I have conducted a study with triplicates (SampleID) for each sample (Sample) on different time points.
Now, I want to plot the means of the triplicates for the characteristic "Aerobic".
I want to plot for example the development of amount of aerobic bacteria over time. Therefore, I need to calculate the means (and the standard deviation) of the triplicates and then plot these means in the graph. Here, I could imagine to use a geom_line or geom_point diagram.
SampleID Sample Aerobic Anaerobic Day
[Factor] [Factor] [num] [num] [num]
1 V1.1.K1 V1.1.K 0.610063430 0.05146154 1
2 V1.1.K2 V1.1.K 0.740887757 0.02115290 1
3 V1.1.K3 V1.1.K 0.683726217 0.04270182 1
4 V1.1.N1 V1.1.N 0.432019752 0.35722350 1
5 V1.1.N2 V1.1.N 0.515792694 0.41357935 1
6 V1.14.K16 V1.14.K 0.038141335 0.84496088 14
7 V1.14.K17 V1.14.K 0.042078682 0.76523093 14
8 V1.14.K18 V1.14.K 0.009594763 0.90767637 14
9 V1.14.N0 V1.14.N 0.513100502 0.10618731 14
10 V1.14.W16 V1.14.W 0.483710571 0.32765968 14
How should i do this?
I tried it with the following code
plot <- mydata %>%
group_by(Sample) %>%
mutate(Mean=mean(Aerobic)) %>%
ggplot(aes(x=Day, y=Aerobic)) +
geom_point()
If I google the questions I get only information about how to calculate the mean alone, but not to set up a new table with the means for the different variables.
Is there something like
calc_mean_by_group ??
You would help me a lot :)
Simple base-R solution for calculating the means:
tapply(X = foo$Aerobic, INDEX = foo$Sample, FUN = mean)
("foo" being the name of your data.frame)
Related
I have a specific use problem. I want to sample exact sizes from within groups. What method should I use to construct exact subsets based on group counts?
My use case is that I am going through a two-stage sample design. First, for each group in my population, I want to ensure that 60% of subjects will not be selected. So I am trying to construct a sampling data frame that excludes 60% of available subjects for each group. Further, this is a function where the user specifies the minimum proportion of subjects that must not be used, hence the 1- construction where the user has indicated that at least 60% of subjects in each group cannot be selected for sampling.
After this code, I will be sampling completely at random, to get my final sample.
Code example:
testing <- data.frame(ID = c(seq_len(50)), Age = c(rep(18, 10), rep(19, 9), rep(20,15), rep(21,16)))
testing <- testing %>%
slice_sample(ID, prop=1-.6)
As you can see, the numbers by group are not what I want. I should only have 4 subjects who are 18 years of age, 3 subjects who are 19 years, 6 subjects who are 20 years of age, and 6 subjects who are 21 years of age. With no set seed, the numbers I ended up with were 6 18-year-olds, 1 19-year-old, 6 20-year-olds, and 7 21-year-olds.
However, the overall sample size of 20 is correct.
How do I brute force the sample size within the groups to be what I need?
There are other variables in the data frame so I need to sample randomly from each age group.
EDIT: Messed up trying to give an example. In my real data I am grouping by age inside the dplyr set of commands. But neither group-by([Age variable) ahead of slice_sample() or doing the grouping inside slice_sample() work. In my real data, I get neither the correct set of samples by age, nor do I get the correct overall sample size.
I was using a semi_join to limit the ages to those that had a total remaining after doing the proportion test. For those ages for which no sample could be taken, the semi_join was being used to remove those ages from the population ahead of doing the proportional sampling. I don't know if the semi_join has caused the problem.
That said, the answer provided and accepted shifts me away from relying on the semi_join and I think is an overall large improvement to my real code.
You haven't defined your grouping variable.
Try the following:
set.seed(1)
x <- testing %>% group_by(Age) %>% slice_sample(prop = .4)
x %>% count()
# # A tibble: 4 x 2
# # Groups: Age [4]
# Age n
# <dbl> <int>
# 1 18 4
# 2 19 3
# 3 20 6
# 4 21 6
Alternatively, try stratified from my "splitstackshape" package:
library(splitstackshape)
set.seed(1)
y <- stratified(testing, "Age", .4)
y[, .N, Age]
# Age N
# 1: 18 4
# 2: 19 4
# 3: 20 6
# 4: 21 6
How can I plot a recurrency in R.
Any solution with base plot, ggplot2, lattice, or a dedicated package is welcome.
For example:
Imagine I have these data:
mydata <- data.frame(t=1:10, Y=runif(10))
t Y
1 0.3744869
2 0.6314202
3 0.3900789
4 0.6896278
5 0.6894134
6 0.5549006
7 0.4296244
8 0.4527201
9 0.3064433
10 0.5783539
I could transform it like this:
mydata2 <- data.frame(t=c(NA,mydata$t),Y=c(NA,mydata$Y),Y2=c(mydata$Y, NA))
t Y Y2
NA NA 0.9103703
1 0.9103703 0.1426041
2 0.1426041 0.4150476
3 0.4150476 0.2109258
4 0.2109258 0.4287504
5 0.4287504 0.1326900
6 0.1326900 0.4600964
7 0.4600964 0.9429571
8 0.9429571 0.7619739
9 0.7619739 0.9329098
10 0.9329098 NA
(or similar methods, but I can have problems with missing data)
And plot it
plot(Y2~Y, data=mydata2)
I guess I must use some grouping function such as ave or apply. But it's not an elegant solution, and if I have more columns it can become difficult to generalize the transformation.
For example
mydata3 <- data.frame(x=sample(10,100, replace=T),t=1:100, Y=2*runif(100)+1)
For every x (or combination of values on other columns) I want to plot Y_{i+1} ~ Y_i, on the same plot.
Other tools, such as Mathematica have functions to plot sequences directly.
I've found a solution, thoug not very beautiful:
For this sample data.
mydata <- data.frame(x=sample(4,25, replace=T),t=1:25, Y=2*runif(25)+1)
newdata <- mydata[order(mydata$x, mydata$t), ]
newdata$prev <- ave(newdata$Y, newdata$x, FUN=function(x) c(NA,head(x,-1)))
plot(Y~prev, data=newdata)
In this example you don't have rows for every t value, you would need to first generate NAs for missing values. But it's just a quick solution. In my real data I have many observations for each t.
lag.plot can plot recurrence plots but not within each subgroup.
I am a novice R user, hence the question. I refer to the solution on creating stacked barplots from R programming: creating a stacked bar graph, with variable colors for each stacked bar.
My issue is slightly different. I have 4 column data. The last column is the summed total of the first 3 column. I want to plot bar charts with the following information 1) the summed total value (ie 4th column), 2) each bar is split by the relative contributions of each of the three column.
I was hoping someone could help.
Regards,
Bernard
If I understood it rightly, this may do the trick
the following code works well for the example df dataframe
df <- a b c sum
1 9 8 18
3 6 2 11
1 5 4 10
23 4 5 32
5 12 3 20
2 24 1 27
1 2 4 7
As you don't want to plot a counter of variables, but the actual value in your dataframe, you need to use the goem_bar(stat="identity") method on ggplot2. Some data manipulation is necessary too. And you don't need a sum column, ggplot does the sum for you.
df <- df[,-ncol(df)] #drop the last column (assumed to be the sum one)
df$event <- seq.int(nrow(df)) #create a column to indicate which values happaned on the same column for each variable
df <- melt(df, id='event') #reshape dataframe to make it readable to gpglot
px = ggplot(df, aes(x = event, y = value, fill = variable)) + geom_bar(stat = "identity")
print (px)
this code generates the plot bellow
Consider the following frequency data:
> table(income)
income
3 5 6 7 8 5000
2 7 2 2 2 1
When I type >hist(income) I get the following histogram
So as you can see, the fact that most income values are concentrated around 5 and there is one value quite distant from the others makes the histogram not look very good. MS Excel can consider the 5000 value as of another category, so the data would like this instead:
> table(income)
income
3 5 6 7 8 more
2 7 2 2 2 1
So plotting this as a histogram would look much better, so you can see the frequency within a shorter range:
Is there anyway to do this either with the hist() function or others functions from lattice or ggplot2? I do however, don't want to overwrite the values that exceed a certain threshold, so as I do lose any information.
Thanks a lot!
Data generation:
income <- c(rep(3,2), rep(5,7), rep(6,2), rep(7,2), rep(8,2), 5000)
Function for preparing data for plotting:
nice.data <- function(x, threshold=10){
x[x>threshold] <- "More"
x
}
Plotting:
library(ggplot2)
ggplot() + geom_histogram(aes(x=nice.data(income))) + xlab("Income")
Result:
I have some data that I want to display as a box plot using ggplot2. It's basically counts, stratified by two other variables. Here's an example of the data (in reality there's a lot more, but the structure is the same):
TAG Count Condition
A 5 1
A 6 1
A 6 1
A 6 2
A 7 2
A 7 2
B 1 1
B 2 1
B 2 1
B 12 2
B 8 2
B 10 2
C 10 1
C 12 1
C 13 1
C 7 2
C 6 2
C 10 2
For each Tag, there are a fixed number of observations in condition 1, and condition 2 (here it's 3, but in the real data it's much more). I want a box plot like the following ('s' is a dataframe arranged as above):
ggplot(s, aes(x=TAG, y=Count, fill=factor(Condition))) + geom_boxplot()
This is fine, but I want to be able to order the x-axis by the p-value from a Wilcoxon test for each Tag. For example, with the above data, the values would be (for the tags A,B, and C respectively):
> wilcox.test(c(5,6,6),c(6,7,7))$p.value
[1] 0.1572992
> wilcox.test(c(1,2,2),c(12,8,10))$p.value
[1] 0.0765225
> wilcox.test(c(10,12,13),c(7,6,10))$p.value
[1] 0.1211833
Which would induce the ordering A,C,B on the x-axis (largest to smallest). But I don't know how to go about adding this information into my data (specifically, attaching a p-value at just the tag level, rather than adding a whole extra column), or how to use it to change the x-axis order. Any help greatly appreciated.
Here is a way do it. The first step is to calculate the p-values for each TAG. We do this by using ddply which splits the data by TAG, and calculates the p-value using the formula interface to wilcox.test. The plot statement reorders the TAG based on its p-value.
library(ggplot2); library(plyr)
dfr2 <- ddply(dfr, .(TAG), transform,
pval = wilcox.test(Count ~ Condition)$p.value)
qplot(reorder(TAG, pval), Count, fill = factor(Condition), geom = 'boxplot',
data = dfr2)