+11, 2.26e+11, 1.8e+11, 1.8e+11, 1.78e+11, 1.16e+11, 6.9398e+10, 7.8766e+10,
"MUC5AC", 15647000, 8353200, 12617000, 12221000, 29908000, 7.983e+10, 2.813e+10, 6.9147e+10,
"MUC5B", 965190000, 693910000, 779970000, 716110000, 1479700000, 9.0175e+10, 4.1764e+10, 5.0535e+10,
"ANXA2", 1.4705e+10, 1.8721e+10, 2.1592e+10, 1.8904e+10, 2.2657e+10, 2.4282e+10, 2.1708e+10, 1.6528e+10
)
data %>%
mutate(across(where(is.numeric), scale)) %>%
column_to_rownames("Gene") %>%
pheatmap(
scale = "row",
cluster_column = F,
show_rownames = FALSE,
show_colnames = TRUE,
treeheight_col = 0,
drop_levels = TRUE,
fontsize = 5,
clustering_method = "complete",
main = "Hierachical Cluster Analysis (z-score)",
)
Created on 2021-09-26 by the reprex package (v2.0.1)

How to save simpleNetwork output from networkD3 in PDF/JPEG/TIFF format?

I am new to Rstudio and have just plotted a network diagram using simpleNetwork in the networkD3 package. I am looking to save the output in PDF/JPEG/TIFF format for my PhD thesis, however, most suggestions are with respect to saving it on a website (.html).
My code is as follows:
simpleNetwork(data, Source = 1, Target = 2, height = NULL, width = NULL,
linkDistance = 120, charge = -40, fontSize = 12, fontFamily = "serif",
linkColour = "grey", nodeColour = "black", opacity = 1.0, zoom = F)
Everything runs successfully, however I am not able to save the output as a PDF/JPEG/TIFF.
Is there any way I could save it in a picture or pdf format?
Here is what I have done in the past using the 'htmlwidgets' and 'webshot' packages:
g <- simpleNetwork(data, Source = 1, Target = 2, height = NULL, width = NULL,
linkDistance = 120, charge = -40, fontSize = 12, fontFamily = "serif",
linkColour = "grey", nodeColour = "black", opacity = 1.0, zoom = F)
require(htmlwidgets)
saveWidget(g, file="name_of_your_file.html")
require(webshot)
webshot("file:///C:/Users/.../name_of_your_file.html", "name_of_your_pdf.pdf")
sometimes, a picture is worth a thousand words...

'use.edge.length = FALSE' doesn't seem to work when using plotBranchbyTrait() in phytools

I'm trying to create a phylogeny where the branch lengths that I've coded are represented by colour rather than length. So I want the branch lengths to be equal.
Here is my code:
plotBranchbyTrait(tree.scaled, tree.scaled$edge.length, mode=c("edges"),palette="rainbow", use.edge.length = FALSE, node.depth = 2)
It's my understanding that use.edge.length = FALSE should make the branch lengths equal, and it does this if I code the tree using plot.phylo(). But the tree still shows up with the branch lengths when I use plotBranchbyTrait(). Anyone know how to get around this?
Unfortunately, optional arguments (...) are not directly passed to plot.phylo in the plotBranchbyTrait function. One non-elegant way to fix that is to modify the body directly in R to add a hard coded use.edge.length = FALSE option.
You can do this by creating a new function and modify it using body(foo)[[line_of_interest]] <- substitute(my_new_line <- that_does_something). The following example should work:
## Back up the function
plotBranchbyTrait_no_edge_length <- phytools::plotBranchbyTrait
## The line to modify:
body(plotBranchbyTrait_no_edge_length)[[34]]
# xx <- plot.phylo(tree, type = type, show.tip.label = show.tip.label,
# show.node.label = show.node.label, edge.color = colors, edge.width = edge.width,
# edge.lty = edge.lty, font = font, cex = cex, adj = adj, srt = srt,
# no.margin = no.margin, root.edge = root.edge, label.offset = label.offset,
# underscore = underscore, x.lim = x.lim, y.lim = y.lim, direction = direction,
# lab4ut = lab4ut, tip.color = tip.color, plot = plot, rotate.tree = rotate.tree,
# open.angle = open.angle, lend = 2, new = FALSE)
## Modify the line 34 by adding `use.edge.length = FALSE`
body(plotBranchbyTrait_no_edge_length)[[34]] <- substitute( xx <- plot.phylo(use.edge.length = FALSE, tree, type = type, show.tip.label = show.tip.label, show.node.label = show.node.label, edge.color = colors, edge.width = edge.width, edge.lty = edge.lty, font = font, cex = cex, adj = adj, srt = srt, no.margin = no.margin, root.edge = root.edge, label.offset = label.offset, underscore = underscore, x.lim = x.lim, y.lim = y.lim, direction = direction, lab4ut = lab4ut, tip.color = tip.color, plot = plot, rotate.tree = rotate.tree, open.angle = open.angle, lend = 2, new = FALSE) )
## Testing whether it worked
library(phytools)
tree <- pbtree(n=50)
x <- fastBM(tree)
## With use.edge.length = TRUE (default)
plotBranchbyTrait(tree, x, mode = "tips", edge.width = 4, prompt = FALSE)
## With use.edge.length = FALSE
plotBranchbyTrait_no_edge_length(tree, x, mode = "tips", edge.width = 4, prompt = FALSE)
You can find more on how to modify functions here.

How to change the color and width of lines with par function in R

I have a question about the par function in R.
I want to change the color and/or width of a line in a graph with par function. (I am using par function because the gaps.plot command below does not allow "col" option to be included. The gaps.plot command is used after the synth command).
So, I used the following command. But I noticed that the lines of the BOX are changed rather than the lines of the GRAPHS.
synth1<-read.csv(file="C:\\Users\\Research\\R\\synthinR_v4.csv",header=TRUE)
attach(synth1)
library("Synth")
dataprep.out34 <- dataprep(foo = synth1, predictors = c("lncdsales", "md1", "md2","md3", "md4", "md5", "md6", "md7", "md8", "md9", "md10", "md11", "yd1", "yd2", "yd3", "yd4", "yd5", "yd6", "yd7", "yd8"), predictors.op = "mean", time.predictors.prior = -13:1, dependent = "lndigital", unit.variable = "artistalbumcode", time.variable = "release", treatment.identifier = 34, controls.identifier = c(1:33, 35:49), time.optimize.ssr = -13:1, time.plot = -13:25)
synth.out34 <- synth(data.prep.obj = dataprep.out34, method = "BFGS")
par(lwd = 2, col="#cccccc")
gaps.plot(synth.res = synth.out34, dataprep.res = dataprep.out34, Ylab = " Log Digital Sales ", Xlab = "Release", Ylim = c(-7, 7) , Main = NA)
Does anyone know how to fix this problem??
Thank you in advance for your willingness to help. I greatly appreciate it!
The col argument to par sets the default plotting colour (i.e. when col is not explicitly specified in plotting calls), but unfortunately col = "black" is hard-coded into the source of gaps.plot.
You can make a modified copy of the function by either (1) viewing the source (F2 in RStudio, or just executing gaps.plot), editing it and assigning it to a new object, or (2) doing something like the following:
gaps.plot2 <- eval(parse(text=gsub('col = "black"', 'col = "red"',
deparse(Synth:::gaps.plot))))
and then using gaps.plot2 as you would use gaps.plot:
gaps.plot2(synth.res = synth.out34, dataprep.res = dataprep.out34,
Ylab = " Log Digital Sales ", Xlab = "Release", Ylim = c(-7, 7) ,
Main = NA)
Alter the lwd similarly. For example to make lines red and have width of 3, use nested gsub calls like this:
gaps.plot2 <- eval(parse(text=gsub('lwd = 2', 'lwd = 3',
gsub('col = "black"', 'col = "red"',
deparse(Synth:::gaps.plot)))))

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