Related
I used this code to calculate relative abundance (cell/total of column) of a table I had. I don't understand how the . and ~ functions work.
The construct ~./sum(x) is technically a special type of R object called a formula
class(~./sum(x))
#> [1] "formula"
However, in tidyverse functions such as mutate_all, this formula is taken and converted into a lambda function, which is an anonymous function (i.e. a function that isn't named and is written in place as a parameter passed in a call to another function).
Internally, the formula is converted into a function with rlang::as_function. Suppose we wanted to write a function that just adds two to a variable. In base R we might write
add_two <- function(var){
return(var + 2)
}
add_two(5)
#> [1] 7
In the tidyverse, we can use a formula as shorthand for this function, where the . becomes a shorthand for "the variable that was passed as a first argument to the function":
add_two <- rlang::as_function(~ . + 2)
add_two(5)
#> [1] 7
In functions such as mutate_all, the formula will automatically be passed through rlang::as_function, so if we wanted to add two to each column in our data frame, instead of writing:
mutate_all(.funs= function(var) {return(var + 2);})
we could write
mutate_all(.funs=~.+2)
In your case, the formula ~./sum(x) is effectively transformed into
function(var) {
return(var / sum(x))
}
where x has to exist either as a column in your data frame or a variable in the calling environment.
The reasons for having it this way are that it saves typing and shortens lines of code. Inserting a function within a call to another function often leads to messy and poorly formatted code. This shorthand method helps to prevent that.
You can read more about anonymous functions and how they are used in the tidyverse here
Suppose we have this dataset:
dataset <- data.frame(a = c(1,2,3,4),
b = c(2,3,4,5),
c = c(3,4,5,6))
And you want to divide all vectors by the total (ie. for vector a = 1/10, 2/10, 3/10, 4/10). To avoid writing for all variables, you can use mutate_all, and then a lambda using .funswhich says make a function that divides all values in each vector represented by the dot by the sum of all values in that vector.
dataset %>% mutate_all(.funs = ~./sum(.))
Hope this helps.
mutate_all applies the function in .funs to all values. Each value (.) is divided by the sum(x), to get you the "relative abundance" which is essentially the fraction of the total value, which is the sum(x). You can think of ~ as a "function of". So you are saying each cell in the dataframe is a function of itself divided by the overall sum.
In the official docs, it says:
substitute returns the parse tree for the (unevaluated) expression
expr, substituting any variables bound in env.
quote simply returns its argument. The argument is not evaluated and
can be any R expression.
But when I try:
> x <- 1
> substitute(x)
x
> quote(x)
x
It looks like both quote and substitute returns the expression that's passed as argument to them.
So my question is, what's the difference between substitute and quote, and what does it mean to "substituting any variables bound in env"?
Here's an example that may help you to easily see the difference between quote() and substitute(), in one of the settings (processing function arguments) where substitute() is most commonly used:
f <- function(argX) {
list(quote(argX),
substitute(argX),
argX)
}
suppliedArgX <- 100
f(argX = suppliedArgX)
# [[1]]
# argX
#
# [[2]]
# suppliedArgX
#
# [[3]]
# [1] 100
R has lazy evaluation, so the identity of a variable name token is a little less clear than in other languages. This is used in libraries like dplyr where you can write, for instance:
summarise(mtcars, total_cyl = sum(cyl))
We can ask what each of these tokens means: summarise and sum are defined functions, mtcars is a defined data frame, total_cyl is a keyword argument for the function summarise. But what is cyl?
> cyl
Error: object 'cyl' not found
It isn't anything! Well, not yet. R doesn't evaluate it right away, but treats it as an expression to be parsed later with some parse tree that is different than the global environment your command line is working in, specifically one where the columns of mtcars are defined. Somewhere in the guts of dplyr, something like this is happening:
> substitute(cyl, mtcars)
[1] 6 6 4 6 8 ...
Suddenly cyl means something. That's what substitute is for.
So what is quote for? Well sometimes you want your lazily-evaluated expression to be represented somewhere else before it's evaluated, i.e. you want to display the actual code you're writing without any (or only some) values substituted. The docs you quoted explain this is common for "informative labels for data sets and plots".
So, for example, you could create a quoted expression, and then both print the unevaluated expression in your chart to show how you calculated and actually calculate with the expression.
expr <- quote(x + y)
print(expr) # x + y
eval(expr, list(x = 1, y = 2)) # 3
Note that substitute can do this expression trick also while giving you the option to parse only part of it. So its features are a superset of quote.
expr <- substitute(x + y, list(x = 1))
print(expr) # 1 + y
eval(expr, list(y = 2)) # 3
Maybe this section of the documentation will help somewhat:
Substitution takes place by examining each component of the parse tree
as follows: If it is not a bound symbol in env, it is unchanged. If it
is a promise object, i.e., a formal argument to a function or
explicitly created using delayedAssign(), the expression slot of the
promise replaces the symbol. If it is an ordinary variable, its value
is substituted, unless env is .GlobalEnv in which case the symbol is
left unchanged.
Note the final bit, and consider this example:
e <- new.env()
assign(x = "a",value = 1,envir = e)
> substitute(a,env = e)
[1] 1
Compare that with:
> quote(a)
a
So there are two basic situations when the substitution will occur: when we're using it on an argument of a function, and when env is some environment other than .GlobalEnv. So that's why you particular example was confusing.
For another comparison with quote, consider modifying the myplot function in the examples section to be:
myplot <- function(x, y)
plot(x, y, xlab = deparse(quote(x)),
ylab = deparse(quote(y)))
and you'll see that quote really doesn't do any substitution.
Regarding your question why GlobalEnv is treated as an exception for substitute, it is just a heritage of S. From The R language definition (https://cran.r-project.org/doc/manuals/r-release/R-lang.html#Substitutions):
The special exception for substituting at the top level is admittedly peculiar. It has been inherited from S and the rationale is most likely that there is no control over which variables might be bound at that level so that it would be better to just make substitute act as quote.
I am a beginner so I'd appreciate any thoughts, and I understand that this question might be too basic for some of you.
Also, this question is not about the difference between <- and =, but about the way they get evaluated when they are part of the function argument. I read this thread, Assignment operators in R: '=' and '<-' and several others, but I couldn't understand the difference.
Here's the first line of code:
My objective is to get rid of variables in the environment. From reading the above thread, I would believe that <- would exist in the user workspace, so there shouldn't be any issue with deleting all variables.
Here is my code and two questions:
Question 1
First off, this code doesn't work.
rm(ls()) #throws an error
I believe this happens because ls() returns a character vector, and rm() expects an object name. Am I correct? If so, I would appreciate if someone could guide me how to get object names from character array.
Question 2
I googled this topic and found that this code below deletes all variables.
rm(list = ls())
While this does help me, I am unsure why = is used instead of <-. If I run the following code, I get an error Error in rm(list <- ls()) : ... must contain names or character strings
rm(list <- ls())
Why is this? Can someone please guide me? I'd appreciate any help/guidance.
I read this thread, Assignment operators in R: '=' and '<-' and several others, but I couldn't understand the difference.
No wonder, since the answers there are actually quite confusing, and some are outright wrong. Since that’s the case, let’s first establish the difference between them before diving into your actual question (which, it turns out, is mostly unrelated):
<- is an assignment operator
In R, <- is an operator that performs assignment from right to left, in the current scope. That’s it.
= is either an assignment operator or a distinct syntactic token
=, by contrast, has several meanings: its semantics change depending on the syntactic context it is used in:
If = is used inside a parameter list, immediately to the right of a parameter name, then its meaning is: “associate the value on the right with the parameter name on the left”.
Otherwise (i.e. in all other situations), = is also an operator, and by default has the same meaning as <-: i.e. it performs assignment in the current scope.
As a consequence of this, the operators <- and = can be used interchangeably1. However, = has an additional syntactic role in an argument list of a function definition or a function call. In this context it’s not an operator and cannot be replaced by <-.
So all these statements are equivalent:
x <- 1
x = 1
x[5] <- 1
x[5] = 1
(x <- 1)
(x = 1)
f((x <- 5))
f((x = 5))
Note the extra parentheses in the last example: if we omitted these, then f(x = 5) would be interpreted as a parameter association rather than an assignment.
With that out of the way, let’s turn to your first question:
When calling rm(ls()), you are passing ls() to rm as the ... parameter. Ronak’s answer explains this in more detail.
Your second question should be answered by my explanation above: <- and = behave differently in this context because the syntactic usage dictates that rm(list = ls()) associates ls() with the named parameter list, whereas <- is (as always) an assignment operator. The result of that assignment is then once again passed as the ... parameter.
1 Unless somebody changed their meaning: operators, like all other functions in R, can be overwritten with new definitions.
To expand on my comment slightly, consider this example:
> foo <- function(a,b) b+1
> foo(1,b <- 2) # Works
[1] 3
> ls()
[1] "b" "foo"
> foo(b <- 3) # Doesn't work
Error in foo(b <- 3) : argument "b" is missing, with no default
The ... argument has some special stuff going on that restricts things a little further in the OP's case, but this illustrates the issue with how R is parsing the function arguments.
Specifically, when R looks for named arguments, it looks specifically for arg = val, with an equals sign. Otherwise, it is parsing the arguments positionally. So when you omit the first argument, a, and just do b <- 1, it thinks the expression b <- 1 is what you are passing for the argument a.
If you check ?rm
rm(..., list = character(),pos = -1,envir = as.environment(pos), inherits = FALSE)
where ,
... - the objects to be removed, as names (unquoted) or character strings (quoted).
and
list - a character vector naming objects to be removed.
So, if you do
a <- 5
and then
rm(a)
it will remove the a from the global environment.
Further , if there are multiple objects you want to remove,
a <- 5
b <- 10
rm(a, b)
This can also be written as
rm(... = a, b)
where we are specifying that the ... part in syntax takes the arguments a and b
Similarly, when we want to specify the list part of the syntax, it has to be given by
rm(list = ls())
doing list <- ls() will store all the variables from ls() in the variable named list
list <- ls()
list
#[1] "a" "b" "list"
I hope this is helpful.
I have two lists of lists. humanSplit and ratSplit. humanSplit has element of the form::
> humanSplit[1]
$Fetal_Brain_408_AGTCAA_L001_R1_report.txt
humanGene humanReplicate alignment RNAtype
66 DGKI Fetal_Brain_408_AGTCAA_L001_R1_report.txt 6 reg
68 ARFGEF2 Fetal_Brain_408_AGTCAA_L001_R1_report.txt 5 reg
If you type humanSplit[[1]], it gives the data without name $Fetal_Brain_408_AGTCAA_L001_R1_report.txt
RatSplit is also essentially similar to humanSplit with difference in column order. I want to apply fisher's test to every possible pairing of replicates from humanSplit and ratSplit. Now I defined the following empty vector which I will use to store the informations of my fisher's test
humanReplicate <- vector(mode = 'character', length = 0)
ratReplicate <- vector(mode = 'character', length = 0)
pvalue <- vector(mode = 'numeric', length = 0)
For fisher's test between two replicates of humanSplit and ratSplit, I define the following function. In the function I use `geneList' which is a data.frame made by reading a file and has form:
> head(geneList)
human rat
1 5S_rRNA 5S_rRNA
2 5S_rRNA 5S_rRNA
Now here is the main function, where I use a function getGenetype which I already defined in other part of the code. Also x and y are integers :
fishertest <-function(x,y) {
ratReplicateName <- names(ratSplit[x])
humanReplicateName <- names(humanSplit[y])
## merging above two based on the one-to-one gene mapping as in geneList
## defined above.
mergedHumanData <-merge(geneList,humanSplit[[y]], by.x = "human", by.y = "humanGene")
mergedRatData <- merge(geneList, ratSplit[[x]], by.x = "rat", by.y = "ratGene")
## [here i do other manipulation with using already defined function
## getGenetype that is defined outside of this function and make things
## necessary to define following contingency table]
contingencyTable <- matrix(c(HnRn,HnRy,HyRn,HyRy), nrow = 2)
fisherTest <- fisher.test(contingencyTable)
humanReplicate <- c(humanReplicate,humanReplicateName )
ratReplicate <- c(ratReplicate,ratReplicateName )
pvalue <- c(pvalue , fisherTest$p)
}
After doing all this I do the make matrix eg to use in apply. Here I am basically trying to do something similar to double for loop and then using fisher
eg <- expand.grid(i = 1:length(ratSplit),j = 1:length(humanSplit))
junk = apply(eg, 1, fishertest(eg$i,eg$j))
Now the problem is, when I try to run, it gives the following error when it tries to use function fishertest in apply
Error in humanSplit[[y]] : recursive indexing failed at level 3
Rstudio points out problem in following line:
mergedHumanData <-merge(geneList,humanSplit[[y]], by.x = "human", by.y = "humanGene")
Ultimately, I want to do the following:
result <- data.frame(humanReplicate,ratReplicate, pvalue ,alternative, Conf.int1, Conf.int2, oddratio)
I am struggling with these questions:
In defining fishertest function, how should I pass ratSplit and humanSplit and already defined function getGenetype?
And how I should use apply here?
Any help would be much appreciated.
Up front: read ?apply. Additionally, the first three hits on google when searching for "R apply tutorial" are helpful snippets: one, two, and three.
Errors in fishertest()
The error message itself has nothing to do with apply. The reason it got as far as it did is because the arguments you provided actually resolved. Try to do eg$i by itself, and you'll see that it is returning a vector: the corresponding column in the eg data.frame. You are passing this vector as an index in the i argument. The primary reason your function erred out is because double-bracket indexing ([[) only works with singles, not vectors of length greater than 1. This is a great example of where production/deployed functions would need type-checking to ensure that each argument is a numeric of length 1; often not required for quick code but would have caught this mistake. Had it not been for the [[ limit, your function may have returned incorrect results. (I've been bitten by that many times!)
BTW: your code is also incorrect in its scoped access to pvalue, et al. If you make your function return just the numbers you need and the aggregate it outside of the function, your life will simplify. (pvalue <- c(pvalue, ...) will find pvalue assigned outside the function but will not update it as you want. You are defeating one purpose of writing this into a function. When thinking about writing this function, try to answer only this question: "how do I compare a single rat record with a single human record?" Only after that works correctly and simply without having to overwrite variables in the parent environment should you try to answer the question "how do I apply this function to all pairs and aggregate it?" Try very hard to have your function not change anything outside of its own environment.
Errors in apply()
Had your function worked properly despite these errors, you would have received the following error from apply:
apply(eg, 1, fishertest(eg$i, eg$j))
## Error in match.fun(FUN) :
## 'fishertest(eg$i, eg$j)' is not a function, character or symbol
When you call apply in this sense, it it parsing the third argument and, in this example, evaluates it. Since it is simply a call to fishertest(eg$i, eg$j) which is intended to return a data.frame row (inferred from your previous question), it resolves to such, and apply then sees something akin to:
apply(eg, 1, data.frame(...))
Now that you see that apply is being handed a data.frame and not a function.
The third argument (FUN) needs to be a function itself that takes as its first argument a vector containing the elements of the row (1) or column (2) of the matrix/data.frame. As an example, consider the following contrived example:
eg <- data.frame(aa = 1:5, bb = 11:15)
apply(eg, 1, mean)
## [1] 6 7 8 9 10
# similar to your use, will not work; this error comes from mean not getting
# any arguments, your error above is because
apply(eg, 1, mean())
## Error in mean.default() : argument "x" is missing, with no default
Realize that mean is a function itself, not the return value from a function (there is more to it, but this definition works). Because we're iterating over the rows of eg (because of the 1), the first iteration takes the first row and calls mean(c(1, 11)), which returns 6. The equivalent of your code here is mean()(c(1, 11)) will fail for a couple of reasons: (1) because mean requires an argument and is not getting, and (2) regardless, it does not return a function itself (in a "functional programming" paradigm, easy in R but uncommon for most programmers).
In the example here, mean will accept a single argument which is typically a vector of numerics. In your case, your function fishertest requires two arguments (templated by my previous answer to your question), which does not work. You have two options here:
Change your fishertest function to accept a single vector as an argument and parse the index numbers from it. Bothing of the following options do this:
fishertest <- function(v) {
x <- v[1]
y <- v[2]
ratReplicateName <- names(ratSplit[x])
## ...
}
or
fishertest <- function(x, y) {
if (missing(y)) {
y <- x[2]
x <- x[1]
}
ratReplicateName <- names(ratSplit[x])
## ...
}
The second version allows you to continue using the manual form of fishertest(1, 57) while also allowing you to do apply(eg, 1, fishertest) verbatim. Very readable, IMHO. (Better error checking and reporting can be used here, I'm just providing a MWE.)
Write an anonymous function to take the vector and split it up appropriately. This anonymous function could look something like function(ii) fishertest(ii[1], ii[2]). This is typically how it is done for functions that either do not transform as easily as in #1 above, or for functions you cannot or do not want to modify. You can either assign this intermediary function to a variable (which makes it no longer anonymous, figure that) and pass that intermediary to apply, or just pass it directly to apply, ala:
.func <- function(ii) fishertest(ii[1], ii[2])
apply(eg, 1, .func)
## equivalently
apply(eg, 1, function(ii) fishertest(ii[1], ii[2]))
There are two reasons why many people opt to name the function: (1) if the function is used multiple times, better to define once and reuse; (2) it makes the apply line easier to read than if it contained a complex multi-line function definition.
As a side note, there are some gotchas with using apply and family that, if you don't understand, will be confusing. Not the least of which is that when your function returns vectors, the matrix returned from apply will need to be transposed (with t()), after which you'll still need to rbind or otherwise aggregrate.
This is one area where using ddply may provide a more readable solution. There are several tutorials showing it off. For a quick intro, read this; for a more in depth discussion on the bigger picture in which ddply plays a part, read Hadley's Split, Apply, Combine Strategy for Data Analysis paper from JSS.
I have:
z = data.frame(x1=a, x2=b, x3=c, etc)
I am trying to do:
for (i in 1:10)
{
paste(c('N'),i,sep="") -> paste(c('z$x'),i,sep="")
}
Problems:
paste(c('z$x'),i,sep="") yields "z$x1", "z$x1" instead of calling the actual values. I need the expression to be evaluated. I tried as.numeric, eval. Neither seemed to work.
paste(c('N'),i,sep="") yields "N1", "N2". I need the expression to be merely used as name. If I try to assign it a value such as paste(c('N'),5,sep="") -> 5, ie "N5" -> 5 instead of N5 -> 5, I get target of assignment expands to non-language object.
This task is pretty trivial since I can simply do:
N1 = x1...
N2 = x2...
etc, but I want to learn something new
I'd suggest using something like for( i in 1:10 ) z[,i] <- N[,i]...
BUT, since you said you want to learn something new, you can play around with parse and substitute.
NOTE: these little tools are funny, but experienced users (not me) avoid them.
This is called "computing on the language". It's very interesting, and it helps understanding the way R works. Let me try to give an intro:
The basic language construct is a constant, like a numeric or character vector. It is trivial because it is not different from its "unevaluated" version, but it is one of the building blocks for more complicated expressions.
The (officially) basic language object is the symbol, also known as a name. It's nothing but a pointer to another object, i.e., a token that identifies another object which may or may not exist. For instance, if you run x <- 10, then x is a symbol that refers to the value 10. In other words, evaluating the symbol x yields the numeric vector 10. Evaluating a non-existant symbol yields an error.
A symbol looks like a character string, but it is not. You can turn a string into a symbol with as.symbol("x").
The next language object is the call. This is a recursive object, implemented as a list whose elements are either constants, symbols, or another calls. The first element must not be a constant, because it must evaluate to the real function that will be called. The other elements are the arguments to this function.
If the first argument does not evaluate to an existing function, R will throw either Error: attempt to apply non-function or Error: could not find function "x" (if the first argument is a symbol that is undefined or points to something other than a function).
Example: the code line f(x, y+z, 2) will be parsed as a list of 4 elements, the first being f (as a symbol), the second being x (another symbol), the third another call, and the fourth a numeric constant. The third element y+z, is just a function with two arguments, so it parses as a list of three names: '+', y and z.
Finally, there is also the expression object, that is a list of calls/symbols/constants, that are meant to be evaluated one by one.
You'll find lots of information here:
https://github.com/hadley/devtools/wiki/Computing-on-the-language
OK, now let's get back to your question :-)
What you have tried does not work because the output of paste is a character string, and the assignment function expects as its first argument something that evaluates to a symbol, to be either created or modified. Alternativelly, the first argument can also evaluate to a call associated with a replacement function. These are a little trickier, but they are handled by the assignment function itself, not by the parser.
The error message you see, target of assignment expands to non-language object, is triggered by the assignment function, precisely because your target evaluates to a string.
We can fix that building up a call that has the symbols you want in the right places. The most "brute force" method is to put everything inside a string and use parse:
parse(text=paste('N',i," -> ",'z$x',i,sep=""))
Another way to get there is to use substitute:
substitute(x -> y, list(x=as.symbol(paste("N",i,sep="")), y=substitute(z$w, list(w=paste("x",i,sep="")))))
the inner substitute creates the calls z$x1, z$x2 etc. The outer substitute puts this call as the taget of the assignment, and the symbols N1, N2 etc as the values.
parse results in an expression, and substitute in a call. Both can be passed to eval to get the same result.
Just one final note: I repeat that all this is intended as a didactic example, to help understanding the inner workings of the language, but it is far from good programming practice to use parse and substitute, except when there is really no alternative.
A data.frame is a named list. It usually good practice, and idiomatically R-ish not to have lots of objects in the global environment, but to have related (or similar) objects in lists and to use lapply etc.
You could use list2env to multiassign the named elements of your list (the columns in your data.frame) to the global environment
DD <- data.frame(x = 1:3, y = letters[1:3], z = 3:1)
list2env(DD, envir = parent.frame())
## <environment: R_GlobalEnv>
## ta da, x, y and z now exist within the global environment
x
## [1] 1 2 3
y
## [1] a b c
## Levels: a b c
z
## [1] 3 2 1
I am not exactly sure what you are trying to accomplish. But here is a guess:
### Create a data.frame using the alphabet
data <- data.frame(x = 'a', y = 'b', z = 'c')
### Create a numerical index corresponding to the letter position in the alphabet
index <- which(tolower(letters[1:26]) == data[1, ])
### Use an 'lapply' to apply a function to every element in 'index'; creates a list
val <- lapply(index, function(x) {
paste('N', x, sep = '')
})
### Assign names to our list
names(val) <- names(data)
### Observe the result
val$x