Im using ggstatsplot's ggscatterstats function to calculate correlation between various clinical parameters and then plotting them. For example
here my variables are age and WBC. This is taking all the data points irrespective of the class they belong. I would like to do the same with each FAB classification that is present in my data.
dat <- merge_clinical_class_TMB %>% select(FAB,AGE,Wbc,Platelet,HB,PB_Blasts,BM_Blasts,TMB_NONSYNONYMOUS)
df2 <- dat
library(ggstatsplot)
ggscatterstats(
df2,
x = AGE,
y = Wbc,
type = "np" # try the "robust" correlation too! It might be even better here
#, marginal.type = "boxplot"
)
My dataframe looks like this
head(df2)
FAB AGE Wbc Platelet HB PB_Blasts BM_Blasts TMB_NONSYNONYMOUS
1 M4 50 17 231 10 88 52 0.3000000
2 M3 61 1 90 10 44 0 0.4333333
3 M3 30 6 114 11 82 6 0.2333333
4 M0 77 92 105 9 67 56 0.4000000
5 M1 46 29 90 9 90 81 0.5666667
6 M1 68 3 63 8 91 55 0.9000000
My data
dput(df2)
structure(list(FAB = structure(c(5L, 4L, 4L, 1L, 2L, 2L, 3L,
3L, 3L, 5L, 3L, 5L, 1L, 5L, 5L, 3L, 3L, 3L, 1L, 2L, 1L, 4L, 6L,
6L, 5L, 3L, 5L, 7L, 5L, 1L, 6L, 5L, 5L, 6L, 5L, 6L, 3L, 3L, 4L,
4L, 5L, 7L, 3L, 3L, 5L, 2L, 5L, 1L, 3L, 6L, 2L, 5L, 2L, 5L, 7L,
3L, 3L, 8L, 6L, 4L, 2L, 2L, 2L, 2L, 3L, 8L, 3L, 2L, 2L, 4L, 6L,
3L, 3L, 3L, 2L, 3L, 2L, 2L, 2L, 3L, 6L, 2L, 1L, 3L, 2L, 5L, 5L,
1L, 2L, 5L, 6L, 6L, 2L, 6L, 4L, 2L, 5L, 2L, 2L, 2L, 1L, 4L, 4L,
1L, 3L, 9L, 6L, 5L, 5L, 1L, 3L, 3L, 5L, 1L, 2L, 2L, 3L, 5L, 1L,
5L, 5L, 6L, 2L, 2L, 2L, 1L, 3L, 3L, 6L, 5L, 2L, 5L, 1L, 2L, 8L,
2L, 3L, 9L, 5L, 2L, 1L, 5L, 3L, 5L, 5L, 1L, 3L, 2L, 5L, 3L, 6L,
5L, 1L, 2L, 2L, 5L, 3L, 5L, 5L, 6L, 5L, 5L, 3L, 5L, 6L, 3L, 2L,
3L, 3L, 2L, 4L, 6L, 4L, 1L, 2L, 6L, 3L, 6L, 2L, 3L, 2L, 4L, 2L,
2L, 4L, 3L, 3L, 4L, 4L, 4L, 3L, 4L, 3L, 6L, 2L, 4L, 2L, 5L, 2L,
4L), .Label = c("M0", "M1", "M2", "M3", "M4", "M5", "M6", "M7",
"nc"), class = "factor"), AGE = c(50L, 61L, 30L, 77L, 46L, 68L,
23L, 64L, 76L, 81L, 25L, 78L, 39L, 49L, 57L, 63L, 62L, 52L, 76L,
64L, 65L, 61L, 44L, 31L, 64L, 33L, 55L, 50L, 64L, 59L, 59L, 77L,
33L, 48L, 35L, 66L, 67L, 51L, 74L, 51L, 64L, 77L, 63L, 37L, 57L,
53L, 62L, 39L, 72L, 66L, 51L, 51L, 18L, 63L, 54L, 75L, 40L, 60L,
76L, 33L, 63L, 53L, 75L, 67L, 66L, 77L, 64L, 76L, 51L, 42L, 51L,
59L, 43L, 45L, 60L, 47L, 68L, 24L, 48L, 73L, 60L, 44L, 71L, 25L,
60L, 57L, 55L, 69L, 42L, 42L, 45L, 50L, 41L, 21L, 50L, 69L, 76L,
70L, 27L, 76L, 65L, 48L, 59L, 69L, 81L, 22L, 61L, 51L, 63L, 61L,
22L, 73L, 49L, 41L, 47L, 54L, 44L, 55L, 83L, 78L, 59L, 57L, 57L,
88L, 43L, 71L, 62L, 75L, 62L, 58L, 65L, 66L, 60L, 35L, 76L, 72L,
35L, 73L, 67L, 70L, 48L, 65L, 41L, 52L, 67L, 58L, 34L, 60L, 55L,
56L, 61L, 31L, 71L, 56L, 57L, 60L, 57L, 58L, 79L, 55L, 34L, 76L,
82L, 67L, 67L, 54L, 53L, 71L, 61L, 30L, 50L, 35L, 29L, 45L, 38L,
81L, 31L, 75L, 67L, 29L, 51L, 40L, 32L, 57L, 25L, 63L, 75L, 25L,
68L, 62L, 25L, 31L, 68L, 45L, 61L, 35L, 22L, 23L, 21L, 53L),
Wbc = c(17L, 1L, 6L, 92L, 29L, 3L, 32L, 117L, 62L, 91L, 34L,
10L, 2L, 57L, 88L, 77L, 75L, 4L, 15L, 1L, 3L, 86L, 9L, 137L,
132L, 3L, 22L, 6L, 3L, 1L, 12L, 40L, 26L, 116L, 53L, 112L,
2L, 42L, 32L, 4L, 2L, 3L, 17L, 19L, 14L, 3L, 119L, 5L, 3L,
79L, 104L, 3L, 35L, 77L, 2L, 8L, 8L, 1L, 4L, 1L, 46L, 2L,
6L, 31L, 3L, 2L, 3L, 34L, 2L, 2L, 15L, 12L, 4L, 29L, 12L,
12L, 60L, 224L, 33L, 2L, 7L, 14L, 5L, 11L, 47L, 5L, 31L,
6L, 11L, 38L, 5L, 7L, 134L, 93L, 3L, 10L, 3L, 48L, 90L, 297L,
1L, 1L, 1L, 2L, 2L, 115L, 35L, 50L, 18L, 62L, 52L, 15L, 12L,
48L, 81L, 13L, 35L, 28L, 78L, 17L, 30L, 99L, 20L, 3L, 172L,
6L, 28L, 98L, 59L, 101L, 68L, 2L, 2L, 43L, 4L, 38L, 34L,
59L, 37L, 1L, 111L, 49L, 43L, 298L, 26L, 47L, 14L, 16L, 114L,
203L, 8L, 133L, 1L, 31L, 3L, 68L, 3L, 20L, 19L, 73L, 20L,
5L, 1L, 15L, 45L, 68L, 88L, 36L, 10L, 23L, 1L, 72L, 1L, 2L,
40L, 12L, 13L, 7L, 46L, 2L, 64L, NA, 5L, 103L, 8L, 1L, 3L,
16L, 29L, 1L, 99L, 2L, 6L, 2L, 3L, 2L, 115L, 27L, 8L, 1L),
Platelet = c(231L, 90L, 114L, 105L, 90L, 63L, 38L, 100L,
32L, 32L, 23L, 98L, 215L, 14L, 56L, 19L, 110L, 22L, 85L,
42L, 16L, 22L, 50L, 42L, 15L, 61L, 65L, 50L, 134L, 102L,
57L, 29L, 111L, 50L, 44L, 34L, 28L, 232L, 42L, 58L, 27L,
86L, 23L, 38L, 76L, 108L, 52L, 175L, 52L, 132L, 23L, 143L,
30L, 41L, 9L, 21L, 95L, 59L, 79L, 38L, 11L, 68L, 22L, 141L,
168L, 70L, 41L, 21L, 25L, 35L, 14L, 20L, 67L, 116L, 45L,
57L, 8L, 34L, 32L, 60L, 93L, 145L, 48L, 33L, 50L, 129L, 9L,
61L, 176L, 12L, 53L, 136L, 40L, 73L, 27L, 12L, 166L, 30L,
87L, 40L, 94L, 52L, 23L, 127L, 39L, 57L, 35L, 21L, 148L,
25L, 149L, 64L, 351L, 71L, 53L, 22L, 35L, 31L, 46L, 85L,
18L, 80L, 62L, 156L, 32L, 50L, 69L, 31L, 20L, 57L, 142L,
37L, 79L, 66L, 21L, 31L, 88L, 11L, 15L, 82L, 53L, 76L, 51L,
68L, 64L, 55L, 40L, 90L, 37L, 45L, 36L, 52L, 86L, 88L, 35L,
174L, 28L, 121L, 131L, 17L, 152L, 52L, 30L, 79L, 79L, 87L,
30L, 44L, 140L, 59L, 58L, 19L, 29L, 156L, 19L, 61L, 36L,
11L, 71L, 13L, 45L, 34L, 39L, 82L, 18L, 43L, 118L, 32L, 73L,
15L, 60L, 208L, 96L, 257L, 61L, 12L, 32L, 23L, 52L, 46L),
HB = c(10L, 10L, 11L, 9L, 9L, 8L, 7L, 10L, 10L, 11L, 11L,
10L, 10L, 8L, 10L, 13L, 11L, 9L, 9L, 8L, 9L, 12L, 8L, 6L,
10L, 7L, 8L, 9L, 11L, 12L, 11L, 10L, 10L, 9L, 8L, 10L, 9L,
13L, 9L, 8L, 12L, 9L, 12L, 9L, 9L, 9L, 11L, 10L, 11L, 12L,
12L, 11L, 9L, 10L, 9L, 9L, 10L, 9L, 10L, 9L, 8L, 9L, 9L,
10L, 12L, 10L, 10L, 8L, 10L, 9L, 11L, 11L, 11L, 8L, 9L, 9L,
9L, 6L, 10L, 10L, 9L, 9L, 8L, 9L, 9L, 7L, 9L, 11L, 12L, 10L,
9L, 10L, 12L, NA, 10L, 7L, 11L, 10L, 9L, 11L, 10L, 9L, 8L,
8L, 10L, 9L, 12L, 11L, 8L, 13L, 11L, 9L, 9L, 12L, 10L, 9L,
10L, 8L, 9L, 9L, 9L, 10L, 9L, 10L, 10L, 9L, 10L, 8L, 7L,
9L, 9L, 8L, 9L, 9L, 8L, 10L, 8L, 9L, 9L, 8L, 9L, 9L, 9L,
9L, 9L, 10L, 9L, 8L, 9L, 10L, 7L, 11L, 11L, 10L, 6L, 8L,
9L, 9L, 10L, 8L, 11L, 10L, 11L, 8L, 9L, 8L, 9L, 8L, 10L,
10L, 10L, 9L, 9L, 12L, 9L, 9L, 11L, 9L, 13L, 9L, 10L, 8L,
9L, 10L, 10L, 11L, 9L, 9L, 10L, 9L, 9L, 11L, 7L, 13L, 14L,
12L, 8L, 12L, 8L, 9L), PB_Blasts = c(88L, 44L, 82L, 67L,
90L, 91L, 59L, 60L, 48L, 98L, 53L, 40L, 75L, 81L, 90L, 57L,
46L, 67L, 74L, 61L, 99L, 73L, 74L, 83L, 72L, 33L, 35L, 70L,
85L, 61L, 95L, 80L, 71L, 83L, 90L, 90L, 50L, 64L, 51L, 93L,
95L, 75L, 80L, 52L, 61L, 72L, 65L, 83L, 45L, 32L, 85L, 73L,
86L, 82L, 30L, 48L, 47L, 58L, 78L, 100L, 81L, 82L, 40L, 89L,
70L, 47L, 80L, 73L, 62L, 88L, 57L, 70L, 40L, 56L, 86L, 37L,
90L, 77L, 75L, 37L, 94L, 86L, 97L, 72L, 87L, 40L, 52L, 60L,
68L, 40L, 95L, 81L, 92L, 90L, 90L, 42L, 37L, 84L, 77L, 99L,
83L, 65L, 79L, 82L, 46L, 94L, 71L, 39L, 62L, 95L, 55L, 11L,
51L, 42L, 77L, 72L, 39L, 69L, 75L, 70L, 75L, 52L, 91L, 33L,
87L, 55L, 72L, 76L, 85L, 79L, 79L, 81L, 50L, 81L, 33L, 88L,
34L, 90L, 69L, 32L, 92L, 90L, 47L, 75L, 30L, 59L, 57L, 62L,
54L, 60L, 89L, 82L, 90L, 90L, 64L, 89L, 43L, 58L, 58L, 97L,
71L, 91L, 53L, 75L, 85L, 67L, 86L, 70L, 43L, 86L, 74L, 87L,
0L, 0L, 86L, 53L, 63L, 41L, 76L, 45L, 85L, 0L, 94L, 6L, 91L,
0L, 2L, 93L, 85L, 82L, 56L, 40L, 48L, 0L, 14L, 90L, 71L,
51L, 91L, 42L), BM_Blasts = c(52L, 0L, 6L, 56L, 81L, 55L,
0L, 0L, 88L, 37L, 87L, 6L, 4L, 48L, 84L, 70L, 53L, 18L, 82L,
5L, 34L, 68L, 5L, 6L, 90L, 0L, 67L, 0L, 22L, 12L, 0L, 2L,
14L, 3L, 18L, 7L, 17L, 79L, 0L, 40L, 0L, 8L, 71L, 33L, 17L,
41L, 65L, 53L, 0L, 11L, 85L, 2L, 90L, 39L, 0L, 54L, 23L,
0L, 0L, 0L, 97L, 42L, 48L, 61L, 6L, 0L, 46L, 55L, 10L, 2L,
0L, 48L, 39L, 37L, 43L, 0L, 91L, 76L, 41L, 16L, 30L, 17L,
54L, 50L, 65L, 0L, 59L, 22L, 51L, 16L, 6L, 10L, 90L, 72L,
0L, 32L, 0L, 49L, 88L, 98L, 0L, 0L, 15L, 0L, 0L, 94L, 55L,
39L, 9L, 86L, 70L, 11L, 5L, 74L, 79L, 90L, 83L, 57L, 74L,
28L, 17L, 4L, 91L, 0L, 91L, 50L, 49L, 80L, 22L, 64L, 84L,
12L, 14L, 86L, 6L, 18L, 40L, 0L, 61L, 6L, 87L, 0L, 62L, 51L,
6L, 72L, 59L, 29L, 24L, 96L, 0L, 53L, 13L, 45L, 61L, 56L,
35L, 10L, 0L, 8L, 58L, 16L, 25L, 10L, 3L, 71L, 52L, 67L,
32L, 88L, 10L, 8L, 0L, 0L, 97L, 7L, 45L, 0L, 49L, 9L, 85L,
0L, 70L, 91L, 7L, 0L, 2L, 0L, 32L, 11L, 71L, 0L, 48L, 0L,
14L, 7L, 90L, 63L, 83L, 29L), TMB_NONSYNONYMOUS = c(0.3,
0.433333333333, 0.233333333333, 0.4, 0.566666666667, 0.9,
0.3, 0.133333333333, 0.4, 0.3, 0.233333333333, 0.5, 0.266666666667,
0, 0.2, 0.4, 0.266666666667, 0.333333333333, 0.4, 0.4, 0.566666666667,
0.0333333333333, 0.166666666667, 0.1, 0.166666666667, 0.266666666667,
0.3, 0.3, 0.466666666667, 0.0666666666667, 0.266666666667,
0.266666666667, 0.0333333333333, 0.1, 0.133333333333, 0.0333333333333,
0.5, 0.6, 0.0333333333333, 0.1, 0.0333333333333, 0.333333333333,
0.433333333333, 0.2, 0.466666666667, 0.2, 0.0333333333333,
0.733333333333, 0.2, 0.233333333333, 0.233333333333, 0.3,
0.133333333333, 0, 0.3, 0.333333333333, 0.333333333333, 0.266666666667,
0.533333333333, 0.2, 0.533333333333, 0.466666666667, 0.533333333333,
0.0333333333333, 0.3, 0.5, 0.333333333333, 0.266666666667,
0.5, 0.333333333333, 0.0666666666667, 0.466666666667, 0.333333333333,
0.266666666667, 0.7, 0.433333333333, 0.166666666667, 0.0666666666667,
0.233333333333, 0.5, 0.0333333333333, 0.2, 0.433333333333,
0.433333333333, 0.4, 0.233333333333, 0.0666666666667, 0.233333333333,
0.466666666667, 0.0666666666667, 0, 0.1, 0.4, 0.1, 0.2, 0.4,
0.433333333333, 0.566666666667, 0.2, 0.0333333333333, 0.533333333333,
0.566666666667, 0.3, 0.466666666667, 0.566666666667, 0.0333333333333,
0.4, 0.0666666666667, 0.633333333333, 0.4, 0.466666666667,
0.466666666667, 0.3, 0.5, 0.0333333333333, 0.333333333333,
0.333333333333, 0.266666666667, 0.366666666667, 0.666666666667,
0.333333333333, 0.533333333333, 0.466666666667, 0.6, 0.333333333333,
0.4, 0.266666666667, 0.366666666667, 0.2, 0.0333333333333,
0.266666666667, 0.3, 0.166666666667, 0.4, 0.566666666667,
0.4, 0.1, 0.1, 0.0666666666667, 0.366666666667, 0, 0.4, 0.0333333333333,
0.1, 0.0666666666667, 0.5, 0.3, 0.466666666667, 0.0333333333333,
0.4, 0.1, 0.0666666666667, 0.766666666667, 0.5, 0.466666666667,
0.333333333333, 0.4, 0.333333333333, 0.4, 0.266666666667,
0.2, 0.3, 0.7, 0.166666666667, 0.2, 0, 0.5, 0.166666666667,
0.533333333333, 0.233333333333, 0.166666666667, 0.133333333333,
0.0666666666667, 0.4, 0.333333333333, 0.133333333333, 0.4,
0.233333333333, 0.466666666667, 0.366666666667, 0.266666666667,
0.266666666667, 0.266666666667, 0.4, 0.2, 0.166666666667,
0.4, 0.333333333333, 0.166666666667, 0.266666666667, 0.1,
0.333333333333, 0.733333333333, 0.466666666667, 0.466666666667,
0.2, 0.1, 1.13333333333, 0.2, 0.3)), class = "data.frame", row.names = c(NA,
-200L))
Objective I would like to do the same with various FABI have FAB label from M0 to M7 I would like to ignore nc
So for each FAB label I would like to see the correlation for example if I have to take the M0 class then I would like to see their Age vs Wbc correlation and similarly for other FAB class as well. Is it possible to do these in ggstataplot as I don't see for correlation any such functionality there .
Simple way is I can subset them and do the same like M0 ,M1, M2 etc etc but that is a long process can I split the FAB column and pass it to the library?
I would like to know other ways to do the above and plot the same
Any help or suggestion would be appreciated
Update: We could also use the built in function see comments:
Many thanks to #Indrajeet Patil: https://indrajeetpatil.github.io/ggstatsplot/articles/web_only/ggscatterstats.html#grouped-analysis-with-grouped_ggscatterstats
To subset FAB we use filter:
## for reproducibility
set.seed(123)
## plot
grouped_ggscatterstats(
## arguments relevant for ggscatterstats
data = df2 %>% filter(as.integer(FAB)<5),
x = AGE,
y = Wbc,
grouping.var = FAB,
type = "r",
# ggtheme = ggthemes::theme_tufte(),
## arguments relevant for combine_plots
annotation.args = list(
title = "Relationship between Wbc and Age",
caption = "Source: stackoverflow"
),
plotgrid.args = list(nrow = 2, ncol = 2)
)
First answer:
We could do something like this:
write a function and pass the data frame + the column FAB value:
library(ggstatsplot)
my_function <- function(df, x){
ggscatterstats(
df %>% filter(FAB == x),
x = AGE,
y = Wbc,
type = "np" # try the "robust" correlation too! It might be even better here
#, marginal.type = "boxplot"
)
}
M0 <- my_function(df2, "M0")
M1 <- my_function(df2, "M1")
M2 <- my_function(df2, "M2")
M3 <- my_function(df2, "M3")
.
.
.
library(patchwork)
(M0 / M1 | M2 / M3)
I have a data.frame fish.test0 for which I want to grep specific variables (in varlist) matching the group column to create a sub-data.frame that will undergo a statistical test. The results of the test is saved in tests.res.t. I want to loop the varlist so that I get one results for each input in varlist
Script:
varlist <- c("Abiotrophia","Alphatorquevirus")
for (i in varlist) {
fish.test <- fish.test0[grep("i",fish.test0$group),]
column <- c("ACDC")
tests <- list()
dat_test <- sapply( column, function(colx)
lapply( unique(fish.test$Merge), function(x)
fisher.test( data.frame(
a=c(( fish.test[ which(fish.test$Merge %in% x)[2],"Present"] -
fish.test[ which(fish.test$Merge %in% x)[2], colx] ),fish.test[ which(fish.test$Merge %in% x)[2], colx]
),
b=c(( fish.test[ which(fish.test$Merge %in% x)[1],"NotPresent"] -
fish.test[ which(fish.test$Merge %in% x)[1], colx] ), fish.test[ which(fish.test$Merge %in% x)[1], colx]))) #,alternative = "greater"
) )
rownames(dat_test) <- unique(fish.test$Merge )
colnames(dat_test) <- column
tests.res <- sapply(dat_test[1:dim(dat_test)[1],1], function(x) {
c(x$estimate[1],
x$estimate[2],
ci.lower = x$conf.int[1],
ci.upper = x$conf.int[2],
p.value = x$p.value)
})
tests.res.t <- as.data.frame(t(tests.res))
}
test-data:
fish.test0 <- structure(list(Present = c(4L, 4L, 9L, 9L, 57L, 57L, 146L, 146L,
91L, 91L, 26L, 26L, 6L, 6L, 12L, 12L, 33L, 33L, 10L, 10L, 66L,
66L, 4L, 4L, 4L, 4L, 9L, 9L, 18L, 18L, 19L, 19L, 51L, 51L, 50L,
50L, 12L, 12L, 7L, 7L, 14L, 14L, 27L, 27L, 9L, 9L, 5L, 5L, 6L,
6L, 22L, 22L, 3L, 3L, 14L, 14L, 4L, 4L, 15L, 15L, 6L, 6L, 8L,
8L, 4L, 4L), NotPresent = c(11L, 11L, 44L, 44L, 126L, 126L, 532L,
532L, 382L, 382L, 97L, 97L, 14L, 14L, 43L, 43L, 85L, 85L, 41L,
41L, 336L, 336L, 19L, 19L, 27L, 27L, 67L, 67L, 108L, 108L, 81L,
81L, 240L, 240L, 258L, 258L, 47L, 47L, 31L, 31L, 82L, 82L, 110L,
110L, 63L, 63L, 178L, 178L, 672L, 672L, 451L, 451L, 120L, 120L,
104L, 104L, 47L, 47L, 387L, 387L, 94L, 94L, 300L, 300L, 133L,
133L), group = c("G__Abiotrophia_NotPresent_Anus", "G__Abiotrophia_Present_Anus",
"G__Abiotrophia_NotPresent_Bile duct", "G__Abiotrophia_Present_Bile duct",
"G__Abiotrophia_NotPresent_Bone/Soft tissue", "G__Abiotrophia_Present_Bone/Soft tissue",
"G__Abiotrophia_NotPresent_Breast", "G__Abiotrophia_Present_Breast",
"G__Abiotrophia_NotPresent_Colorectum", "G__Abiotrophia_Present_Colorectum",
"G__Abiotrophia_NotPresent_Esophagus", "G__Abiotrophia_Present_Esophagus",
"G__Abiotrophia_NotPresent_Gallbladder", "G__Abiotrophia_Present_Gallbladder",
"G__Abiotrophia_NotPresent_Head and neck", "G__Abiotrophia_Present_Head and neck",
"G__Abiotrophia_NotPresent_Kidney", "G__Abiotrophia_Present_Kidney",
"G__Abiotrophia_NotPresent_Liver", "G__Abiotrophia_Present_Liver",
"G__Abiotrophia_NotPresent_Lung", "G__Abiotrophia_Present_Lung",
"G__Abiotrophia_NotPresent_Lymphoid tissue", "G__Abiotrophia_Present_Lymphoid tissue",
"G__Abiotrophia_NotPresent_Mesothelium", "G__Abiotrophia_Present_Mesothelium",
"G__Abiotrophia_NotPresent_Nervous system", "G__Abiotrophia_Present_Nervous system",
"G__Abiotrophia_NotPresent_Ovary", "G__Abiotrophia_Present_Ovary",
"G__Abiotrophia_NotPresent_Pancreas", "G__Abiotrophia_Present_Pancreas",
"G__Abiotrophia_NotPresent_Prostate", "G__Abiotrophia_Present_Prostate",
"G__Abiotrophia_NotPresent_Skin", "G__Abiotrophia_Present_Skin",
"G__Abiotrophia_NotPresent_Small intestine", "G__Abiotrophia_Present_Small intestine",
"G__Abiotrophia_NotPresent_Stomach", "G__Abiotrophia_Present_Stomach",
"G__Abiotrophia_NotPresent_Unknown", "G__Abiotrophia_Present_Unknown",
"G__Abiotrophia_NotPresent_Urothelial tract", "G__Abiotrophia_Present_Urothelial tract",
"G__Abiotrophia_NotPresent_Uterus", "G__Abiotrophia_Present_Uterus",
"G__Alphatorquevirus_NotPresent_Bone/Soft tissue", "G__Alphatorquevirus_Present_Bone/Soft tissue",
"G__Alphatorquevirus_NotPresent_Breast", "G__Alphatorquevirus_Present_Breast",
"G__Alphatorquevirus_NotPresent_Colorectum", "G__Alphatorquevirus_Present_Colorectum",
"G__Alphatorquevirus_NotPresent_Esophagus", "G__Alphatorquevirus_Present_Esophagus",
"G__Alphatorquevirus_NotPresent_Kidney", "G__Alphatorquevirus_Present_Kidney",
"G__Alphatorquevirus_NotPresent_Liver", "G__Alphatorquevirus_Present_Liver",
"G__Alphatorquevirus_NotPresent_Lung", "G__Alphatorquevirus_Present_Lung",
"G__Alphatorquevirus_NotPresent_Pancreas", "G__Alphatorquevirus_Present_Pancreas",
"G__Alphatorquevirus_NotPresent_Skin", "G__Alphatorquevirus_Present_Skin",
"G__Alphatorquevirus_NotPresent_Urothelial tract", "G__Alphatorquevirus_Present_Urothelial tract"
), ABCD = c(3L, 2L, 17L, 6L, 34L, 18L, 240L, 53L, 321L, 73L,
87L, 25L, 6L, 3L, 20L, 8L, 15L, 7L, 19L, 4L, 265L, 42L, 6L, 1L,
4L, 2L, 22L, 4L, 70L, 13L, 54L, 12L, 116L, 33L, 58L, 11L, 6L,
2L, 26L, 6L, 42L, 8L, 74L, 18L, 19L, 3L, 52L, 0L, 288L, 5L, 377L,
17L, 110L, 2L, 19L, 3L, 21L, 2L, 298L, 9L, 60L, 6L, 68L, 1L,
89L, 3L), Total = c(15L, 15L, 53L, 53L, 183L, 183L, 678L, 678L,
473L, 473L, 123L, 123L, 20L, 20L, 55L, 55L, 118L, 118L, 51L,
51L, 402L, 402L, 23L, 23L, 31L, 31L, 76L, 76L, 126L, 126L, 100L,
100L, 291L, 291L, 308L, 308L, 59L, 59L, 38L, 38L, 96L, 96L, 137L,
137L, 72L, 72L, 183L, 183L, 678L, 678L, 473L, 473L, 123L, 123L,
118L, 118L, 51L, 51L, 402L, 402L, 100L, 100L, 308L, 308L, 137L,
137L), Merge = c("Abiotrophia_Anus", "Abiotrophia_Anus", "Abiotrophia_Bile duct",
"Abiotrophia_Bile duct", "Abiotrophia_Bone/Soft tissue", "Abiotrophia_Bone/Soft tissue",
"Abiotrophia_Breast", "Abiotrophia_Breast", "Abiotrophia_Colorectum",
"Abiotrophia_Colorectum", "Abiotrophia_Esophagus", "Abiotrophia_Esophagus",
"Abiotrophia_Gallbladder", "Abiotrophia_Gallbladder", "Abiotrophia_Head and neck",
"Abiotrophia_Head and neck", "Abiotrophia_Kidney", "Abiotrophia_Kidney",
"Abiotrophia_Liver", "Abiotrophia_Liver", "Abiotrophia_Lung",
"Abiotrophia_Lung", "Abiotrophia_Lymphoid tissue", "Abiotrophia_Lymphoid tissue",
"Abiotrophia_Mesothelium", "Abiotrophia_Mesothelium", "Abiotrophia_Nervous system",
"Abiotrophia_Nervous system", "Abiotrophia_Ovary", "Abiotrophia_Ovary",
"Abiotrophia_Pancreas", "Abiotrophia_Pancreas", "Abiotrophia_Prostate",
"Abiotrophia_Prostate", "Abiotrophia_Skin", "Abiotrophia_Skin",
"Abiotrophia_Small intestine", "Abiotrophia_Small intestine",
"Abiotrophia_Stomach", "Abiotrophia_Stomach", "Abiotrophia_Unknown",
"Abiotrophia_Unknown", "Abiotrophia_Urothelial tract", "Abiotrophia_Urothelial tract",
"Abiotrophia_Uterus", "Abiotrophia_Uterus", "Alphatorquevirus_Bone/Soft tissue",
"Alphatorquevirus_Bone/Soft tissue", "Alphatorquevirus_Breast",
"Alphatorquevirus_Breast", "Alphatorquevirus_Colorectum", "Alphatorquevirus_Colorectum",
"Alphatorquevirus_Esophagus", "Alphatorquevirus_Esophagus", "Alphatorquevirus_Kidney",
"Alphatorquevirus_Kidney", "Alphatorquevirus_Liver", "Alphatorquevirus_Liver",
"Alphatorquevirus_Lung", "Alphatorquevirus_Lung", "Alphatorquevirus_Pancreas",
"Alphatorquevirus_Pancreas", "Alphatorquevirus_Skin", "Alphatorquevirus_Skin",
"Alphatorquevirus_Urothelial tract", "Alphatorquevirus_Urothelial tract"
)), row.names = c(1L, 2L, 3L, 4L, 5L, 6L, 7L, 8L, 10L, 9L, 12L,
11L, 13L, 14L, 16L, 15L, 17L, 18L, 19L, 20L, 21L, 22L, 23L, 24L,
25L, 26L, 28L, 27L, 29L, 30L, 31L, 32L, 34L, 33L, 35L, 36L, 38L,
37L, 40L, 39L, 42L, 43L, 45L, 44L, 47L, 46L, 1011L, 1012L, 1014L,
1013L, 1015L, 1016L, 1017L, 1018L, 1019L, 1020L, 1022L, 1021L,
1023L, 1024L, 1026L, 1025L, 1027L, 1028L, 1029L, 1030L), class = "data.frame")
This is probably not an answer but it should help to improve you code. If I'm terribly wrong, I'll remove my answer right away. I have loeft out the test business which I don't understand, but your problem seems to be extraction.
The first thing is that you need to remove the quotation marks in your grep command, try:
varlist <- c("Abiotrophia","Alphatorquevirus")
for( i in varlist )
{
# extract rows which contain the variable
fish.test <- fish.test0[ grep( i, fish.test0$group ), ]
print( head( fish.test ) )
}
From what I understand, you need to define column and tests outside your loop. Does that give you more of what you want:
varlist <- c("Abiotrophia","Alphatorquevirus")
column <- "ACDC"
tests <- list()
for( i in 1 : length( varlist ) ) # index can be used later to fill the list
{
# extract rows which contain the variable
fish.test <- fish.test0[ grep( varlist[ i ], fish.test0$group ), ]
# add a column with your name of choice
fish.test <- cbind( fish.test, c( 1: length( fish.test$group ) ) )
colnames( fish.test )[ length( fish.test ) ] <- column
# write each result into your defined list
tests[[ i ]] <- fish.test
}
I am making several plots that have different x-axis limits, and I want to highlight a region of interest by adding a grey box. Even though I use the same geom_rect() command with the same alpha value in ggplot2, I get results with very different grey colors. I have looked here and here but so far have not figured out how to make these boxes the same level of transparency. Below is a reproducible example (with fake data) and the figures that it produces. Notice the different color of the grey rectangles. I want the grey to be the same across plots.
Data<-structure(list(X = c(34L, 27L, 28L, 47L, 26L, 3L, 13L, 31L, 39L,
16L, 45L, 5L, 49L, 17L, 29L, 43L, 1L, 35L, 41L, 10L, 48L, 24L,
12L, 11L, 30L, 40L, 8L, 4L, 20L, 25L, 50L, 22L, 9L, 21L, 18L,
7L, 15L, 44L, 6L, 36L, 46L, 33L, 2L, 37L, 23L, 14L, 42L, 38L,
19L, 32L, 34L, 27L, 28L, 47L, 26L, 3L, 13L, 31L, 39L, 16L, 45L,
5L, 49L, 17L, 29L, 43L, 1L, 35L, 41L, 10L, 48L, 24L, 12L, 11L,
30L, 40L, 8L, 4L, 20L, 25L, 50L, 22L, 9L, 21L, 18L, 7L, 15L,
44L, 6L, 36L, 46L, 33L, 2L, 37L, 23L, 14L, 42L, 38L, 19L, 32L
), Y = c(130L, 146L, 58L, 110L, 117L, 135L, 133L, 108L, 97L,
61L, 71L, 64L, 103L, 142L, 125L, 104L, 100L, 147L, 111L, 78L,
56L, 145L, 62L, 69L, 70L, 116L, 137L, 79L, 150L, 94L, 91L, 81L,
65L, 118L, 129L, 83L, 98L, 84L, 85L, 148L, 93L, 73L, 59L, 87L,
134L, 88L, 136L, 90L, 140L, 55L, 89L, 115L, 123L, 51L, 132L,
126L, 66L, 80L, 60L, 120L, 109L, 76L, 74L, 57L, 149L, 121L, 138L,
128L, 114L, 127L, 68L, 107L, 67L, 112L, 144L, 119L, 53L, 52L,
54L, 96L, 131L, 106L, 113L, 72L, 95L, 63L, 92L, 86L, 75L, 105L,
82L, 101L, 139L, 143L, 122L, 77L, 99L, 141L, 124L, 102L), B = structure(c(2L,
2L, 1L, 1L, 2L, 2L, 1L, 1L, 1L, 1L, 2L, 2L, 1L, 1L, 2L, 2L, 1L,
1L, 1L, 1L, 2L, 2L, 1L, 1L, 2L, 2L, 1L, 1L, 1L, 1L, 2L, 2L, 1L,
1L, 2L, 2L, 1L, 1L, 1L, 1L, 2L, 2L, 1L, 1L, 2L, 2L, 1L, 1L, 1L,
1L, 2L, 2L, 1L, 1L, 2L, 2L, 1L, 1L, 1L, 1L, 2L, 2L, 1L, 1L, 2L,
2L, 1L, 1L, 1L, 1L, 2L, 2L, 1L, 1L, 2L, 2L, 1L, 1L, 1L, 1L, 2L,
2L, 1L, 1L, 2L, 2L, 1L, 1L, 1L, 1L, 2L, 2L, 1L, 1L, 2L, 2L, 1L,
1L, 1L, 1L), class = "factor", .Label = c("no", "yes"))), .Names = c("X",
"Y", "B"), row.names = c(NA, -100L), class = "data.frame")
Data2<-structure(list(variable = c(2.49676547444708, 0.67359598601097,
0.674751772966082, 0.0317590441796792, 0.485143583939748, 1.08231639527806,
0.0732344181040914, 1.62357048819912, 0.146833215667032, 0.823157103468943,
0.240761579418538, 1.37540376416553), DOY_mid_month = c(15, 46,
75, 106, 136, 167, 197, 228, 259, 289, 320, 350)), .Names = c("variable",
"DOY_mid_month"), row.names = c(NA, -12L), class = "data.frame")
test<-ggplot(data=Data) +
geom_rect(aes(xmin=5, xmax=30, ymin=1, ymax=40), alpha = 0.02) +
geom_point(aes(x = X, y = X, colour= B), data =Data, size=2) +
theme_bw()
test2 <-ggplot(data=Data2) +
geom_rect(aes(xmin=5, xmax=30, ymin=-Inf, ymax=Inf), alpha = 0.02) +
geom_point(aes(x = DOY_mid_month, y = variable), color="black", size=4) +
scale_x_continuous("Day of Year", limits = c(0, 366)) + # Use this to add back X-axis label for the bottom plot in panel
scale_y_continuous(expression(paste("Variable", sep=""))) +
theme_bw()
Plot result from first example:
Plot result from second example:
You are currently drawing one rectangle for each row of the dataset. The more rectangles you overlap, the darker they get, which is why the longer dataset has a darker rectangle. Use annotate instead of geom_rect to draw a single rectangle.
annotate(geom = "rect", xmin=5, xmax=30, ymin=-Inf, ymax=Inf, alpha = 0.2)
If you want to stick with geom_rect you can give a one row data.frame to that layer so that each rectangle is only drawn one time. Here I use a fake dataset, although you could put your rectangle limits in the data.frame, as well.
geom_rect(data = data.frame(fake = 1),
aes(xmin = 5, xmax= 30, ymin = -Inf, ymax = Inf), alpha = 0.2)