How to use the "how" function for an unbalanced repeated design - r

I have a set of control and treated plots which had been sampled during years. I run the prc function in the vegan package and want to perform a permutation test to check whether control vs treated plots significantly differ during years. As my data is unbalanced, I can not use strata function. my code look like:
library(vegan)
year=as.factor(c(rep(1995,8),rep(1999,8),rep(2001,8),rep(2013,4),rep(1995,4),
rep(1999,4),rep(2001,4),rep(2013,4)))
treatment=as.factor(c(rep("control",28),rep("treated",16)))
I've written this, but I'm sure that it is wrong because the treatment is missing here:
h1 <- how(within = Within(type = "series", mirror = F),
blocks = year, nperm = 999
)
Any suggestions is greatly appreciated.

Under the null hypothesis, samples from the control or treated groups are exchangeable and hence you don't want them in the permutation design; you really want to permute them to generate the permutation-based null distribution for the test statistic.
The permutation design is there to indicate what isn't exchangeable.
You haven't explained why you want samples within the blocks to be permuted in series; why are samples within years also time series? If they're not, you don't want this.
You only need to worry about imbalance if you want to permute the strata. Whilst using blocks is similar in some respects to strata, blocks are never permuted so if you can use blocks you can use strata as you won't be permuting them.
If you want to permute the years as groups of samples, then you'll need strata and you'll need balance at the year level, which you don't have.
What you have defined with your call to how() is:
groups samples by year and as such samples will never be swapped between years, and
samples within the levels of year will be permuted in series, keeping their temporal order intact after applying cyclic shift permutations.
If that's not what you want to do, you need to explain in words what you want to do. By "do" I mean what is it you want to test? What is your model in vegan?

Related

Reducing "treatment" sample size through MatchIt (or another package) to increase sample similarity

I am trying to match two samples on several covariates using MatchIt, but I am having difficulty creating samples that are similar enough. Both my samples are plenty large (~1000 in the control group, ~5000 in the comparison group).
I want to get a matched sample with participants as closely matched as possible and I am alright with losing sample size in the control group. Right now, MatchIt only returns two groups of 1000, whereas I want two groups that are very closely matched and would be fine with smaller groups (e.g., 500 instead of 1000).
Is there a way to do this through either MatchIt or another package? I would rather avoid using random sampling and then match if possible because I want as close a match between groups as possible.
Apologies for not having a reproducible example, I am still pretty new to using R and couldn't figure out how to make a sample of this issue...
Below is the code I have for matching the two groups.
data<- na.omit(data)
data$Group<- as.numeric(data$Group)
data$Group<- recode(data$Group, '1 = 1; 2 = 0')
m.out <- matchit(Group ~ Age + YearsEdu + Income + Gender, data = data, ratio = 1)
s.out <- summary(m.out, standardize = TRUE)
plot(s.out)
matched.data <- match.data(m.out)
MatchIt, like other similar packages, offers several matching routines that enable you to play around with the settings. Check out the argument method, which is set to method = 'nearest' by default. This means that unless you specify, it will look for the best match for each of the treatment observations. In your case, you will always have 1000 paired matches with this setting.
You can choose to set it to method = 'exact', which is much more restrictive. In the documentation you will find:
This technique matches each treated unit to all
possible control units with exactly the same values on all the covariates, forming subclasses
such that within each subclass all units (treatment and control) have the same covariate values.
On the lalonde dataset, you can run:
m.out <- matchit(treat ~ educ + black + hispan, data = lalonde, method = 'exact')
summary(m.out)
As a consequence, it discards some of the treatment observation that could not get matched. Have a look at the other possibilities for method, maybe you will find something you will like better.
That being said, be mindful not to discard too many treatment observations. If you do, you will make the treatment group look like the control group (instead of the opposite), which might lead to unwanted results.
You should look into the package designmatch, which implements a form of matching called cardinality matching that does what you want (i.e., find the largest matched set that yields desired balance). Unlike MatchIt, designmatch doesn't use a distance variable; instead, it uses optimization to solve the matching problem. You select exactly how balanced you want each covariate to be, and it will do its best to solve the problem while retaining as many matches as possible. The methodology is described in Zubizarreta, Paredes, & Rosenbaum (2014).

How to understand RandomForestExplainer output (R package)

I have the following code, which basically try to predict the Species from iris data using randomForest. What I'm really intersed in is to find what are the best features (variable) that explain the species classification. I found the package randomForestExplainer is the best
to serve the purpose.
library(randomForest)
library(randomForestExplainer)
forest <- randomForest::randomForest(Species ~ ., data = iris, localImp = TRUE)
importance_frame <- randomForestExplainer::measure_importance(forest)
randomForestExplainer::plot_multi_way_importance(importance_frame, size_measure = "no_of_nodes")
The result of the code produce this plot:
Based on the plot, the key factor to explain why Petal.Length and Petal.Width is the best factor are these (the explanation is based on the vignette):
mean_min_depth – mean minimal depth calculated in one of three ways specified by the parameter mean_sample,
times_a_root – total number of trees in which Xj is used for splitting the root node (i.e., the whole sample is divided into two based on the value of Xj),
no_of_nodes – total number of nodes that use Xj for splitting (it is usually equal to no_of_trees if trees are shallow),
It's not entirely clear to me why the high times_a_root and no_of_nodes is better? And low mean_min_depth is better?
What are the intuitive explanation for that?
The vignette information doesn't help.
You would like a statistical model or measure to be a balance between "power" and "parsimony". The randomForest is designed internally to do penalization as its statistical strategy for achieving parsimony. Furthermore the number of variables selected in any given sample will be less than the the total number of predictors. This allows model building when hte number of predictors exceeds the number of cases (rows) in the dataset. Early splitting or classification rules can be applied relatively easily, but subsequent splits become increasingly difficult to meet criteria of validity. "Power" is the ability to correctly classify items that were not in the subsample, for which a proxy, the so-called OOB or "out-of-bag" items is used. The randomForest strategy is to do this many times to build up a representative set of rules that classify items under the assumptions that the out-of-bag samples will be a fair representation of the "universe" from which the whole dataset arose.
The times_a_root would fall into the category of measuring the "relative power" of a variable compared to its "competitors". The times_a_root statistic measures the number of times a variable is "at the top" of a decision tree, i.e., how likely it is to be chosen first in the process of selecting split criteria. The no_of_node measures the number of times the variable is chosen at all as a splitting criterion among all of the subsampled.
From:
?randomForest # to find the names of the object leaves
forest$ntree
[1] 500
... we can see get a denominator for assessing the meaning of the roughly 200 values in the y-axis of the plot. About 2/5ths of the sample regressions had Petal.Length in the top split criterion, while another 2/5ths had Petal.Width as the top variable selected as the most important variable. About 75 of 500 had Sepal.Length while only about 8 or 9 had Sepal.Width (... it's a log scale.) In the case of the iris dataset, the subsamples would have ignored at least one of the variables in each subsample, so the maximum possible value of times_a_root would have been less than 500. Scores of 200 are pretty good in this situation and we can see that both of these variables have a comparable explanatory ability.
The no_of_nodes statistic totals up the total number of trees that had that variable in any of its nodes, remembering that the number of nodes would be constrained by the penalization rules.

Apply k-means to examine differences between two groups in R

I have two groups. The treatment group is exposure to media; the control group is no media. They are distinguished by a categorial variable in the data frame. (exposure to media = 1, no media = 0)
Now, I want to examine whether there are any clear differences between these two groups. To do this, apply the k-means algorithm with two clusters to four variables (proportion of black population, proportion of male population, proportion of hispanic population, median income on the logarithmic scale).
How to do this in R? Could anyone give some hints? Thanks!
Try this:
km <-kmeans(your data, 2, nstart=10)
your data here as a data.frame (your whole data or you can select the variables that you are interesting about them). You need to select the number of clusters (here is 2). A good practice to understand your data is to apply different number of cluster and then see which one fit your data better (use for example any criteria methods such as AIC or BIC).
k-means is an approach applied to cluster data. Where this data come from different distribution and we would like to know from where each observation come from (from which distribution).
You can also have a look at many tutorials about kmeans in R. For example,
https://onlinecourses.science.psu.edu/stat857/node/125
https://www.r-statistics.com/2013/08/k-means-clustering-from-r-in-action/
http://www.statmethods.net/advstats/cluster.html

How to specify subset/ sample number for permutations using specaccum() in R's vegan package

I have a community matrix (species as columns, samples as rows) from which I would like to generate a species accumulation curve (SAC) using the specaccum() and fitspecaccum() functions in R's vegan package. In order for the resulting SAC and cumulative species richness at sample X to be comparable among regions (I have 1 community matrix per region), I need to have specaccum() choose the same number of sets within each region. My problem is that some regions have a larger number of sets than others. I would like to limit the sample size to the minimum number of sets among regions (in my case, the minimum number of sets is 45, so I would like specaccum() to randomly sample 45 sets, 100 times (set permutations=100) for each region. I would like to sample from the entire data set available for each region. The code below has not worked... it doesn't recognize "subset=45". The vegan package info says "subset" needs to be logical... I don't understand how subset number can be logical, but maybe I am misinterpreting what subset is... Is there another way to do this? Would it be sufficient to run specaccum() for the entire number of sets available for each region and then just truncate the output to 45?
require(vegan)
pool1<-specaccum(comm.matrix, gamma="jack1", method="random", subet=45, permutations=100)
Any help is much appreciated.
Why do you want to limit the function to work in a random sample of 45 cases? Just use the species accumulation up to 45 cases. Taking a random subset of 45 cases gives you the same accumulation, except for the random error of subsampling and throwing away information. If you want to compare your different cases, just compare them at the sample size that suits all cases, that is, at 45 or less. That is the idea of species accumulation models.
The subset is intended for situations where you have (possibly) heterogeneous collection of sampling units, and you want to stratify data. For instance, if you want to see only the species accumulation in the "OldLow" habitat type of the Barro Colorado data, you could do:
data(BCI, BCI.env)
plot(specaccum(BCI, subset = BCI.env$Habitat == "OldLow"))
If you want to have, say, a subset of 30 sample plots of the same data, you could do:
take <- c(rep(TRUE, 30), rep(FALSE, 20))
plot(specaccum(BCI)) # to see it all
# repeat the following to see how taking subset influences
lines(specaccum(BCI, subset = sample(take)), col = "blue")
If you repeat the last line, you see how taking random subset influences the results: the lines are normally within the error bars of all data, but differ from each other due to random error.

Is it possible to specify a range for numbers randomly generated by mvrnorm( ) in R?

I am trying to generate a random set of numbers that exactly mirror a data set that I have (to test it). The dataset consists of 5 variables that are all correlated with different means and standard deviations as well as ranges (they are likert scales added together to form 1 variable). I have been able to get mvrnorm from the MASS package to create a dataset that replicated the correlation matrix with the observed number of observations (after 500,000+ iterations), and I can easily reassign means and std. dev. through z-score transformation, but I still have specific values within each variable vector that are far above or below the possible range of the scale whose score I wish to replicate.
Any suggestions how to fix the range appropriately?
Thank you for sharing your knowledge!
To generate a sample that does "exactly mirror" the original dataset, you need to make sure that the marginal distributions and the dependence structure of the sample matches those of the original dataset.
A simple way to achieve this is with resampling
my.data <- matrix(runif(1000, -1, 2), nrow = 200, ncol = 5) # Some dummy data
my.ind <- sample(1:nrow(my.data), nrow(my.data), replace = TRUE)
my.sample <- my.data[my.ind, ]
This will ensure that the margins and the dependence structure of the sample (closely) matches those of the original data.
An alternative is to use a parametric model for the margins and/or the dependence structure (copula). But as staded by #dickoa, this will require serious modeling effort.
Note that by using a multivariate normal distribution, you are (implicity) assuming that the dependence structure of the original data is the Gaussian copula. This is a strong assumption, and it would need to be validated beforehand.

Resources