Interpreting mcmc output using glmmadmb - r

I'm trying to evaluate the output from a negative binomial mixed model using glmmadmb. To summarize the output I'm comparing the summary function with output forom the mcmc option. I have run this model:
pre1 <- glmmadmb(walleye~(1|year.center) + (1|Site) ,data=pre,
family="nbinom2",link="log",
mcmc=TRUE,mcmc.opts=mcmcControl(mcmc=1000))
I have two random intercepts: year and site. Year has 33 levels and site has 15.
The random effect parameter estimate for site and year from summary(pre1) do not seem to agree with the posterior distribution from the mcmc output. I am using the 50% confidence interval as the estimate that should coincide with the parameter estimate from the summary function. Is that incorrect? Is there a way to obtain an error around the random effect parameter using the summary function to gauge whether this is variance issue? I tried using postvar=T with ranef but that did not work. Also, Is there a way to format the mcmc output with informative row names to ensure I'm using the proper estimates?
summary output from glmmabmb:
summary(pre1)
Call:
glmmadmb(formula = walleye ~ (1 | year.center) + (1 | Site),
data = pre, family = "nbinom2", link = "log", mcmc = TRUE,
mcmc.opts = mcmcControl(mcmc = 1000))
AIC: 4199.8
Coefficients:
Estimate Std. Error z value Pr(>|z|)
(Intercept) 3.226 0.154 21 <2e-16 ***
Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
Number of observations: total=495, year.center=33, Site=15
Random effect variance(s):
Group=year.center
Variance StdDev
(Intercept) 0.1085 0.3295
Group=Site
Variance StdDev
(Intercept) 0.2891 0.5377
Negative binomial dispersion parameter: 2.0553 (std. err.: 0.14419)
Log-likelihood: -2095.88
mcmc output:
m <- as.mcmc(pre1$mcmc)
CI <- t(apply(m,2,quantile,c(0.025,0.5,0.975)))
2.5% 50% 97.5%
(Intercept) 2.911667943 3.211775843 3.5537371345
tmpL.1 0.226614903 0.342206509 0.4600328729
tmpL.2 0.395353518 0.554211483 0.8619127547
alpha 1.789687691 2.050871824 2.3175742167
u.01 0.676758365 0.896844797 1.0726750539
u.02 0.424938481 0.588191585 0.7364795440
these estimates continue to u.48 to include year and site specific coefficients.
Thank you in advance for any thoughts on this issue.
Tiffany

The random effect parameter estimate for site and year from summary(pre1) do not seem to agree with the posterior distribution from the mcmc output. I am using the 50% confidence interval as the estimate that should coincide with the parameter estimate from the summary function. Is that incorrect?
It's not the 50% confidence interval, it's the 50% quantile (i.e. the median). The point estimates from the Laplace approximation of the among-year and among-site standard deviations respectively are {0.3295,0.5377}, which seem quite close to the MCMC median estimates {0.342206509,0.554211483} ... as discussed below, the MCMC tmpL parameters are the random-effects standard deviations, not the variances -- this might be the main cause of your confusion?
Is there a way to obtain an error around the random effect parameter using the summary function to gauge whether this is variance issue? I tried using postvar=T with ranef but that did not work.
The lme4 package (not the glmmadmb package) allows estimates of the variances of the conditional modes (i.e. the random effects associated with particular levels) via ranef(...,condVar=TRUE) (postVar=TRUE is now deprecated). The equivalent information on the uncertainty of the conditional modes is available via ranef(model,sd=TRUE) (see ?ranef.glmmadmb).
However, I think you might be looking for the $S (variance-covariance matrices) and $sd_S (Wald standard errors of the variance-covariance estimates) instead (although as stated above, I don't think there's really a problem).
Also, Is there a way to format the mcmc output with informative row names to ensure I'm using the proper estimates?
See p. 15 of vignette("glmmADMB",package="glmmADMB"):
The MCMC output in glmmADMB is not completely translated. It includes, in order:
pz zero-inflation parameter (raw)
fixed effect parameters Named in the same way as the results of coef() or
fixef().
tmpL variances (standard-deviation scale)
tmpL1 correlation/off-diagonal elements of variance-covariance matrices (off-diagonal elements of the Cholesky factor of the correlation matrix'). (If you need to transform these to correlations, you will need to construct the relevant matrices with 1 on the diagonal and compute the cross-product, CC^T (see tcrossprod); if this makes no sense to you, contact the maintainers)
alpha overdispersion/scale parameter
u random effects (unscaled: these can be scaled using the estimated random-effects standard deviations from VarCorr())

Related

How to implement a non linear model regression in R

I am quite new to both R and Statistics and really need your help. I should analyze some data to find an analytical model that describes it.
I have 2 response (y1,y2) and (4 predictors).
I thought of performing the analysis using R and followed these steps:
1) For each response, I tested a linear model (lm command) and I found:
Call:
lm(formula = data_mass$m ~ ., data = data_mass)
Residuals:
Min 1Q Median 3Q Max
-7.805e-06 -1.849e-06 -1.810e-07 2.453e-06 7.327e-06
Coefficients:
Estimate Std. Error t value Pr(>|t|)
(Intercept) -1.367e-04 1.845e-05 -7.413 1.47e-06 ***
d 1.632e-04 1.134e-05 14.394 1.42e-10 ***
L 2.630e-08 1.276e-07 0.206 0.83927
D 1.584e-05 5.103e-06 3.104 0.00682 **
p 1.101e-06 1.195e-07 9.215 8.46e-08 ***
---
Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
Residual standard error: 4.472e-06 on 16 degrees of freedom
Multiple R-squared: 0.9543, Adjusted R-squared: 0.9429
F-statistic: 83.51 on 4 and 16 DF, p-value: 1.645e-10
2) So I analyzed how good the model is by taking a look at plot(model) graphs.
Looking at the "residual vs fitted value" plot, the model should not be linear!! Is it correct?
3) I tried to eliminate some factors (like "L") and to introduce some quadratic terms (d^2 ; D^2), but the "residual vs fitted value" plot has the same trend.
What can I do now? Should I use a non-linear model?
Thank you to everyone can help me =)
UPDATE:
Thank you again. I attached graph of plot(model) and DATA. The responses are m, Fz and the predictors d,L,D,p. The model is a linear model of response m.
[Residual vs Fitted][1]
[Normal Q-Q][2]
[Scale Location][3]
[Residual vs Leverage][4]
[DATA][5]
enter code here
Looking the "residual vs fitted value" plot the model should not be linear!! Is it correct?
Yes and no. If absolute value of the residuals have strong correlation with the fitted values, that could mean heteroscedasticity (heterogeneity of variance).
Then the residuals would not be equally spread along the fitted values. And heteroscedasticity is one of the thing you could look at on fitted vs residual graph, because it can invalidate statistical tests such as *t*-test or lm. You could also confirm it with scale-location plot (which is quite similar to this but slightly better).
On the other hand nonlinear distribution indicate nonlinearity and would probably want to change the structure of your model. Though you don´t wont neither linear, nor nonlinear relationship between residuals and fitted values: in ideal case scenario values should be more or less randomly and symmetrically scattered around 0 between two parallel lines with 0 slope.
You can find more discussion on the issue here: 1 2 3
What can I do now? Should I use a non-linear model?
If your diagnostic plots indicate nonlinearity, you may want to change/restructure/readjust your model (or transform the data) - there is some discussion on the options here

Back-transforming gamma GLM to natural units to be able to predict values in unsampled locations

I'm working with ecological data, where I have used cameras to sample animal detections (converted to biomass) and run various models to identify the best fitting model, chosen through looking at diagnostic plots, AIC and parameter effect size. The model is a gamma GLM (due to biomass having a continuous response), with a log link. The chosen model has the predictor variables of distance to water ("dist_water") and distance to forest patch ("dist_F3"). This is the model summary:
glm(formula = RAI_biomass ~ Dist_water.std + Dist_F3.std, family = Gamma(link = "log"),
data = biomass_RAI)
Deviance Residuals:
Min 1Q Median 3Q Max
-2.3835 -1.0611 -0.3937 0.4355 1.5923
Coefficients:
Estimate Std. Error t value Pr(>|t|)
(Intercept) 5.3577 0.2049 26.143 2.33e-16 ***
Dist_water.std -0.7531 0.2168 -3.474 0.00254 **
Dist_F3.std 0.5831 0.2168 2.689 0.01452 *
---
Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
(Dispersion parameter for Gamma family taken to be 0.9239696)
Null deviance: 41.231 on 21 degrees of freedom
Residual deviance: 24.232 on 19 degrees of freedom
AIC: 287.98
Number of Fisher Scoring iterations: 7
The covariates were standardised prior to running the model. What I need to do now is to back-transform this model into natural units in order to predict biomass values at unsampled locations (in this case, farms). I made a table of each farm and their respective distance to water, and distance to forest patch. I thought the way to do this would be to use the exp(coef(biomass_glm)), but when I did this the dist_water.std coefficient changed direction and became positive.
exp(coef(biomass_glm8))
## Intercept Dist_water.std Dist_F3.std
## 212.2369519 0.4709015 1.7915026
To me this seems problematic, as in the original GLM, an increasing distance to water meant a decrease in biomass (this makes sense) - but now we are seeing the opposite? The calculated biomass response had a very narrow range, from 210.97-218.9331 (for comparison, in the original data, biomass ranged from 3.04-2227.99.
I then tried to take the exponent of the entire model, without taking the exponent of each coefficient individually:
farms$biomass_est2 <- exp(5.3577 + (-0.7531*farms$Farm_dist_water_std) + (0.5831*farms$Farm_dist_F3_std))
and this gave me a new biomass response that makes a bit more sense, i.e. more variation given the variation in the two covariates (2.93-1088.84).
I then tried converting the coefficient estimates by doing e^B - 1, which gave again different results, although most similar to the ones obtained through exp(coef(biomass_glm)):
(e^(-0.7531))-1 #dist_water = -0.5290955
(e^(0.5831))-1 #dist_F3 = 0.7915837
(e^(5.3577))-1 #intercept = 211.2362
My question is, why are these estimates different, and what is the best way to take this gamma GLM with a log link and convert it into a format that can be used to calculate predicted values? Any help would be greatly appreciated!

R: Calculate and interpret odds ratio in logistic regression

I am having trouble interpreting the results of a logistic regression. My outcome variable is Decision and is binary (0 or 1, not take or take a product, respectively).
My predictor variable is Thoughts and is continuous, can be positive or negative, and is rounded up to the 2nd decimal point.
I want to know how the probability of taking the product changes as Thoughts changes.
The logistic regression equation is:
glm(Decision ~ Thoughts, family = binomial, data = data)
According to this model, Thoughts has a significant impact on probability of Decision (b = .72, p = .02). To determine the odds ratio of Decision as a function of Thoughts:
exp(coef(results))
Odds ratio = 2.07.
Questions:
How do I interpret the odds ratio?
Does an odds ratio of 2.07 imply that a .01 increase (or decrease) in Thoughts affect the odds of taking (or not taking) the product by 0.07 OR
Does it imply that as Thoughts increases (decreases) by .01, the odds of taking (not taking) the product increase (decrease) by approximately 2 units?
How do I convert odds ratio of Thoughts to an estimated probability of Decision?
Or can I only estimate the probability of Decision at a certain Thoughts score (i.e. calculate the estimated probability of taking the product when Thoughts == 1)?
The coefficient returned by a logistic regression in r is a logit, or the log of the odds. To convert logits to odds ratio, you can exponentiate it, as you've done above. To convert logits to probabilities, you can use the function exp(logit)/(1+exp(logit)). However, there are some things to note about this procedure.
First, I'll use some reproducible data to illustrate
library('MASS')
data("menarche")
m<-glm(cbind(Menarche, Total-Menarche) ~ Age, family=binomial, data=menarche)
summary(m)
This returns:
Call:
glm(formula = cbind(Menarche, Total - Menarche) ~ Age, family = binomial,
data = menarche)
Deviance Residuals:
Min 1Q Median 3Q Max
-2.0363 -0.9953 -0.4900 0.7780 1.3675
Coefficients:
Estimate Std. Error z value Pr(>|z|)
(Intercept) -21.22639 0.77068 -27.54 <2e-16 ***
Age 1.63197 0.05895 27.68 <2e-16 ***
---
Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
(Dispersion parameter for binomial family taken to be 1)
Null deviance: 3693.884 on 24 degrees of freedom
Residual deviance: 26.703 on 23 degrees of freedom
AIC: 114.76
Number of Fisher Scoring iterations: 4
The coefficients displayed are for logits, just as in your example. If we plot these data and this model, we see the sigmoidal function that is characteristic of a logistic model fit to binomial data
#predict gives the predicted value in terms of logits
plot.dat <- data.frame(prob = menarche$Menarche/menarche$Total,
age = menarche$Age,
fit = predict(m, menarche))
#convert those logit values to probabilities
plot.dat$fit_prob <- exp(plot.dat$fit)/(1+exp(plot.dat$fit))
library(ggplot2)
ggplot(plot.dat, aes(x=age, y=prob)) +
geom_point() +
geom_line(aes(x=age, y=fit_prob))
Note that the change in probabilities is not constant - the curve rises slowly at first, then more quickly in the middle, then levels out at the end. The difference in probabilities between 10 and 12 is far less than the difference in probabilities between 12 and 14. This means that it's impossible to summarise the relationship of age and probabilities with one number without transforming probabilities.
To answer your specific questions:
How do you interpret odds ratios?
The odds ratio for the value of the intercept is the odds of a "success" (in your data, this is the odds of taking the product) when x = 0 (i.e. zero thoughts). The odds ratio for your coefficient is the increase in odds above this value of the intercept when you add one whole x value (i.e. x=1; one thought). Using the menarche data:
exp(coef(m))
(Intercept) Age
6.046358e-10 5.113931e+00
We could interpret this as the odds of menarche occurring at age = 0 is .00000000006. Or, basically impossible. Exponentiating the age coefficient tells us the expected increase in the odds of menarche for each unit of age. In this case, it's just over a quintupling. An odds ratio of 1 indicates no change, whereas an odds ratio of 2 indicates a doubling, etc.
Your odds ratio of 2.07 implies that a 1 unit increase in 'Thoughts' increases the odds of taking the product by a factor of 2.07.
How do you convert odds ratios of thoughts to an estimated probability of decision?
You need to do this for selected values of thoughts, because, as you can see in the plot above, the change is not constant across the range of x values. If you want the probability of some value for thoughts, get the answer as follows:
exp(intercept + coef*THOUGHT_Value)/(1+(exp(intercept+coef*THOUGHT_Value))
Odds and probability are two different measures, both addressing the same aim of measuring the likeliness of an event to occur. They should not be compared to each other, only among themselves!
While odds of two predictor values (while holding others constant) are compared using "odds ratio" (odds1 / odds2), the same procedure for probability is called "risk ratio" (probability1 / probability2).
In general, odds are preferred against probability when it comes to ratios since probability is limited between 0 and 1 while odds are defined from -inf to +inf.
To easily calculate odds ratios including their confident intervals, see the oddsratio package:
library(oddsratio)
fit_glm <- glm(admit ~ gre + gpa + rank, data = data_glm, family = "binomial")
# Calculate OR for specific increment step of continuous variable
or_glm(data = data_glm, model = fit_glm,
incr = list(gre = 380, gpa = 5))
predictor oddsratio CI.low (2.5 %) CI.high (97.5 %) increment
1 gre 2.364 1.054 5.396 380
2 gpa 55.712 2.229 1511.282 5
3 rank2 0.509 0.272 0.945 Indicator variable
4 rank3 0.262 0.132 0.512 Indicator variable
5 rank4 0.212 0.091 0.471 Indicator variable
Here you can simply specify the increment of your continuous variables and see the resulting odds ratios. In this example, the response admit is 55 times more likely to occur when predictor gpa is increased by 5.
If you want to predict probabilities with your model, simply use type = response when predicting your model. This will automatically convert log odds to probability. You can then calculate risk ratios from the calculated probabilities. See ?predict.glm for more details.
I found this epiDisplay package, works fine! It might be useful for others but note that your confidence intervals or exact results will vary according to the package used so it is good to read the package details and chose the one that works well for your data.
Here is a sample code:
library(epiDisplay)
data(Wells, package="carData")
glm1 <- glm(switch~arsenic+distance+education+association,
family=binomial, data=Wells)
logistic.display(glm1)
Source website
The above formula to logits to probabilities, exp(logit)/(1+exp(logit)), may not have any meaning. This formula is normally used to convert odds to probabilities. However, in logistic regression an odds ratio is more like a ratio between two odds values (which happen to already be ratios). How would probability be defined using the above formula? Instead, it may be more correct to minus 1 from the odds ratio to find a percent value and then interpret the percentage as the odds of the outcome increase/decrease by x percent given the predictor.

Confidence Interval Based on Asymptotic Normality in lmer model

Why is not confint.default which is based on asymptotic normality doesn't work for lmer model ?
fit <- lmer(y~(1|operator)+(1|part),data=dat)
Linear mixed model fit by REML ['lmerMod']
Formula: y ~ (1 | operator) + (1 | part)
Data: dat
REML criterion at convergence: 409.3913
Random effects:
Groups Name Std.Dev.
part (Intercept) 3.2018
operator (Intercept) 0.1031
Residual 0.9398
Number of obs: 120, groups: part, 20; operator, 3
Fixed Effects:
(Intercept)
22.39
confint.default(fit)
Error in as.integer(x) :
cannot coerce type 'S4' to vector of type 'integer'
What is the error saying ? How can I get confidence interval based on asymptotic normality for lmer model ?
Don't use confint.default(), just use confint(). The methods to calculate confidence intervals are different for the different model types. You can see the different methods with methods(confint). The "correct" version of the function is called based on the class of the first object you pass to the function. Directly calling one of the methods usually isn't a good idea.
There are options for how to calculate the bounds for the lmer objects. Look at the help page for ?confint.merMod to see the options unique to that model type.
#MrFlick is correct, but it may be worth adding that confint.merMod() gives likelihood profile CIs by default; confint(.,method="Wald") will give the confidence intervals based on asymptotic normality:
‘"Wald"’: approximating the confidence intervals (of fixed-effect
parameters only; all variance-covariance parameters CIs will
be returned as ‘NA’) based on the estimated local curvature
of the likelihood surface;
(this is obvious from the help page, but is probably worth restating here).

What is the difference between Multiple R-squared and Adjusted R-squared in a single-variate least squares regression?

Could someone explain to the statistically naive what the difference between Multiple R-squared and Adjusted R-squared is? I am doing a single-variate regression analysis as follows:
v.lm <- lm(epm ~ n_days, data=v)
print(summary(v.lm))
Results:
Call:
lm(formula = epm ~ n_days, data = v)
Residuals:
Min 1Q Median 3Q Max
-693.59 -325.79 53.34 302.46 964.95
Coefficients:
Estimate Std. Error t value Pr(>|t|)
(Intercept) 2550.39 92.15 27.677 <2e-16 ***
n_days -13.12 5.39 -2.433 0.0216 *
---
Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
Residual standard error: 410.1 on 28 degrees of freedom
Multiple R-squared: 0.1746, Adjusted R-squared: 0.1451
F-statistic: 5.921 on 1 and 28 DF, p-value: 0.0216
The "adjustment" in adjusted R-squared is related to the number of variables and the number of observations.
If you keep adding variables (predictors) to your model, R-squared will improve - that is, the predictors will appear to explain the variance - but some of that improvement may be due to chance alone. So adjusted R-squared tries to correct for this, by taking into account the ratio (N-1)/(N-k-1) where N = number of observations and k = number of variables (predictors).
It's probably not a concern in your case, since you have a single variate.
Some references:
How high, R-squared?
Goodness of fit statistics
Multiple regression
Re: What is "Adjusted R^2" in Multiple Regression
The Adjusted R-squared is close to, but different from, the value of R2. Instead of being based on the explained sum of squares SSR and the total sum of squares SSY, it is based on the overall variance (a quantity we do not typically calculate), s2T = SSY/(n - 1) and the error variance MSE (from the ANOVA table) and is worked out like this: adjusted R-squared = (s2T - MSE) / s2T.
This approach provides a better basis for judging the improvement in a fit due to adding an explanatory variable, but it does not have the simple summarizing interpretation that R2 has.
If I haven't made a mistake, you should verify the values of adjusted R-squared and R-squared as follows:
s2T <- sum(anova(v.lm)[[2]]) / sum(anova(v.lm)[[1]])
MSE <- anova(v.lm)[[3]][2]
adj.R2 <- (s2T - MSE) / s2T
On the other side, R2 is: SSR/SSY, where SSR = SSY - SSE
attach(v)
SSE <- deviance(v.lm) # or SSE <- sum((epm - predict(v.lm,list(n_days)))^2)
SSY <- deviance(lm(epm ~ 1)) # or SSY <- sum((epm-mean(epm))^2)
SSR <- (SSY - SSE) # or SSR <- sum((predict(v.lm,list(n_days)) - mean(epm))^2)
R2 <- SSR / SSY
The R-squared is not dependent on the number of variables in the model. The adjusted R-squared is.
The adjusted R-squared adds a penalty for adding variables to the model that are uncorrelated with the variable your trying to explain. You can use it to test if a variable is relevant to the thing your trying to explain.
Adjusted R-squared is R-squared with some divisions added to make it dependent on the number of variables in the model.
Note that, in addition to number of predictive variables, the Adjusted R-squared formula above also adjusts for sample size. A small sample will give a deceptively large R-squared.
Ping Yin & Xitao Fan, J. of Experimental Education 69(2): 203-224, "Estimating R-squared shrinkage in multiple regression", compares different methods for adjusting r-squared and concludes that the commonly-used ones quoted above are not good. They recommend the Olkin & Pratt formula.
However, I've seen some indication that population size has a much larger effect than any of these formulas indicate. I am not convinced that any of these formulas are good enough to allow you to compare regressions done with very different sample sizes (e.g., 2,000 vs. 200,000 samples; the standard formulas would make almost no sample-size-based adjustment). I would do some cross-validation to check the r-squared on each sample.

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