I have temporal data of gas emissions from two species of plant, both of which have been subjected to the same treatments. With some previous help to get this code together [edit]:
soilflux = read.csv("soil_fluxes.csv")
library(ggplot2)
soilflux$Treatment <- factor(soilflux$Treatment,levels=c("L-","C","L+"))
soilplot = ggplot(soilflux, aes(factor(Week), Flux, fill=Species, alpha=Treatment)) + stat_boxplot(geom ='errorbar') + geom_boxplot()
soilplot = soilplot + labs(x = "Week", y = "Flux (mg m-2 d-1)") + theme_bw(base_size = 12, base_family = "Helvetica")
soilplot
Producing this which works well but has its flaws.
Whilst it conveys all the information I need it to, despite Google trawls and looking through here I just couldn't get the 'Treatment' part of the legend to show that L- is light and L+ darkest. I've also been told that a monochrome colour scheme is easier to differentiate hence I'm trying to get something like this where the legend is clear.
(source: biomedcentral.com)
As a workaround you could create a combined factor from species and treatment and assign the fill colors manually:
library(ggplot2)
library(RColorBrewer)
d <- expand.grid(week = factor(1:4), species = factor(c("Heisteria", "Simarouba")),
trt = factor(c("C", "L-", "L+"), levels = c("L-", "C", "L+")))
d <- d[rep(1:24, each = 30), ]
d$flux <- runif(NROW(d))
# Create a combined factor for coding the color
d$spec.trt <- interaction(d$species, d$trt, lex.order = TRUE, sep = " - ")
ggplot(d, aes(x = week, y = flux, fill = spec.trt)) +
stat_boxplot(geom ='errorbar') + geom_boxplot() +
scale_fill_manual(values = c(brewer.pal(3, "Greens"), brewer.pal(3, "Reds")))
Related
I want to plot of Gaussian distributions that are included in samp, but the size of samp may varies. Here there are 4 components but it may there are less or more components. How could I adjust the code two plot as many components as there are in samp?
ggplot(samp, aes(x=seq1, y=seq2)) +
geom_contour(mapping = aes(z=prob.1), color = "tomato") +
geom_contour(mapping = aes(z=prob.2), color = "darkblue") +
geom_contour(mapping = aes(z=prob.3), color = "green4") +
geom_contour(mapping = aes(z=prob.4), color = "purple") +
labs(x = "PC1", y = "PC2", title="Posterior probability") +
theme_bw()
The best way to do this is probably to pivot all columns whose names include the string "prob" into long format. That way, it's also easy to map the name of each probability to the color aesthetic, which gives you the option of a legend:
library(tidyverse)
ggplot(pivot_longer(samp, contains("prob")), aes(seq1, seq2, colour = name)) +
geom_contour(aes(z = value)) +
labs(x = "PC1", y = "PC2", title="Posterior probability") +
theme_bw()
Sample data used
set.seed(1)
samp <- setNames(as.data.frame(replicate(4, rnorm(100))), paste0("prob.", 1:4))
samp$seq1 <- rep(1:10, 10)
samp$seq2 <- rep(1:10, each = 10)
I need to make 5 plots of bacteria species. Each plot has a different number of species present in a range of 30-90. I want each bacteria to always have the same color in all plots, therefore I need to set an assigned color to each name.
I tried to use scale_colour_manual to create a color set but, the environment created has only 16 colors. How can I increase the number of colors present in the environment created?
the code I am using can be replicated as follow:
colour_genus <- stringi::stri_rand_strings(90, 5) #to be random names
nb.cols = nrow(colour_genus) #to set the length of my string
MyPalette = colorRampPalette(brewer.pal(12,"Set1"))(nb.cols) # the palette of choice
colGenus <- scale_color_manual(name = colour_genus, values = MyPalette)
The output formed contains only 16 values, so when I try to apply it to a figure with 90 factors, it complains I have only 16 values
abundance <- runif(90, min = 10, max = 100)
my_data <- data.frame(colour_genus, abundance)
p <- ggplot(my_data, aes(x = colour_genus, y= abundance)) +
geom_bar(aes(color = colour_genus, fill = colour_genus), stat = "identity", position = "stack") +
labs(x = "", y = "Relative Abundance\n") +
theme(panel.background = element_blank())
p + theme(legend.text= element_text(size=7, face="bold"), axis.text.x = element_text(angle = 90)) + guides(fill=guide_legend(ncol=2)) + scale_fill_manual(values=colGenus)
The following error shows:
Error: Insufficient values in manual scale. 90 needed but only 16 provided.
Thank you very much for your help.
When you know all your 90 bacci names in front of plotting, you can try.
set.seed(123)
colour_genus <- sort(stringi::stri_rand_strings(90, 5))#to be random names. I sorted the vector to illustrate the output better (optional).
MyPalette <- sample(colors(), length(colour_genus))
# named vector for scale_fill
names(MyPalette) <- colour_genus
# data
abundance <- runif(90, min = 10, max = 100)
my_data <- data.frame(colour_genus, abundance)
# two sets to show results
set1 <- my_data[20:30,]
set2 <- my_data[25:35,]
ggplot(set1, aes(x = colour_genus, y= abundance)) +
geom_col(aes(fill = colour_genus)) +
scale_fill_manual(values = MyPalette)
ggplot(set2, aes(x = colour_genus, y= abundance)) +
geom_col(aes(fill = colour_genus)) +
scale_fill_manual(values = MyPalette)
I am making a stratigraphic plot but somehow, my data points don't connect correctly.
The purpose of this plot is that the values on the x-axis are connected so you get an overview of the change in d18O throughout time (age, ma).
I've used the following script:
library(readxl)
R_pliocene_tot <- read_excel("Desktop/R_d18o.xlsx")
View(R_pliocene_tot)
install.packages("analogue")
install.packages("gridExtra")
library(tidyverse)
R_pliocene_Rtot <- R_pliocene_tot %>%
gather(key=param, value=value, -age_ma)
R_pliocene_Rtot
R_pliocene_Rtot %>%
ggplot(aes(x=value, y=age_ma)) +
geom_path() +
geom_point() +
facet_wrap(~param, scales = "free_x") +
scale_y_reverse() +
labs(x = NULL, y = "Age (ma)")
which leads to the following figure:
Something is wrong with the geom_path function, I guess, but I can't figure out what it is.
Though the comment seem solve the problem I don't think the question asked was answered. So here is some introduction about ggplot2 library regard geom_path
library(dplyr)
library(ggplot2)
# This dataset contain two group with random value for y and x run from 1->20
# The param is just to replicate the question param variable.
df <- tibble(x = rep(seq(1, 20, by = 1), 2),
y = runif(40, min = 1, max = 100),
group = c(rep("group 1", 20), rep("group 2", 20)),
param = rep("a param", 40))
df %>%
ggplot(aes(x = x, y = y)) +
# In geom_path there is group aesthetics which help the function to know
# which data point should is in which path.
# The one in the same group will be connected together.
# here I use the color to help distinct the path a bit more.
geom_path(aes(group = group, color = group)) +
geom_point() +
facet_wrap(~param, scales = "free_x") +
scale_y_reverse() +
labs(x = NULL, y = "Age (ma)")
In your data which work well with group = 1 I guessed all data points belong to one group and you just want to draw a line connect all those data point. So take my data example above and draw with aesthetics group = 1, you can see the result that have two line similar to the above example but now the end point of group 1 is now connected with the starting point of group 2.
So all data point is now on one path but the order of how they draw is depend on the order they appear in the data. (I keep the color just to help see it a bit clearer)
df %>%
ggplot(aes(x = x, y = y)) +
geom_path(aes(group = 1, color = group)) +
geom_point() +
facet_wrap(~param, scales = "free_x") +
scale_y_reverse() +
labs(x = NULL, y = "Age (ma)")
Hope this give you better understanding of ggplot2::geom_path
I am using the fivethirtyeight bechdel dataset, located here https://github.com/rudeboybert/fivethirtyeight, and am attempting to recreate the first plot shown in the article here https://fivethirtyeight.com/features/the-dollar-and-cents-case-against-hollywoods-exclusion-of-women/. I am having trouble getting the years to group together similarly to how they did in the article.
This is the current code I have:
ggplot(data = bechdel, aes(year)) +
geom_histogram(aes(fill = clean_test), binwidth = 5, position = "fill") +
scale_fill_manual(breaks = c("ok", "dubious", "men", "notalk", "nowomen"),
values=c("red", "salmon", "lightpink", "dodgerblue",
"blue")) +
theme_fivethirtyeight()
I see where you were going with using the histogram geom but this really looks more like a categorical bar chart. Once you take that approach it's easier, after a bit of ugly code to get the correct labels on the year columns.
The bars are stacked in the wrong order on this one, and there needs to be some formatting applied to look like the 538 chart, but I'll leave that for you.
library(fivethirtyeight)
library(tidyverse)
library(ggthemes)
library(scales)
# Create date range column
bechdel_summary <- bechdel %>%
mutate(date.range = ((year %/% 10)* 10) + ((year %% 10) %/% 5 * 5)) %>%
mutate(date.range = paste0(date.range," - '",substr(date.range + 5,3,5)))
ggplot(data = bechdel_summary, aes(x = date.range, fill = clean_test)) +
geom_bar(position = "fill", width = 0.95) +
scale_y_continuous(labels = percent) +
theme_fivethirtyeight()
ggplot
I have a data.frame that looks something like this:
HSP90AA1 SSH2 ACTB TotalTranscripts
ESC_11_TTCGCCAAATCC 8.053308 12.038484 10.557234 33367.23
ESC_10_TTGAGCTGCACT 9.430003 10.687959 10.437068 30285.41
ESC_11_GCCGCGTTATAA 7.953726 9.918988 10.078192 30133.94
ESC_11_GCATTCTGGCTC 11.184402 11.056144 8.316846 24857.07
ESC_11_GTTACATTTCAC 11.943733 11.004500 9.240883 23629.00
ESC_11_CCGTTGCCCCTC 7.441695 9.774733 7.566619 22792.18
The TotalTranscripts column is sorted in descending order. What I'd like to do is generate three bar graphs using ggplot2 with each bar graph corresponding to each column of the data.frame with the exception of TotalTranscripts. I'd like the bar graphs to be ordered by TotalTranscripts just as the data.frame. I would be ideal to have these bar graphs on one plot using a facet wrap.
Any help would be greatly appreciated! Thank you!
EDIT: Here is my current code using barplot().
cells = "ESC"
genes = c("HSP90AA1", "SSH2", "ACTB")
g = data[genes,grep(cells, colnames(data))]
g = data.frame(t(g), colSums(data)[grep(cells, colnames(data))])
colnames(g)[ncol(g)] = "TotalTranscripts"
g = g[order(g$TotalTranscripts, decreasing=T), , drop=F]
barplot(as.matrix(g[1]), beside=TRUE, names.arg=paste(rownames(g)," (",g$TotalTranscripts,")",sep=""), las=2, col="light blue", cex.names=0.3, main=paste(colnames(g)[1], "\nCells sorted by total number of transcripts (colSums)", sep=""))
This will generate a plot that looks like this.
Again, the problem I seem to be having here is how to have multiple of these plots on the same image. I would like to add 20+ columns to this data.frame but I've cut this down to 3 for the sake of simplicity.
EDIT: Current code incorporating the answer below
cells = "ESC"
genes = rownames(data[x,])[1:8]
# genes = c("HSP90AA1", "SSH2", "ACTB")
g = data[genes,grep(cells, colnames(data))]
g = data.frame(t(g), colSums(data)[grep(cells, colnames(data))])
colnames(g)[ncol(g)] = "TotalTranscripts"
g = g[order(g$TotalTranscripts, decreasing=T), , drop=F]
g$rowz <- row.names(g)
g$Cells <- reorder(g$rowz, rev(g$TotalTranscripts))
df1 <- melt(g, id.vars = c("Cells", "TotalTranscripts"), measure.vars=genes)
ggplot(df1, aes(x = Cells, y = value)) + geom_bar(stat = "identity") +
theme(axis.title.x=element_blank(), axis.text.x = element_blank()) +
facet_wrap(~ variable, scales = "free") +
theme_bw() + theme(axis.text.x = element_text(angle = 90))
Here is the example data for anybody else:
df <- structure(list(HSP90AA1 = c(8.053308, 9.430003, 7.953726, 11.184402,
11.943733, 7.441695), SSH2 = c(12.038484, 10.687959, 9.918988,
11.056144, 11.0045, 9.774733), ACTB = c(10.557234, 10.437068,
10.078192, 8.316846, 9.240883, 7.566619), TotalTranscripts = c(33367.23,
30285.41, 30133.94, 24857.07, 23629, 22792.18)), .Names = c("HSP90AA1",
"SSH2", "ACTB", "TotalTranscripts"), class = "data.frame", row.names = c("ESC_11_TTCGCCAAATCC",
"ESC_10_TTGAGCTGCACT", "ESC_11_GCCGCGTTATAA", "ESC_11_GCATTCTGGCTC",
"ESC_11_GTTACATTTCAC", "ESC_11_CCGTTGCCCCTC"))
And here is a solution:
#New column for row names so they can be used as x-axis elements
df$rowz <- row.names(df)
#Explicitly order the rows (see the Kohske link)
df$rowz1 <- reorder(df$rowz, rev(df$TotalTranscripts))
library(reshape2)
#Melt the data from wide to long
df1 <- melt(df, id.vars = c("rowz1", "TotalTranscripts"),
measure.vars = c("HSP90AA1", "SSH2", "ACTB"))
library(ggplot2)
gp <- ggplot(df1, aes(x = rowz1, y = value)) + geom_bar(stat = "identity") +
facet_wrap(~ variable, scales = "free") +
theme_bw()
gp + theme(axis.text.x = element_text(angle = 90))
This example by Kohske is a constant reference for me on ordering elements in ggplot2.
If you have many columns, but the same six ESC complexes, you can switch the groupings, i.e. x = variable and facet_wrap(~ rowz1), but this fundamentally changes how you are visualizing/comparing your data. Also, consider facet_grid(row ~ column) if you can organize the columns by 2 components (Columns being the data that are melted into 'variable' and 'value').
And this additional SO solution isn't related to your question, but it is an elegant way to reorder elements in each facet by their values (for future reference).
Finally, the method that will give you the finest control is to plot each graph separately and combine the grobs. Baptiste's packages like gridExtra and gtable are useful for these tasks.
**EDIT in response to new information from OP**
The OP has subsequently asked how to visualize the data, especially when there are more ESC categorical variables (up to 600+).
Here are some examples, with the big caveat that with many categorical variables, they should be grouped or converted to a continuous variable somehow.
#Plot colour to a few discrete, categorical variables
gp + aes(fill = rowz1) +
theme(axis.text.x = element_blank(), axis.ticks.x = element_blank()) +
labs(x = NULL, fill = "Cell", title = "Discrete categorical variables")
#Plot colour on a continuous scale.
#Ultimately, not appropriate for this example! (but shown for reference)
#More appropriate: fill = TotalTranscripts
gp + aes(fill = as.numeric(rowz1)) +
theme(axis.text.x = element_blank(), axis.ticks.x = element_blank()) +
labs(x = NULL, title = "Continuous variables (legend won't work for many values)") +
scale_fill_gradient2(name = "Cell",
breaks = as.numeric(df1$rowz1),
labels = df1$rowz1,
midpoint=median(as.numeric(df1$rowz1)))
#x is continuous, colour plotted to the categorical variable.
#Same caveats as earlier.
gp1 <- ggplot(df1, aes(x = TotalTranscripts/1000, y = value, colour = rowz1)) +
geom_point(size=3) + facet_wrap(~ variable, scales = "free") +
labs(title = "X is an actual continuous variable") +
theme_bw() + labs(x = bquote("Total Transcripts,"~10^3), colour = "Cell")
gp1