Overlaying points and controlling size with ggplot2 - r

I want to plot some point estimates with a couple of interval estimates around them, and then to superimpose the true point values using a different color and size, with a legend for the color.
I've tried lots of things. If I just use a new call to geom_point, I can't figure out how to add a legend. Therefore, my current approach resorts to stacking the data on top of itself, which is clumsy. Even then, the graph comes out wrong with big blue points for the True values, with the desired orange points on top of them.
I'd appreciate any help I can get.
nms <- c("2.5%","25%","50%","75%","97.5%","dose","truep")
a <- c(9.00614679684893e- 44,0.000123271800672435,0.0339603711049475,0.187721170170911,0.67452033450121,5,0.040752445325937)
b <- c(1.59502878028266e-25,0.00328588588499889,0.0738203422543555,0.25210200886225,0.714843425007051,10,0.0885844107052267)
cc <- c(1.41975723605948e-14,0.0184599181547097,0.118284929584256,0.311068595276067,0.74339745948793,15,0.141941915501108)
d <- c(0.0311851190805834,0.154722028150561,0.299318020818234,0.50887634580605,0.838779816278485,25,0.359181624981881)
e <- c(0.0529617924263383,0.289588386297245,0.566777817134668,0.883959271416755,0.999999999999317,40,0.680133380561602)
f <- c(0.0598904847882839,0.327655201251564,0.640100529843672,0.950060245074853,1,50,0.768120635812406)
g <- c(0.0641613025760661,0.355626055560067,0.686504841650593,0.978023943968809,1,60,0.823805809980712)
p <- as.data.frame(t(data.frame(a, b, cc, d, e, f, g)))
names(p) <- nms
# Faff duplicating data
p$truep <- 1.2 * p$truep
p2 <- p
p2[, 1:5] <- p$truep # truep is known, so there are no intervals
p3 <- rbind(p2, p)
p3$wh <- rep((c(2, 3)), each=nrow(p))
p3$col <- rep(c("orange", "blue"), each=nrow(p))
ggplot(p3, aes(dose, `50%`)) +
geom_point(aes(size=wh, color=col)) +
scale_size(range=c(5, 7), guide="none") +
scale_color_manual(name="", labels=c("Prior", "True"), values=c("blue", "orange")) +
geom_pointrange(aes(ymin=`2.5%`, ymax=`97.5%`, x=dose), color="blue") +
geom_pointrange(aes(ymin=`25%`, ymax=`75%`, x=dose), color="blue", size=2) +
geom_point(aes(dose, truep), color="orange") +
theme(axis.text.x=element_text(size=12), axis.title.x=element_text(size=14),
axis.text.y=element_text(size=12), axis.title.y=element_text(size=14),
legend.text=element_text(size=12))
R 3.3.1, ggplot2_2.1.1
Thanks,
Harry

I found a solution by splitting the dataset in two parts:
library(dplyr)
priors <- p%>%
mutate(datatype = 'Prior')
truevals <- p%>%
select(dose, truep)%>%
mutate(datatype = 'True')
ggplot(truevals, aes(x = dose, y = truep, colour = datatype))+
geom_pointrange(data = priors, aes(ymin=`25%`, ymax=`75%`, y = `50%`), size=1.5) +
geom_pointrange(data = priors, aes(ymin=`2.5%`, ymax=`97.5%`, y = `50%`))+
geom_point()+
scale_color_manual(name="", values=c("Prior" = "blue", "True" = "orange")) +
theme(axis.text.x=element_text(size=12), axis.title.x=element_text(size=14),
axis.text.y=element_text(size=12), axis.title.y=element_text(size=14),
legend.text=element_text(size=12))
First we plot the two pointranges based on the dataset with priors. Then the actual values. By adding a row with the datatype to both datasets we can add the legend. The result is this graph:

For the method ggplot2::geom_point() there is a show.legend attribute which is NA by default so setting this to TRUE should help.
You can add a legend using the labels attribute as follows:
ggplot2::scale_fill_manual(values = c("red", "black",
labels = c("Number of people",
"Number of birds"))
You are already doing this with labels=c("Prior", "True")
You can also change the look of the legend with:
ggplot2::theme(legend.position = "bottom",
legend.text = ggplot2::element_text(size = 22),
legend.box = "horizontal",
legend.key = ggplot2::element_blank())

Related

R: Changing the Color of Overlapping Points

I am working with the R programming language. I made the following graph that shows a scatterplot between points of two different colors :
library(ggplot2)
a = rnorm(10000,10,10)
b = rnorm(10000, 10, 10)
c = as.factor("red")
data_1 = data.frame(a,b,c)
a = rnorm(10000,7,5)
b = rnorm(10000, 7, 5)
c = as.factor("blue")
data_2 = data.frame(a,b,c)
final = rbind(data_1, data_2)
my_plot = ggplot(final, aes(x=a, y=b, col = c)) + geom_point() + theme(legend.position="top") + ggtitle("My Plot")
My Question: Is there a way to "change the colors of overlapping points"?
Here is what I tried so far:
1) I found the following question (Visualizing two or more data points where they overlap (ggplot R)) and tried the strategy suggested:
linecolors <- c("#714C02", "#01587A", "#024E37")
fillcolors <- c("#9D6C06", "#077DAA", "#026D4E")
# partially transparent points by setting `alpha = 0.5`
ggplot(final, aes(a,b, colour = c, fill = c)) +
geom_point(alpha = 0.5) +
scale_color_manual(values=linecolors) +
scale_fill_manual(values=fillcolors) +
theme_bw()
This shows the two different colors along with the overlap, but it is quite dark and still not clear. Is there a way to pick better colors/resolutions for this?
2) I found the following link which shows how to make color gradients for continuous variables : https://drsimonj.svbtle.com/pretty-scatter-plots-with-ggplot2 - but I have discrete colors and I do not know how to apply this
3) I found this question over here (Any way to make plot points in scatterplot more transparent in R?) which shows to do this with the base R plot, but not with ggplot2:
addTrans <- function(color,trans)
{
# This function adds transparancy to a color.
# Define transparancy with an integer between 0 and 255
# 0 being fully transparant and 255 being fully visable
# Works with either color and trans a vector of equal length,
# or one of the two of length 1.
if (length(color)!=length(trans)&!any(c(length(color),length(trans))==1)) stop("Vector lengths not correct")
if (length(color)==1 & length(trans)>1) color <- rep(color,length(trans))
if (length(trans)==1 & length(color)>1) trans <- rep(trans,length(color))
num2hex <- function(x)
{
hex <- unlist(strsplit("0123456789ABCDEF",split=""))
return(paste(hex[(x-x%%16)/16+1],hex[x%%16+1],sep=""))
}
rgb <- rbind(col2rgb(color),trans)
res <- paste("#",apply(apply(rgb,2,num2hex),2,paste,collapse=""),sep="")
return(res)
}
cols <- sample(c("red","green","pink"),100,TRUE)
# Very transparant:
plot(final$a , final$b ,col=addTrans(cols,100),pch=16,cex=1)
But this is also not able to differentiate between the two color classes that I have.
Problem: Can someone please suggest how to fix the problem with overlapping points, such that the overlap appear more visible?
Thanks!
I would use a density heatmap
ggplot(final, aes(x=a, y=b, col = c))+
stat_density_2d(aes(fill = stat(density)), geom = 'raster', contour = FALSE) +
scale_fill_viridis_c() +
coord_cartesian(expand = FALSE) +
geom_point(shape = '.', col = 'white')
or
ggplot(final, aes(x=a, y=b, col = c))+
stat_density_2d(aes(fill = stat(level)), geom = 'polygon') +
scale_fill_viridis_c(name = "density") +
geom_point(shape = '.')
or
ggplot(final, aes(x=a, y=b, col = c))+
geom_point(alpha = 0.1) +
geom_rug(alpha = 0.01)

Heatmap with condition

I work with some rnaseq data, and a need to plot a heatmap with dots at determined transcripts of genes. I can not figure out how to this with ggpplot or pheatmap. So I have to use inkscape to manually put every dot on the plot. It's exausting, and a waste of time. Bellow is the image from inkscape:
I've made the basic plot with this code:
pal <- colorRampPalette(c("blue","white","red"))
a<-pal(200)
my_sample_col <- data.frame(Condition =
c("ALZxCon","PAxCon","PSPxCon"))
rownames(my_sample_col)<- colnames(transcript.table[,1:3])
my_colour <- list(Condition = c(ALZxCon = "lightblue",PAxCon =
"pink",PSPxCon = "yellow"))
pheatmap(transcript.table[,1:3],annotation_col =
my_sample_col,annotation_colors = my_colour[1],
color=a,show_colnames = F,cellheight = 15,cex=1,cluster_rows =
F,cluster_cols = F,
fontsize_row = 10,gaps_col = c(1,2),cellwidth = 15)
Where transcript table is something like this:
log2FC(AZ) log2FC(PA) log2FC(PSP) Sig(AZ) Sig(PA) Sig(PSP)
ABCA7_ENST000002633094 -0.2 -0.3 -0.2 Not Sig FDR<0.05 FDR<0.05
ABCA7_ENST0000043319 -0.6 -0.37 -0.7 FDR<0.05 FDR<0.05 FDR<0.05
I want to generate a heatmap where the square of the transcripts with FDR < 0.05 gets a black dot. Can you guys help with this?
I'm personally not an enormous fan of functions such as pheatmap, precisely because you can't customise every detail you would want. I'll show an alternative with ggplot2.
First things first, ggplot likes data in a long format, which I would do as follows:
# Loading in your data
z <- "log2FC(AZ),log2FC(PA),log2FC(PSP),Sig(AZ),Sig(PA),Sig(PSP)
ABCA7_ENST000002633094,-0.2,-0.3,-0.2,Not Sig,FDR<0.05,FDR<0.05
ABCA7_ENST0000043319,-0.6,-0.37,-0.7,FDR<0.05,FDR<0.05,FDR<0.05"
tab <- read.table(text=z, header = T, sep = ",")
# Converting to long format
lfc <- tab[,1:3]
pval <- tab[,4:6]
colnames(lfc) <- colnames(pval) <- c("AZ", "PA", "PSP")
lfc <- reshape2::melt(as.matrix(lfc))
pval <- reshape2::melt(as.matrix(pval))
df <- cbind(lfc, pval = pval$value)
Which will get us our main ingredients for the heatmap and the significance dots, but we would need a little extra data.frame for some annotation:
anno <- data.frame(x = levels(df$Var2),
y = "Condition")
Now the trick in getting this annotation to work nicely with the heatmap is a package called ggnewscale, which will allow us to set both a continuous fill for the heatmap and a discrete fill for the annotation. What remains is to make the actual plot, wherein I've tried to conserve some aspects of the pheatmap function in your example.
library(ggnewscale)
ggplot(df, aes(Var2, Var1)) +
# Important for ggnewscale is to specify a fill in the layer/geom itself
geom_tile(aes(fill = value),
width = 0.9, colour = "grey50") +
geom_point(data = df[df$pval == "FDR<0.05",]) +
scale_fill_gradientn(colours = c("blue", "white", "red"),
limits = c(-1,1)*max(abs(df$value)),
name = expression(atop("Log"[2]*" Fold","Change"))) +
# Set new scale fill after you've specified the scale for the heatmap
new_scale_fill() +
geom_tile(data = anno, aes(x, y, fill = x),
width = 0.9, height = 0.8, colour = "grey50") +
scale_fill_discrete(name = "Condition") +
scale_x_discrete(name = "", expand = c(0,0)) +
scale_y_discrete(name = "", expand = c(0,0),
limits = c(levels(df$Var1), "Condition"),
position = "right") +
coord_equal() +
theme(panel.background = element_blank(),
axis.ticks = element_blank(),
axis.text.x = element_blank(),
axis.text.y = element_text(face = c(rep("plain", nlevels(df$Var1)), "bold")))
Which looks like this:
Mix and match the ggplot code as you please.

ggplot pie charts / bar graph, force a legend [duplicate]

I'm struggling with ggplot (I always do). There are a number of very similar questions about forcing ggplot to include zero value categories in legends - here and here (for example). BUT I (think I) have a slightly different requirement to which all my mucking about with scale_x_discrete and scale_fill_manual has not helped.
Requirement: As you can see; the right-hand plot has no data in the TM=5 category - so is missing. What I need is for that right plot to have category 5 shown on the axis but obviously with no points or box.
Current Plot Script:
#data
plotData <- data.frame("TM" = c(3,2,3,3,3,4,3,2,3,3,4,3,4,3,2,3,2,2,3,2,3,3,3,2,3,1,3,2,2,4,4,3,2,3,4,2,3),
"Score" = c(5,4,4,4,3,5,5,5,5,5,5,3,5,5,4,4,5,4,5,4,5,4,5,4,4,4,4,4,5,4,4,5,3,5,5,5,5))
#vars
xTitle <- bquote("T"["M"])
v.I <- plotData$TM
depVar <- plotData$Score
#plot
p <- ggplot(plotData, aes_string(x=v.I,y=depVar,color=v.I)) +
geom_point() +
geom_jitter(alpha=0.8, position = position_jitter(width = 0.2, height = 0.2)) +
geom_boxplot(width=0.75,alpha=0.5,aes_string(group=v.I)) +
theme_bw() +
labs(x=xTitle) +
labs(y=NULL) +
theme(legend.position='none',
axis.text=element_text(size=10, face="bold"),
axis.title=element_text(size=16))
Attempted Solutions:
drop=False to scales (suggested by #Jarretinha here) totally borks margins and x-axis labels
> plot + scale_x_discrete(drop=FALSE) + scale_fill_manual(drop=FALSE)
Following logic from here and manually setting the labels in scale_fill_manual does nothing and results in the same right-hand plot from example above.
> p + scale_fill_manual(values = c("red", "blue", "green", "purple", "pink"),
labels = c("Cat1", "Cat2", "Cat3", "Cat4", "Cat5"),
drop=FALSE)
Playing with this logic and trying something with scale_x_discrete results in a change to category names on x-axis but the fifth is still missing AND the margins (as attempt 1) are borked again. BUT apparent that scale_x_discrete is important and NOT the whole answer
> p + scale_x_discrete(limits = c("Cat1", "Cat2", "Cat3", "Cat4", "Cat5"), drop=FALSE)
ANSWER for above example courtesy of input from #Bouncyball & #aosmith
#data
plotData <- data.frame("TM" = c(3,2,3,3,3,4,3,2,3,3,4,3,4,3,2,3,2,2,3,2,3,3,3,2,3,1,3,2,2,4,4,3,2,3,4,2,3),
"Score" = c(5,4,4,4,3,5,5,5,5,5,5,3,5,5,4,4,5,4,5,4,5,4,5,4,4,4,4,4,5,4,4,5,3,5,5,5,5))
plotData$TM <- factor(plotData$TM, levels=1:5) # add correct (desired number of factors to input data)
#vars
xTitle <- bquote("T"["M"])
v.I <- plotData$TM
depVar <- plotData$Score
myPalette <- c('#5c9bd4','#a5a5a4','#4770b6','#275f92','#646464','#002060')
#plot
ggplot(plotData, aes_string(x=v.I,y=depVar,color=v.I)) +
geom_jitter(alpha=0.8, position = position_jitter(width = 0.2, height = 0.2)) +
geom_boxplot(width=0.75,alpha=0.5,aes_string(group=v.I)) +
scale_colour_manual(values = myPalette, drop=F) + # new line added here
scale_x_discrete(drop=F) + # new line added here
theme_bw() +
labs(x=xTitle) +
labs(y=NULL) +
theme(legend.position='none',
axis.text=element_text(size=10, face="bold"),
axis.title=element_text(size=16))
Here's a workaround you could use:
# generate dummy data
set.seed(123)
df1 <- data.frame(lets = sample(letters[1:4], 20, replace = T),
y = rnorm(20), stringsAsFactors = FALSE)
# define factor, including the missing category as a level
df1$lets <- factor(df1$lets, levels = letters[1:5])
# make plot
ggplot(df1, aes(x = lets, y = y))+
geom_boxplot(aes(fill = lets))+
geom_point(data = NULL, aes(x = 'e', y = 0), pch = NA)+
scale_fill_brewer(drop = F, palette = 'Set1')+
theme_bw()
Basically, we plot an "empty" point (i.e. pch = NA) so that the category shows up on the x-axis, but has no visible geom associated with it. We also define our discrete variable, lets as a factor with five levels when only four are present in the data.frame. The missing category is the letter e.
NB: You'll have to adjust the positioning of this "empty" point so that it doesn't skew your y axis.
Otherwise, you could use the result from this answer to avoid having to plot an "empty" point.
# generate dummy data
set.seed(123)
df1 <- data.frame(lets = sample(letters[1:4], 20, replace = T),
y = rnorm(20), stringsAsFactors = FALSE)
# define factor, including the missing category as a level
df1$lets <- factor(df1$lets, levels = letters[1:5])
# make plot
ggplot(df1, aes(x = lets, y = y)) +
geom_boxplot(aes(fill = lets)) +
scale_x_discrete(drop = F) +
scale_fill_brewer(drop = F, palette = 'Set1') +
theme_bw()

Add a legend for geom_polygon

I'm trying to produce a scatter plot with geom_point where the points are circumscribed by a smoothed polygon, with geom_polygon.
Here's my point data:
set.seed(1)
df <- data.frame(x=c(rnorm(30,-0.1,0.1),rnorm(30,0,0.1),rnorm(30,0.1,0.1)),y=c(rnorm(30,-1,0.1),rnorm(30,0,0.1),rnorm(30,1,0.1)),val=rnorm(90),cluster=c(rep(1,30),rep(2,30),rep(3,30)),stringsAsFactors=F)
I color each point according the an interval that df$val is in. Here's the interval data:
intervals.df <- data.frame(interval=c("(-3,-2]","(-2,-0.999]","(-0.999,0]","(0,1.96]","(1.96,3.91]","(3.91,5.87]","not expressed"),
start=c(-3,-2,-0.999,0,1.96,3.91,NA),end=c(-2,-0.999,0,1.96,3.91,5.87,NA),
col=c("#2f3b61","#436CE8","#E0E0FF","#7d4343","#C74747","#EBCCD6","#D3D3D3"),stringsAsFactors=F)
Assigning colors and intervals to the points:
df <- cbind(df,do.call(rbind,lapply(df$val,function(x){
if(is.na(x)){
return(data.frame(col=intervals.df$col[nrow(intervals.df)],interval=intervals.df$interval[nrow(intervals.df)],stringsAsFactors=F))
} else{
idx <- which(intervals.df$start <= x & intervals.df$end >= x)
return(data.frame(col=intervals.df$col[idx],interval=intervals.df$interval[idx],stringsAsFactors=F))
}
})))
Preparing the colors for the leged which will show each interval:
df$interval <- factor(df$interval,levels=intervals.df$interval)
colors <- intervals.df$col
names(colors) <- intervals.df$interval
Here's where I constructed the smoothed polygons (using a function courtesy of this link):
clusters <- sort(unique(df$cluster))
cluster.cols <- c("#ff00ff","#088163","#ccbfa5")
splinePolygon <- function(xy,vertices,k=3, ...)
{
# Assert: xy is an n by 2 matrix with n >= k.
# Wrap k vertices around each end.
n <- dim(xy)[1]
if (k >= 1) {
data <- rbind(xy[(n-k+1):n,], xy, xy[1:k, ])
} else {
data <- xy
}
# Spline the x and y coordinates.
data.spline <- spline(1:(n+2*k), data[,1], n=vertices, ...)
x <- data.spline$x
x1 <- data.spline$y
x2 <- spline(1:(n+2*k), data[,2], n=vertices, ...)$y
# Retain only the middle part.
cbind(x1, x2)[k < x & x <= n+k, ]
}
library(data.table)
hulls.df <- do.call(rbind,lapply(1:length(clusters),function(l){
dt <- data.table(df[which(df$cluster==clusters[l]),])
hull <- dt[, .SD[chull(x,y)]]
spline.hull <- splinePolygon(cbind(hull$x,hull$y),100)
return(data.frame(x=spline.hull[,1],y=spline.hull[,2],val=NA,cluster=clusters[l],col=cluster.cols[l],interval=NA,stringsAsFactors=F))
}))
hulls.df$cluster <- factor(hulls.df$cluster,levels=clusters)
And here's my ggplot command:
library(ggplot2)
p <- ggplot(df,aes(x=x,y=y,colour=interval))+geom_point(cex=2,shape=1,stroke=1)+labs(x="X", y="Y")+theme_bw()+theme(legend.key=element_blank(),panel.border=element_blank(),strip.background=element_blank())+scale_color_manual(drop=FALSE,values=colors,name="DE")
p <- p+geom_polygon(data=hulls.df,aes(x=x,y=y,group=cluster),color=hulls.df$col,fill=NA)
which produces:
My question is how do I add a legend for the polygon under the legend for the points? I want it to a legend with 3 lines colored according to the cluster colors and the corresponding cluster number beside each line?
Slightly different output, only changing the last line of your code, it may solve your purpose:
p+geom_polygon(data=hulls.df,aes(x=x,y=y,group=cluster, fill=cluster),alpha=0.1)
Say, you want to add a legend of the_factor. My basic idea is,
(1) put the_factor into mapping by using unused aes arguments; aes(xx = the_factor)
(2) if (1) affects something, delete the effect by using scale_xx_manual()
(3) modify the legend by using guides(xx = guide_legend(override.aes = list()))
In your case, aes(fill) and aes(alpha) are unused. The former is better to do it because of no effect. So I used aes(fill=as.factor(cluster)).
p <- ggplot(df,aes(x=x,y=y,colour=interval, fill=as.factor(cluster))) + # add aes(fill=...)
geom_point(cex=2, shape=1, stroke=1) +
labs(x="X", y="Y",fill="cluster") + # add fill="cluster"
theme_bw() + theme(legend.key=element_blank(),panel.border=element_blank(),strip.background=element_blank()) + scale_color_manual(drop=FALSE,values=colors,name="DE") +
guides(fill = guide_legend(override.aes = list(colour = cluster.cols, pch=0))) # add
p <- p+geom_polygon(data=hulls.df,aes(x=x,y=y,group=cluster), color=hulls.df$col,fill=NA)
Of course, you can make the same graph by using aes(alpha = the_factor)). Because it has influence, you need to control it by using scale_alpha_manual().
g <- ggplot(df, aes(x=x,y=y,colour=interval)) +
geom_point(cex=2, shape=1, stroke=1, aes(alpha=as.factor(cluster))) + # add aes(alpha)
labs(x="X", y="Y",alpha="cluster") + # add alpha="cluster"
theme_bw() + theme(legend.key=element_blank(),panel.border=element_blank(),strip.background=element_blank()) + scale_color_manual(drop=FALSE,values=colors,name="DE") +
scale_alpha_manual(values=c(1,1,1)) + # add
guides(alpha = guide_legend(override.aes = list(colour = cluster.cols, pch=0))) # add
g <- p+geom_polygon(data=hulls.df,aes(x=x,y=y,group=cluster), color=hulls.df$col,fill=NA)
What you are asking for is two colour scales. My understanding is that this is not possible. But you can give the impression of having two colour scales with a bit of a cheat and using the filled symbols (shapes 21 to 25).
p <- ggplot(df, aes(x = x, y = y, fill = interval)) +
geom_point(cex = 2, shape = 21, stroke = 1, colour = NA)+
labs(x = "X", y = "Y") +
theme_bw() +
theme(legend.key = element_blank(), panel.border = element_blank(), strip.background = element_blank()) +
scale_fill_manual(drop=FALSE, values=colors, name="DE") +
geom_polygon(data = hulls.df, aes(x = x, y = y, colour = cluster), fill = NA) +
scale_colour_manual(values = cluster.cols)
p
Alternatively, use a filled polygon with a low alpha
p <- ggplot(df,aes(x=x,y=y,colour=interval))+
geom_point(cex=2,shape=1,stroke=1)+
labs(x="X", y="Y")+
theme_bw() +
theme(legend.key = element_blank(),panel.border=element_blank(), strip.background=element_blank()) +
scale_color_manual(drop=FALSE,values=colors,name="DE", guide = guide_legend(override.aes = list(fill = NA))) +
geom_polygon(data=hulls.df,aes(x=x,y=y,group=cluster, fill = cluster), alpha = 0.2, show.legend = TRUE) +
scale_fill_manual(values = cluster.cols)
p
But this might make the point colours difficult to see.

Change the colour palette in histogram

I am trying to change the colours of my histogram, but not sure how to do it, that's my code:
qplot(user, count, data=count_group, geom="histogram", fill=group,
xlab = "users", ylab="count",
main="Users")+
opts(axis.text.x=theme_text(angle=90, hjust=0, size=7))
here is the histogram I get, but the default colours are too bright,
I would like to use colours like this
I tried to add the line, but it didnt work.
scale_fill_brewer(palette = palette)
If you want to use the Brewer Set1 with that many groups, you could do something like this:
library(ggplot2)
count_group <- data.frame(user=factor(rep(1:50, 2)),
count=sample(100, 100, replace=T),
group=factor(rep(LETTERS[1:20], 5)))
library(RColorBrewer)
cols <- colorRampPalette(brewer.pal(9, "Set1"))
ngroups <- length(unique(count_group$group))
qplot(user, count, data=count_group, geom="histogram", fill=group,
xlab = "users", ylab="count") +
opts(axis.text.x=theme_text(angle=90, hjust=0, size=7)) +
scale_fill_manual(values = cols(ngroups))
EDIT
You can create and use multiple colorRampPalettes, e.g. to assign blues to groups A to J and reds to groups K to T:
blues <- colorRampPalette(c('dark blue', 'light blue'))
reds <- colorRampPalette(c('pink', 'dark red'))
qplot(user, count, data=count_group, geom="histogram", fill=group,
xlab = "users", ylab="count") +
opts(axis.text.x=theme_text(angle=90, hjust=0, size=7)) +
scale_fill_manual(values = c(blues(10), reds(10)))
# blues(10) and reds(10) because you want blues for the first ten
# groups, and reds thereafter. Each of these functions are equivalent
# to providing vectors containing ten hex colors representing a gradient
# of blues and a gradient of reds.
An update on jbaums's answer. As far as I can make out, with the new ggplot2 (as of March 2014), the following syntax is available:
p <- qplot(user, count,
data = count_group,
geom = "histogram",
stat = "bin2d",
fill = group,
xlab = "users",
ylab = "count"
)
p <- p + theme(axis.text.x = element_text(angle = 90, hjust = 0, size = 7))
p <- p + scale_fill_manual(values = cols(ngroups))
p
This was a little long for a comment, but it's not a full answer, the rest of the code is as given by jbaums, who must be thanked!

Resources