Make multiple histograms at once and save them - r

I would like to make histogram of columns 5-34 of my data set and save them for reference. This is what I have and the error 'x must be numeric' is what keeps coming up. All of these columns have numeric data.
[data screenshot][1]
dput(longbroca)
histograms = c()
GBhistograms = c()
RThistograms = c()
for (i in 5:34){
hist(longbroca)
hist(GBlongbroca)
hist(RTlongbroca)
histograms = c(histograms, hist(longbroca[,5:34]))
GBhistograms = c(GBhistograms, hist(GBlongbroca[,5:34]))
RThistograms = c(RThistograms, hist(RTlongbroca[,5:34]))
}
#reproducible
fakerow1 <- c(100,80,60,40,20)
fakerow2 <- c(100,80,60,40,20)
fakedata = rbind(fakerow1,fakerow2)
colnames(fakedata) = c('ant1','ant2','ant3','ant4','ant5')

You cannot plot all of the columns with a single hist() function. That is why you are getting the error message. You are plotting histograms and saving the output list from the histogram. Your code does not save any histograms, only the data for producing them. If you actually want to save the plotted histograms, you need to plot them to a device (e.g. pdf).
We can use the iris dataset which comes with R (data(iris)) as some example data. The first 4 columns are numeric. If you just want the data for the histograms from the iris data set (columns 1 through 4):
# R will plot all four but you will only see the last one.
histograms <- lapply(iris[, 1:4], hist)
The variable histograms is a list that contains 6 elements. These are documented on the manual page for the function (?hist).
# To plot one of the histograms with a title and x-axis label:
lbl <- names(histograms)
plot(histograms[[1]], main=lbl[1], xlab=lbl[1])
# To plot them all
pdf("histograms.pdf")
lapply(1:4, function(x) plot(histograms[[x]], main=lbl[x], xlab=lbl[x]))
dev.off()
The file "histograms.pdf" will have all four histograms, one per page.

Related

Set common y axis limits from a list of ggplots

I am running a function that returns a custom ggplot from an input data (it is in fact a plot with several layers on it). I run the function over several different input data and obtain a list of ggplots.
I want to create a grid with these plots to compare them but they all have different y axes.
I guess what I have to do is extract the maximum and minimum y axes limits from the ggplot list and apply those to each plot in the list.
How can I do that? I guess its through the use of ggbuild. Something like this:
test = ggplot_build(plot_list[[1]])
> test$layout$panel_scales_x
[[1]]
<ScaleContinuousPosition>
Range:
Limits: 0 -- 1
I am not familiar with the structure of a ggplot_build and maybe this one in particular is not a standard one as it comes from a "custom" ggplot.
For reference, these plots are created whit the gseaplot2 function from the enrichplot package.
I dont know how to "upload" an R object but if that would help, let me know how to do it.
Thanks!
edit after comments (thanks for your suggestions!)
Here is an example of the a gseaplot2 plot. GSEA stands for Gene Set Enrichment Analysis, it is a technique used in genomic studies. The gseaplot2 function calculates a running average and then plots it and another bar plot on the bottom.
and here is the grid I create to compare the plots generated from different data:
I would like to have a common scale for the "Running Enrichment Score" part.
I guess I could try to recreate the gseaplot2 function and input all of the datasets and then create the grid by facet_wrap, but I was wondering if there was an easy way of extracting parameters from a plot list.
As a reproducible example (from the enrichplot package):
library(clusterProfiler)
data(geneList, package="DOSE")
gene <- names(geneList)[abs(geneList) > 2]
wpgmtfile <- system.file("extdata/wikipathways-20180810-gmt-Homo_sapiens.gmt", package="clusterProfiler")
wp2gene <- read.gmt(wpgmtfile)
wp2gene <- wp2gene %>% tidyr::separate(term, c("name","version","wpid","org"), "%")
wpid2gene <- wp2gene %>% dplyr::select(wpid, gene) #TERM2GENE
wpid2name <- wp2gene %>% dplyr::select(wpid, name) #TERM2NAME
ewp2 <- GSEA(geneList, TERM2GENE = wpid2gene, TERM2NAME = wpid2name, verbose=FALSE)
gseaplot2(ewp2, geneSetID=1, subplots=1:2)
And this is how I generate the plot list (probably there is a much more elegant way):
plot_list = list()
for(i in 1:3) {
fig_i = gseaplot2(ewp2,
geneSetID=i,
subplots=1:2)
plot_list[[i]] = fig_i
}
ggarrange(plotlist=plot_list)

How to adjust x labels in R boxplot

This is my code to create a boxplot in R that has 4 boxplots in one.
psnr_x265_256 <- c(39.998,39.998, 40.766, 38.507,38.224,40.666,38.329,40.218,44.746,38.222)
psnr_x264_256 <- c(39.653, 38.106,37.794,36.13,36.808,41.991,36.718,39.26,46.071,36.677)
psnr_xvid_256 <- c(33.04564,33.207269,32.715427,32.104696,30.445141,33.135261,32.669766, 31.657039,31.53103,31.585865)
psnr_mpeg2_256 <- c(32.4198,32.055051,31.424819,30.560274,30.740421,32.484694, 32.512268,32.04659,32.345848, 31)
all_errors = cbind(psnr_x265_256, psnr_x264_256, psnr_xvid_256,psnr_mpeg2_256)
modes = cbind(rep("PSNR",10))
journal_linear_data <-data.frame(psnr_x265_256, psnr_x264_256, psnr_xvid_256,psnr_mpeg2_256)
yvars <- c("psnr_x265_256","psnr_x264_256","psnr_xvid_256","psnr_mpeg2_256")
xvars <- c("x265","x264","xvid","mpeg2")
bmp(filename="boxplot_PSNR_256.bmp")
boxplot(journal_linear_data[,yvars], xlab=xvars, ylab="PSNR")
dev.off()
This is the image I get.
I want to have the corresponding values for each boxplot in x axis "x265","x264","xvid","mpeg2".
Do you have any idea how to fix this?
There are multiple ways of changing the labels for your boxplot variables. Probably the simplest way is changing the column names of your data frame:
colnames(journal_linear_data) <- c("x265","x264","xvid","mpeg2")
Even simpler: you could do this right at the creation of your data frame too:
journal_linear_data <- data.frame(x265=psnr_x265_256, x264=psnr_x264_256, xvid=psnr_xvid_256, mpeg2=psnr_mpeg2_256)
If you run into the problem of your labels not being shown or overlapping due to too few space, try rotating the x labels using the las parameter, e.g. las=2 or las=3.

How can I extract the matrix derived from a heatmap created with gplots after hierarchical clustering?

I am making a heatmap, but I can't assign the result in a variable to check the result before plotting. Rstudio plot it automatically. I would like to get the list of rownames in the order of the heatmap. I'am not sure if this is possible. I'am using this code:
hm <- heatmap.2( assay(vsd)[ topVarGenes, ], scale="row",
trace="none", dendrogram="both",
col = colorRampPalette( rev(brewer.pal(9, "RdBu")) )(255),
ColSideColors = c(Controle="gray", Col1.7G2="darkgreen", JG="blue", Mix="orange")[
colData(vsd)$condition ] )
You can assign the plot to an object. The plot will still be drawn in the plot window, however, you'll also get a list with all the data for each plot element. Then you just need to extract the desired plot elements from the list. For example:
library(gplots)
p = heatmap.2(as.matrix(mtcars), dendrogram="both", scale="row")
p is a list with all the elements of the plot.
p # Outputs all the data in the list; lots of output to the console
str(p) # Struture of p; also lots of output to the console
names(p) # Names of all the list elements
p$rowInd # Ordering of the data rows
p$carpet # The heatmap values
You'll see all the other values associated with the dendrogram and the heatmap if you explore the list elements.
To others out there, a more complete description way to capture a matrix representation of the heatmap created by gplots:
matrix_map <- p$carpet
matrix_map <- t(matrix_map)

Issue: ggplot2 replicates last plot of a list in grid

I have some 16 plots. I want to plot all of these in grid manner with ggplot2. But, whenever I plot, I get a grid with all the plots same, i.e, last plot saved in a list gets plotted at all the 16 places of grid. To replicate the same issue, here I am providing a simple example with two files. Although data are entirely different, but plots drawn are similar.
library(ggplot2)
library(grid)
library(gridExtra)
library(scales)
set.seed(1006)
date1<- as.POSIXct(seq(from=1443709107,by=3600,to=1446214707),origin="1970-01-01")
power <- rnorm(length(date1),100,5)#with normal distribution
write.csv(data.frame(date1,power),"file1.csv",row.names = FALSE,quote = FALSE)
# Now another dataset with uniform distribution
write.csv(data.frame(date1,power=runif(length(date1))),"file2.csv",row.names = FALSE,quote = FALSE)
path=getwd()
files=list.files(path,pattern="*.csv")
plist<-list()# for saving intermediate ggplots
for(i in 1:length(files))
{
dframe<-read.csv(paste(path,"/",files[i],sep = ""),head=TRUE,sep=",")
dframe$date1= as.POSIXct(dframe$date1)
plist[[i]]<- ggplot(dframe)+aes(dframe$date1,dframe$power)+geom_line()
}
grid.arrange(plist[[1]],plist[[2]],ncol = 1,nrow=2)
You need to remove the dframe from your call to aes. You should do that anyway because you have provided a data-argument. In this case it's even more important because while you save the ggplot-object, things don't get evaluated until the call to plot/grid.arrange. When you do that, it looks at the current value of dframe, which is the last dataset in your iteration.
You need to plot with:
ggplot(dframe)+aes(date1,power)+geom_line()

Assigning "beanplot" object to variable in R

I have found that the beanplot is the best way to represent my data. I want to look at multiple beanplots together to visualize my data. Each of my plots contains 3 variables, so each one looks something like what would be generated by this code:
library(beanplot)
a <- rnorm(100)
b <- rnorm(100)
c <- rnorm(100)
beanplot(a, b ,c ,ylim = c(-4, 4), main = "Beanplot",
col = c("#CAB2D6", "#33A02C", "#B2DF8A"), border = "#CAB2D6")
(Would have just included an image but my reputation score is not high enough, sorry)
I have 421 of these that I want to put into one long PDF (EDIT: One plot per page is fine, this was just poor wording on my part). The approach I have taken was to first generate the beanplots in a for loop and store them in a list at each iteration. Then I will use the multiplot function (from the R Cookbook page on multiplot) to display all of my plots on one long column so I can begin my analysis.
The problem is that the beanplot function does not appear to be set up to assign plot objects as a variable. Example:
library(beanplot)
a <- rnorm(100)
b <- rnorm(100)
plot1 <- beanplot(a, b, ylim = c(-5,5), main = "Beanplot",
col = c("#CAB2D6", "#33A02C", "#B2DF8A"), border = "#CAB2D6")
plot1
If you then type plot1 into the R console, you will get back two of the plot parameters but not the plot itself. This means that when I store the plots in the list, I am unable to graph them with multiplot. It will simply return the plot parameters and a blank plot.
This behavior does not seem to be the case with qplot for example which will return a plot when you recall the stored plot. Example:
library(ggplot2)
a <- rnorm(100)
b <- rnorm(100)
plot2 <- qplot(a,b)
plot2
There is no equivalent to the beanplot that I know of in ggplot. Is there some sort of workaround I can use for this issue?
Thank you.
You can simply open a PDF device with pdf() and keep the default parameter onefile=TRUE. Then call all your beanplot()s, one after the other. They will all be in one PDF document, each one on a separate page. See here.

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