Having issues using the lme4 predict function on my mixed models - r

Question

I’m having a bit of a struggle trying to use the lme4 predict function on my mixed models. When making predications I want to be able to set some of my explanatory variables to a specified level but average across others.
Here’s some made up data that is a simplified, nonsense version of my original dataset:
a <- data.frame(
TLR4=factor(rep(1:3, each=4, times=4)),
repro.state=factor(rep(c("a","j"),each=6,times=8)),
month=factor(rep(1:2,each=8,times=6)),
sex=factor(rep(1:2, each=4, times=12)),
year=factor(rep(1:3, each =32)),
mwalkeri=(sample(0:15, 96, replace=TRUE)),
AvM=(seq(1:96))
)
The AvM number is the water vole identification number. The response variable (mwalkeri) is a count of the number of fleas on each vole. The main explanatory variable I am interested in is Tlr4 which is a gene with 3 different genotypes (coded 1, 2 and 3). The other explanatory variables included are reproductive state (adult or juvenile), month (1 or 2), sex (1 or 2) and year (1, 2 or 3). My model looks like this (of course this model is now inappropriate for the made up data but that shouldn't matter):
install.packages("lme4")
library(lme4)
mm <- glmer(mwalkeri~TLR4+repro.state+month+sex+year+(1|AvM), data=a,
family=poisson,control=glmerControl(optimizer="bobyqa"))`
summary(mm)
I want to make predictions about parasite burden for each different Tlr4 genotype while accounting for all the other covariates. To do this I created a new dataset to specify the level I wanted to set each of the explanatory variables to and used the predict function:
b <- data.frame(
TLR4=factor(1:3),
repro.state=factor(c("a","a","a")),
month=factor(rep(1, times=3)),
sex=factor(rep(1, times=3)),
year=factor(rep(1, times=3))
)
predict(mm, newdata=b, re.form=NA, type="response")
This did work but I would really prefer to average across years instead of setting year to one particular level. However, whenever I attempt to average year I get this error message:
Error in model.frame.default(delete.response(Terms), newdata, na.action = na.action, : factor year has new level
Is it possible for me to average across years instead of selecting a specified level? Also, I've not worked out how to get the standard error associated with these predictions. The only way I've been able to get standard error for predictions was using the lsmeans() function (from the lsmeans package):
c <- lsmeans(mm, "TLR4", type="response")
summary(c, type="response")
Which automatically generates the standard error. However, this is generated by averaging across all the other explanatory variables. I'm sure it’s probably possible to change that but I would rather use the predict() function if I can. My goal is to create a graph with Tlr4 genotype on the x-axis and predicted parasite burden on the y-axis to demonstrate the predicted differences in parasite burden for each genotype while all other significant covariants are accounted for.

Answer 1

You might be interested in the merTools package which includes a couple of functions for creating datasets of counterfactuals and then making predictions on that new data to explore the substantive impact of variables on the outcome. A good example of this comes from the README and the package vignette:
Let's take the case where we want to explore the impact of a model with an interaction term between a category and a continuous predictor. First, we fit a model with interactions:
data(VerbAgg)
fmVA <- glmer(r2 ~ (Anger + Gender + btype + situ)^2 +
(1|id) + (1|item), family = binomial,
data = VerbAgg)
Now we prep the data using the draw function in merTools. Here we draw the average observation from the model frame. We then wiggle the data by expanding the dataframe to include the same observation repeated but with different values of the variable specified by the var parameter. Here, we expand the dataset to all values of btype, situ, and Anger.
# Select the average case
newData <- draw(fmVA, type = "average")
newData <- wiggle(newData, var = "btype", values = unique(VerbAgg$btype))
newData <- wiggle(newData, var = "situ", values = unique(VerbAgg$situ))
newData <- wiggle(newData, var = "Anger", values = unique(VerbAgg$Anger))
head(newData, 10)
#> r2 Anger Gender btype situ id item
#> 1 N 20 F curse other 5 S3WantCurse
#> 2 N 20 F scold other 5 S3WantCurse
#> 3 N 20 F shout other 5 S3WantCurse
#> 4 N 20 F curse self 5 S3WantCurse
#> 5 N 20 F scold self 5 S3WantCurse
#> 6 N 20 F shout self 5 S3WantCurse
#> 7 N 11 F curse other 5 S3WantCurse
#> 8 N 11 F scold other 5 S3WantCurse
#> 9 N 11 F shout other 5 S3WantCurse
#> 10 N 11 F curse self 5 S3WantCurse
Now we simply pass this new dataset to predictInterval in order to generate predictions for these counterfactuals. Then we plot the predicted values against the continuous variable, Anger, and facet and group on the two categorical variables situ and btype respectively.
plotdf <- predictInterval(fmVA, newdata = newData, type = "probability",
stat = "median", n.sims = 1000)
plotdf <- cbind(plotdf, newData)
ggplot(plotdf, aes(y = fit, x = Anger, color = btype, group = btype)) +
geom_point() + geom_smooth(aes(color = btype), method = "lm") +
facet_wrap(~situ) + theme_bw() +
labs(y = "Predicted Probability")