T-SNE code text labelling of the clusters - r

Im using this code for running t-sne .
I want to do the t-sne on my whole data frame
So is there way to label my points that are being clustered and as well as label them with different colours to make them visually differentiable .
These are my samples CMP_6792" "CMP_7256" "CMP_7653" "GMP_6792" "GMP_7256" "GMP_7653" "HSC_6792"
"HSC_7256" "HSC_7653" "Mono_6792" "Mono_7256" "Mono_7653" "Gran1" "Gran2
I would like to label my points according to the above mentioned sample.
Here is my code
file1<- read.csv('PRIMARY_CELL_EPILIST.csv')
head(file1)
names(file1)
class(file1)
dat <- data.frame(file1)
rownames(file1) <- make.names(file1[,1], unique = TRUE)
head(file1)
dim(file1)
data <- file1[,2:15]
head(data)
library(tsne)
tsne1 <- tsne(scale(data), perplexity = 10,max_iter = 300)
plot(tsne1[, 1], tsne1[, 2])
library(ggplot2)
plotdata <- data.frame(tsne_x = tsne1[, 1], tsne_y = tsne1[, 2])
plt1 <- ggplot(plotdata) + geom_point(aes(x = tsne_x, y = tsne_y))
plot(plt1)
So any help or suggestion as well as improvement over my code would be highly appreciated .

You will first want to cluster your t-SNE results. The cluster assignments will then serve as color assignment.
cl <- cluster::pam( tsne1 )
Modify your plotdata data.frame so that it includes everything (sample names, t-SNE coordinates, cluster assignments):
plotdata <- data.frame( tsne_x = tsne1[,1], tsne_y = tsne1[,2], SampleID = v,
Cluster = cl$clustering )
where v is the vector of sample names you provided (i.e., v <- c( "CMP_6792", "CMP_7256", "CMP_7653", ... ), or v <- rownames(tsne1) if it's available).
Finally, adjust your ggplot call to access the relevant columns in the data.frame:
plt1 <- ggplot( plotdata, aes( x = tsne_x, y = tsne_y, color = Cluster ) +
geom_point() + ggrepel::geom_text_repel( aes( label = SampleID ) )

Related

How to specify groups with colors in qqplot()?

I have created a qqplot (with quantiles of beta distribution) from a dataset including two groups. To visualize, which points belong to which group, I would like to color them. I have tried the following:
res <- beta.mle(data$values) #estimate parameters of beta distribution
qqplot(qbeta(ppoints(500),res$param[1], res$param[2]),data$values,
col = data$group,
ylab = "Quantiles of data",
xlab = "Quantiles of Beta Distribution")
the result is shown here:
I have seen solutions specifying a "col" vector for qqnorm, hover this seems to not work with qqplot, as simply half the points is colored in either color, regardless of group. Is there a way to fix this?
A simulated some data just to shown how to add color in ggplot
Libraries
library(tidyverse)
# install.packages("Rfast")
Data
#Simulating data from beta distribution
x <- rbeta(n = 1000,shape1 = .5,shape2 = .5)
#Estimating parameters
res <- Rfast::beta.mle(x)
data <-
tibble(
simulated_data = sort(x),
quantile_data = qbeta(ppoints(length(x)),res$param[1], res$param[2])
) %>%
#Creating a group variable using quartiles
mutate(group = cut(x = simulated_data,
quantile(simulated_data,seq(0,1,.25)),
include.lowest = T))
Code
data %>%
# Adding group variable as color
ggplot(aes( x = quantile_data, y = simulated_data, col = group))+
geom_point()
Output
For those who are wondering, how to work with pre-defined groups, this is the code that worked for me:
library(tidyverse)
library(Rfast)
res <- beta.mle(x)
# make sure groups are not numerrical
# (else color skale might turn out continuous)
g <- plyr::mapvalues(g, c("1", "2"), c("Group1", "Group2"))
data <-
tibble(
my_data = sort(x),
quantile_data = qbeta(ppoints(length(x)),res$param[1], res$param[2]),
group = g[order(x)]
)
data %>%
# Adding group variable as color
ggplot(aes( x = quantile_data, y = my_data, col = group))+
geom_point()
result

Changing aesthetics in ggplot generated by svars package in R

I'm using the svars package to generate some IRF plots. The plots are rendered using ggplot2, however I need some help with changing some of the aesthetics.
Is there any way I can change the fill and alpha of the shaded confidence bands, as well as the color of the solid line? I know in ggplot2 you can pass fill and alpha arguments to geom_ribbon (and col to geom_line), just unsure of how to do the same within the plot function of this package's source code.
# Load Dataset and packages
library(tidyverse)
library(svars)
data(USA)
# Create SVAR Model
var.model <- vars::VAR(USA, lag.max = 10, ic = "AIC" )
svar.model <- id.chol(var.model)
# Wild Bootstrap
cores <- parallel::detectCores() - 1
boot.svar <- wild.boot(svar.model, n.ahead = 30, nboot = 500, nc = cores)
# Plot the IRFs
plot(boot.svar)
I'm also looking at the command for a historical decomposition plot (see below). Is there any way I could omit the first two facets and plot only the bottom three lines on the same facet?
hist.decomp <- hd(svar.model, series = 1)
plot(hist.decomp)
Your first desired result is easily achieved by resetting the aes_params after calling plot. For your second goal. There is probably an approach to manipulate the ggplot object. Instead my approach below constructs the plot from scratch. Basically I copy and pasted the data wrangling code from vars:::plot.hd and filtered the prepared dataset for the desired series:
# Plot the IRFs
p <- plot(boot.svar)
p$layers[[1]]$aes_params$fill <- "pink"
p$layers[[1]]$aes_params$alpha <- .5
p$layers[[2]]$aes_params$colour <- "green"
p
# Helper to convert to long dataframe. Source: svars:::plot.hd
hd2PlotData <- function(x) {
PlotData <- as.data.frame(x$hidec)
if (inherits(x$hidec, "ts")) {
tsStructure = attr(x$hidec, which = "tsp")
PlotData$Index <- seq(from = tsStructure[1], to = tsStructure[2],
by = 1/tsStructure[3])
PlotData$Index <- as.Date(yearmon(PlotData$Index))
}
else {
PlotData$Index <- 1:nrow(PlotData)
PlotData$V1 <- NULL
}
dat <- reshape2::melt(PlotData, id = "Index")
dat
}
hist.decomp <- hd(svar.model, series = 1)
dat <- hd2PlotData(hist.decomp)
dat %>%
filter(grepl("^Cum", variable)) %>%
ggplot(aes(x = Index, y = value, color = variable)) +
geom_line() +
xlab("Time") +
theme_bw()
EDIT One approach to change the facet labels is via a custom labeller function. For a different approach which changes the facet labels via the data see here:
myvec <- LETTERS[1:9]
mylabel <- function(labels, multi_line = TRUE) {
data.frame(variable = labels)
}
p + facet_wrap(~variable, labeller = my_labeller(my_labels))

Get multiple polygons for scattered data in R

I have point cloud data of an area (x,y,z coordinates)
The plot of X and Y looks like:
I am trying to get polygons of different clusters in this data. I tried the following:
points <- df [,1:2] # x and y coordinates
pts <- st_as_sf(points, coords=c('X','Y'))
conc <- concaveman(pts, concavity = 0.5, length_threshold = 0)
Seems like I just get a single polygon binding the whole data. conc$polygons is a list of one variable.
How can I define multiple polygons? What am I missing when I am using concaveman and what all it can provide?
It's hard to tell from your example what variable defines your clusters. Below is an example with some simulated clusters using ggplot2 and data.table (adapted from here).
library(data.table)
library(ggplot2)
# Simulate data:
set.seed(1)
n_cluster = 50
centroids = cbind.data.frame(
x=rnorm(5, mean = 0, sd=5),
y=rnorm(5, mean = 0, sd=5)
)
dt = rbindlist(
lapply(
1:nrow(centroids),
function(i) {
cluster_dt = data.table(
x = rnorm(n_cluster, mean = centroids$x[i]),
y = rnorm(n_cluster, mean = centroids$y[i]),
cluster = i
)
}
)
)
dt[,cluster:=as.factor(cluster)]
# Find convex hull of each point by cluster:
hulls = dt[,.SD[chull(x,y)],by=.(cluster)]
# Plot:
p = ggplot(data = dt, aes(x=x, y=y, colour=cluster)) +
geom_point() +
geom_polygon(data = hulls,aes(fill=cluster,alpha = 0.5)) +
guides(alpha=F)
This produces the following output:
Edit
If you don't have predefined clusters, you can use a clustering algorithm. As a simple example, see below for a solution using kmeans with 5 centroids.
# Estimate clusters (e.g. kmeans):
dt[,km_cluster := as.factor(kmeans(.SD,5)$cluster),.SDcols=c("x","y")]
# Find convex hull of each point:
hulls = dt[,.SD[chull(x,y)],by=.(km_cluster)]
# Plot:
p = ggplot(data = dt, aes(x=x, y=y, colour=km_cluster)) +
geom_point() +
geom_polygon(data = hulls,aes(fill=km_cluster,alpha = 0.5)) +
guides(alpha=F)
In this case the output for the estimated clusters is almost equivalent to the constructed ones.

How to graph “before and after” measures using ggplot with connecting lines?

I’m new to R I want to create a ”before-and-after” scatterplot with connecting lines to illustrate the different power outputs before and after a training intervention.
I want something like the graph in the picture.
Example
Sample Data
X <- c(0,1,0,1,0,1,0,1) # 0=before, 1=after
y <- c(1001,1030,900,950,1040,1020,1010,1000) #Power output
Group <- c(0,0,0,0,1,1,1,1) # 0=Control 1=Experimental
id <- c(1,1,2,2,3,3,4,4) # id = per individual
df <- data.frame(x,y,Group,id)
Many thanks
x <- c(0,1,0,1,0,1,0,1) # 0=before, 1=after
y <- c(1001,1030,900,950,1040,1020,1010,1000) #Power output
Group <- c(0,0,0,0,1,1,1,1) # 0=Control 1=Experimental
id <- c(1,1,2,2,3,3,4,4) # id = per individual
df <- dplyr::bind_cols(
x = x,
y = y,
Group = as.factor(Group),
id = as.factor(id)
)
library(ggplot2)
ggplot(df) +
aes(x,y, color = Group, shape = Group, group = id)+
geom_point()+
geom_line()

created a nested cdf that doesn't reach 1

Here is some workable example of data I wish to plot:
set.seed(123)
x <- rweibull(n = 2000, shape = 2, scale = 10)
x <- round(x, digits = 0)
x <- sort(x, decreasing = FALSE)
y <- c(rep(0.1, times = 500),rep(0.25, times = 500),rep(0.4, times = 500),rep(0.85, times = 500))
z <- rbinom(n=2000, size=1, prob=y)
df1 <- data.frame(x,z)
I want to plot the overal fequency of z across x.
unlike a typical cdf, the function should not reach 1.0, but instead
sum(df1$z)/length(df1$z)
a ymax of 0.36 (721/2000).
using ggplot2 we can create a cdf of x with the following command:
library(ggplot2)
ggplot(df1, aes(x)) + stat_ecdf()
But i want to extend this plot to show the cumulative percentage of z (as a function of 'x')
The end result should like like
EDIT
with some very poor data manipulation I am able to generate the something similiar to a cdf plot, but there must be a more beautiful and easy method using various packages and ggplot
mytable <- table(df1$x, df1$z)
mydf <- as.data.frame.matrix(mytable)
colnames(mydf) <- c("z_no", "z_yes")
mydf$A <- 1:length(mydf$z_no)
mydf$sum <- cumsum(mydf$z_yes)
mydf$dis <- mydf$sum/length(z)
plot(mydf$A, mydf$dis)
You can use the package dplyr to process the data as follows:
library(dplyr)
plot_data <- group_by(df1, x) %>%
summarise(z_num = sum(z)) %>%
mutate(cum_perc_z = cumsum(z_num)/nrow(df1))
This gives the same result as the data processing that you describe in your edit. Note, however, that I get sum(df1$z) = 796 and the maximal y value is thus 796/2000 = 0.398.
For the plot, you can use geom_step() to have a step function and add the horizontal line with geom_hline():
ggplot(plot_data, aes(x = x, y = cum_perc_z)) +
geom_step(colour = "red", size = 0.8) +
geom_hline(yintercept = max(plot_data$cum_perc_z))

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