Use math symbols in panel titles for stratigraphic plot - r

I want to include math symbols in the panel titles for this stratigraphic plot:
library(analogue)
data(V12.122)
Depths <- as.numeric(rownames(V12.122))
names(V12.122)
(plt <- Stratiplot(Depths ~ O.univ + G.ruber + G.tenel + G.pacR,
data = V12.122,
type = c("h","l","g"),
zones = 400))
plt
For example, I want to have this text in place of "O.univ" etc.:
I used this code to make that text:
plot(1, type="n", axes=FALSE, ann=FALSE)
title(line = -1, main = expression(phantom()^14*C~years~BP))
title(line = -3, main = expression(delta^18*O))
title(line = -5, main = expression(paste("TP ", mu,"g l"^-1)))
title(line = -10, main = expression("very long title \n with \n line breaks"))
But if I try to update the colnames of the data frame passed to Stratiplot, the code is not parsed, and we do not get the correct text formatting:
V12.122 <- V12.122[, 1:4]
names(V12.122)[1] <- expression(phantom()^14*C~years~BP)
names(V12.122)[2] <- expression(delta^18*O)
names(V12.122)[3] <- expression(paste("TP ", mu,"g l"^-1))
(plt <- Stratiplot(Depths ~ .,
data = V12.122,
type = c("h","l","g"),
zones = 400))
plt
How can I get Stratiplot to parse the expressions in the colnames and format them correctly in the plot?
I've tried looking through str(plt) to see where the panel titles are stored, but no success:
text <- expression(phantom()^14*C~years~BP)
plt$condlevels$ind[1] <- text
names(plt$packet.sizes)[1] <- text
names(plt$par.settings$layout.widths$panel)[1] <- text

You can't actually do this in the current release of analogue; the function is doing too much messing around with data for the expressions to remain unevaluated prior to plotting. I could probably figure this out to allow expressions as the names of the data argument object, but it is easier to just allow users to pass a vector of labels that they want for the variables.
This is now implemented in the development version of the package on github, and I'll push this to CRAN early next week.
This change implements a new argument labelValues which takes a vector of labels for use in labelling the top axis. This can be a vector of expressions.
Here is an illustration of the usage:
library("analogue")
set.seed(1)
df <- setNames(data.frame(matrix(rnorm(200 * 3), ncol = 3)),
c("d13C", "d15N", "d18O"))
df <- transform(df, Age = 1:200)
exprs <- expression(delta^{13}*C, # label for 1st variable
delta^{15}*N, # label for 2nd variable
delta^{18}*O) # label for 3rd variable
Stratiplot(Age ~ ., data = df, labelValues = exprs, varTypes = "absolute", type = "h")
which produces
Note that this is just a first pass; I'm pretty sure I haven't accounted for any reordering that goes on with sort and svar etc. if they are used.

Never used lattice plots, but I thought a chance to learn something should be worth while. Took too long to figure out.
text <- "c( expression(phantom()^14*C~years~BP),expression(delta^18*O))"
strip = strip.custom(factor.levels=eval(parse(text=text)))
plt <- Stratiplot(Depths ~ .,
data = V12.122[, 1:4],
type = c("h","l","g"),
zones = 400,
strip = strip)
Hope this gets you started.

Related

I can't get my plots to a single grid please help correct my code

I have 11 plots and used a looping function to plot them see my code below. However, I can't get them to fit in just 1 page or less. The plots are actually too big. I am using R software and writing my work in RMarkdown. I have spent almost an entire week trying to resolve this.
group_by(Firm_category) %>%
doo(
~ggboxplot(
data =., x = "Means.type", y = "means",
fill ="grey", palette = "npg", legend = "none",
ggtheme = theme_pubr()
),
result = "plots"
)
graph3
# Add statistical tests to each corresponding plot
Firm_category <- graph3$Firm_category
xx <- for(i in 1:length(Firm_category)){
graph3.i <- graph3$plots[[i]] +
labs(title = Firm_category[i]) +
stat_pvalue_manual(stat.test[i, ], label = "p.adj.signif")
print(graph3.i)
}
#output3.long data sample below as comments
#Firm_category billmonth Means.type means
#Agric 1 Before 38.4444
#Agric 1 After 51.9
Complete data is on my github: https://github.com/Fridahnyakundi/Descriptives-in-R/blob/master/Output3.csv
This code prints all the graphs but in like 4 pages. I want to group them into a grid. I have tried to add all these codes below just before my last curly bracket and none is working, please help me out.
library(cowplot)
print(plot_grid(plotlist = graph3.i[1:11], nrow = 4, ncol = 3))
library(ggpubr)
print(ggarrange(graph3.i[1:11], nrow = 4, ncol = 3))
I tried the gridExtra command as well (they all seem to do the same thing). I am the one with a mistake and I guess it has to do with my list. I read a lot of similar work here, some suggested
dev.new()
dev.off()
I still didn't get what they do. But adding either of them caused my code to stop.
I tried defining my 'for' loop function say call it 'XX', then later call it to make a list of graph but it returned NULL output.
I have tried defining an empty list (as I read in some answers here) then counting them to make a list that can be printed but I got so many errors.
I have done this for almost 3 days and will appreciate your help in resolving this.
Thanks!
I tried to complete your code ... and this works (but I don't have your 'stat.test' object). Basically, I added a graph3.i <- list() and replaced graph3.i in the loop ..
Is it what you wanted to do ?
library(magrittr)
library(dplyr)
library(rstatix)
library(ggplot2)
library(ggpubr)
data <- read.csv(url('http://raw.githubusercontent.com/Fridahnyakundi/Descriptives-in-R/master/Output3.csv'))
graph3 <- data %>% group_by(Firm_category) %>%
doo(
~ggboxplot(
data =., x = "Means.type", y = "means",
fill ="grey", palette = "npg", legend = "none",
ggtheme = theme_pubr()
),
result = "plots"
)
graph3
# Add statistical tests to each corresponding plot
graph3.i <- list()
Firm_category <- graph3$Firm_category
xx <- for(i in 1:length(Firm_category)){
graph3.i[[i]] <- graph3$plots[[i]] +
labs(title = Firm_category[i]) # +
# stat_pvalue_manual(stat.test[i, ], label = "p.adj.signif")
print(graph3.i)
}
library(cowplot)
print(plot_grid(plotlist = graph3.i[1:11], nrow = 4, ncol = 3))

Weird characters appearing in the plot legend when using DoHeatmap

I was using Seurat to analyse single cell RNA-seq data and I managed to draw a heatmap plot with DoHeatmap() after clustering and marker selection, but got a bunch of random characters appearing in the legend. They are random characters as they will change every time you run the code. I was worrying over it's something related to my own dataset, so I then tried the test Seurat object 'ifnb' but still got the same issue (see the red oval in the example plot).
example plot
I also tried importing the Seurat object in R in the terminal (via readRDS) and ran the plotting function, but got the same issue there, so it's not a Rstudio thing.
Here are the codes I ran:
'''
library(Seurat)
library(SeuratData)
library(patchwork)
InstallData("ifnb")
LoadData("ifnb")
ifnb.list <- SplitObject(ifnb, split.by = "stim")
ifnb.list <- lapply(X = ifnb.list, FUN = function(x) {
x <- NormalizeData(x)
x <- FindVariableFeatures(x, selection.method = "vst", nfeatures = 2000)
})
features <- SelectIntegrationFeatures(object.list = ifnb.list)
immune.anchors <- FindIntegrationAnchors(object.list = ifnb.list, anchor.features = features)
immune.combined <- IntegrateData(anchorset = immune.anchors)
immune.combined <- ScaleData(immune.combined, verbose = FALSE)
immune.combined <- RunPCA(immune.combined, npcs = 30, verbose = FALSE)
immune.combined <- RunUMAP(immune.combined, reduction = "pca", dims = 1:30)
immune.combined <- FindNeighbors(immune.combined, reduction = "pca", dims = 1:30)
immune.combined <- FindClusters(immune.combined, resolution = 0.5)
DefaultAssay(immune.combined) <- 'RNA'
immune_markers <- FindAllMarkers(immune.combined, latent.vars = "stim", test.use = "MAST", assay = 'RNA')
immune_markers %>%
group_by(cluster) %>%
top_n(n = 10, wt = avg_log2FC) -> top10_immune
DoHeatmap(immune.combined, slot = 'data',features = top10_immune$gene, group.by = 'stim', assay = 'RNA')
'''
Does anyone have any idea how to solve this issue other than reinstalling everything?
I have been having the same issue myself and while I have solved it by not needing the legend, I think you could use this approach and use a similar solution:
DoHeatmap(immune.combined, slot = 'data',features = top10_immune$gene, group.by = 'stim', assay = 'RNA') +
scale_color_manual(
values = my_colors,
limits = c('CTRL', 'STIM'))
Let me know if this works! It doesn't solve the source of the odd text values but it does the job! If you haven't already, I would recommend creating a forum question on the Seurat forums to see where these characters are coming from!
When I use seurat4.0, I met the same problem.
While I loaded 4.1, it disappeared

R baseline package saving plots in a loop

I'm trying to optimize the parameters for baseline in the R baseline package by changing each parameters in a loop and comparing plots to determine which parameters give me the best baseline.
I currently have the code written so that the loop produces each plot, but I'm having trouble with getting the plot saved as the class of each object I'm creating is a baseline package-specific (which I'm suspecting is the problem here).
foo <- data.frame(Date=seq.Date(as.Date("1957-01-01"), by = "day",
length.out = ncol(milk$spectra)),
Visits=milk$spectra[1,],
Old_baseline_visits=milk$spectra[1,], row.names = NULL)
foo.t <- t(foo$Visits)
#the lines above were copied from https://stackoverflow.com/questions/37346967/r-packagebaseline-application-to-sample-dataset to make a reproducible dataset
df <- expand.grid(lambda=seq(1,10,1), p=seq(0.01,0.1,0.01))
baselinediff <- list()
for(i in 1:nrow(df)){
thislambda <- df[i,]$lambda
thisp <- df[i,]$p
thisplot <- baseline(foo.t, lambda=thislambda, p=thisp, maxit=20, method='als')
print(paste0("lambda = ", thislambda))
print(paste0("p = ", thisp))
print(paste0("index = ", i))
baselinediff[[i]] <- plot(thisplot)
jpeg(file = paste(baselinediff[[i]], '.jpeg', sep = ''))
dev.off()
}
I know that I would be able to extract corrected spectra using baseline.als but I just want to save the plot images with the red baseline so that I can see how well the baselines are getting drawn. Any baseline users out there that can help?
I suggest you change your loop in the following way:
for(i in 1:nrow(df)){
thislambda <- df[i,]$lambda
thisp <- df[i,]$p
thisplot <- baseline(foo.t, lambda=thislambda, p=thisp, maxit=20, method='als')
print(paste0("lambda = ", thislambda))
print(paste0("p = ", thisp))
print(paste0("index = ", i))
baselinediff[[i]] <- thisplot
jpeg(file = paste('baseline', i, '.jpeg', sep = ''))
plot(baselinediff[[i]])
dev.off()
}
Note that this does not try to capture the already plotted element (thisplot) inside of the list. Instead, the plotting is done after you call the jpeg command. This solves your export issue. Another problem was the naming of the file. If you call baselinediff[[i]] inside of paste, you apparently end up with an error. So I switched it to a simpler name. To plot your resulting list, call:
lapply(baselinediff, plot)
If you are determined on storing the already plotted element, the capture.plotfunction from the imager package might be a good start.

Legend in multiple plot in R

According to the comments from others, this post has been separated into several
smaller questions from the previous version of this OP.
In the graph below, will you help me to (Newbie to R)
Custom legends according to the data they represent like filled for variable 1, circle points for variable 2 and line for variable 3 and their colors.
same letter size for the legend and axis-names.
The graph below is produced with the data in pdf device with following layout.
m <- matrix(c(1,2,3,3,4,5),nrow = 3,ncol = 2,byrow = TRUE)
layout(mat = m,heights = c(0.47,0.06,0.47))
par(mar=c(4,4.2,3,4.2))
#Codes for Fig A and B
...
#Margin for legend
par(mar = c(0.2,0.2,0.1,0.1))
# Code for legend
...
#Codes for Fig C and D
...
Using doubleYScale from latticeExtra and the data in the long format (see my previous answer), you can simplify the work:
No need to create a custom layout to superpose many plots
No need to create the legend manually
The idea is to create 2 separates objects and then merge them using doubleYScale. The latter will create the second axes. I hope I get your ploygon idea since it is not very clear why do you invert it in your OP.
library(latticeExtra)
obj1 <- xyplot(Variable~TimeVariable|Type,type='l',
groups=time, scales=list(x=list(relation='free'),
y=list(relation='free')),
auto.key=list(columns = 3,lines = TRUE,points=FALSE) ,
data = subset(dat.l,time !=1))
obj2 <- xyplot(Variable~TimeVariable|Type,
data = subset(dat.l,time ==1),type='l',
scales=list(x=list(alternating=2),
auto.key=list(columns = 3,lines = TRUE,points=FALSE),
y=list(relation='free')),
panel=function(x,y,...){
panel.xyplot(x,y,...)
panel.polygon(x,y,col='violetred4',border=NA,alpha=0.3)
})
doubleYScale(obj1, obj2, add.axis = TRUE,style1 = 0, style2 = 1)
Try the following:
1) For the legend part
The data can be found on https://www.dropbox.com/s/4kgq8tyvuvq22ym/stackfig1_2.csv
The code I used is as follows:
data <- read.csv("stackfig1_2.csv")
library(Hmisc)
label1=c(0,100,200,300)
plot(data$TimeVariable2C,data$Variable2C,axes=FALSE,ylab="",xlab="",xlim=c(0,24),
ylim=c(0,2.4),xaxs="i",yaxs="i",pch=19)
lines(data$TimeVariable3C,data$Variable3C)
axis(2,tick=T,at=seq(0.0,2.4,by=0.6),label= seq(0.0,2.4,by=0.6))
axis(1,tick=T,at=seq(0,24,by=6),label=seq(0,24,by=6))
mtext("(C)",side=1,outer=F,line=-10,adj=0.8)
minor.tick(nx=5,ny=5)
par(new=TRUE)
plot(data$TimeVariable1C,data$Variable1C,axes=FALSE,xlab="",ylab="",type="l",
ylim=c(800,0),xaxs="i",yaxs="i")
axis(3,xlim=c(0,24),tick=TRUE,at= seq(0,24,by=6),label=seq(0,24,by=6),col.axis="violetred4",col="violetred4")
axis(4,tick=TRUE,at= label1,label=label1,col.axis="violetred4",col="violetred4")
polygon(data$TimeVariable1C,data$Variable1C,col='violetred4',border=NA)
legend("top", legend = c("Variable A","Variable B","Variable C"), col = c("black","violetred4","black"),
ncol = 2, lwd =c("","",2),pch=c(19,15,NA),cex=1)
The output is as follows:
2) To make the font size same use the parameter cex and make it same everywhere.

Making simple R GUI with tcltk package

I'm trying to make very simple GUI for my script. In nutshell problem looks like that :
dataset is dataframe, I would like to plot one column as the time and use simple GUI for choosing next/previus column.
dataset <-data.frame(rnorm(10), rnorm(10), rnorm(10))
columnPlot <- function(dataset, i){
plot(dataset[, i])
}
how to use tcltk for calling fplot with different i's ?
Not what you asked for (not tcltkrelated), but I would advise you to have a look at the new shiny package from RStudio.
Are you particularly attached to the idea of using tcltk? I've been working on something similar using the gWidgets package and have had some success. According to it's CRAN site, "gWidgets provides a toolkit-independent API for building interactive GUIs". This package uses tcltk or GTK2 and I've been using the GTK2 portion. Here's a quick example of a GUI with a spinbutton for changing i. I also added a little fanciness to your function because you mentioned you would be plotting time series, so I made the x axis Time.
data<-data.frame(rnorm(11),rnorm(11),rnorm(11))
i = 1
fplot <- function(i, data = data){
library(ggplot2)
TimeStart <- as.Date('1/1/2012', format = '%m/%d/%Y')
plotdat <- data.frame(Value = data[ ,i], Time = seq(TimeStart,TimeStart + nrow(data) - 1, by = 1))
myplot <- ggplot(plotdat, aes(x = Time, y = Value))+
geom_line()
print(myplot)
}
library(gWidgets)
options(guiToolkit = 'RGtk2')
window <- gwindow ("Time Series Plots", visible = T)
notebook <- gnotebook (cont = window)
group1 <- ggroup(cont = notebook, label = "Choose i", horizontal=F)
ichooser <- gspinbutton(cont = group1, from = 1, to = ncol(data), by = 1, value = i, handler = function(h,...){
i <<- svalue(h$obj)})
plotbutton <- gbutton('Plot', cont = group1, handler=function(h,...){
fplot(i, data)})
graphicspane1 <- ggraphics(cont = group1)

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