I'm currently performing a multiple sequence alignment using the 'msa' package from Bioconductor. I'm using this to calculate the consensus sequence (msaConsensusSequence) and conservation score (msaConservationScore). This gives me outputs that are values ...
e.g.
ConsensusSequence:
i.llE etc (str = chr)
(lower case = 20%+ conservation, uppercase = 80%+ conservation, . = <20% conservation)
ConservationScore:
221 -296 579 71 423 etc (str = named num)
I would like to convert these into a table where the first row contains columns where each is a different letter in the consensus sequence and the second row is the corresponding conservation score.
e.g.
i . l l E
221 -296 579 71 423
Could people please advise on the best way to go about this?
Thanks
Natalie
For what you have said in the comments you can get a data frame like this:
data(BLOSUM62)
alignment <- msa(mySequences)
conservation <- msaConservationScore(alignment, BLOSUM62)
# Now create the data fram
df <- data.frame(consensus = names(conservation), conservation = conservation)
head(df)
consensus conservation
1 T 141
2 E 160
3 E 165
4 E 325
5 ? 179
6 ? 71
7 T 216
8 W 891
9 ? 38
10 T 405
11 L 204
If you prefer to transpose it you can:
df <- t(df)
colnames(df) <- 1:ncol(df)
Related
I am looking to workout a percentage total over a look back range in R.
I know how to do this in excel with the following formula:
=SUM(B2:B4)/SUM(B2:B4,C2:C4)
This is summing column B over a range of today looking back 3 lines. It then divides this sum buy the total sum of column B + C again looking back 3 lines.
I am looking to achieve the same calculation in R to run across my matrix.
The output would look something like this:
adv dec perct
1 69 376
2 113 293
3 270 150 0.355625492
4 74 371 0.359559402
5 308 96 0.513790386
6 236 173 0.491255962
7 252 134 0.663886572
8 287 129 0.639966969
9 219 187 0.627483444
This is a line of code I could perhaps add the look back range too:
perct <- apply(data.matrix[,c('adv','dec')], 1, function(x) { (x[1] / x[1] + x[2]) } )
If i could get [1] to sum the previous 3 line range and
If i could get [2] to also sum the previous 3 line range.
Still learning how to apply forward and look back periods within R. So any additional learning on the answer would be appreciated!
Here are some approaches. The first 3 use rollsumr and/or rollapplyr in zoo and the last one uses only the base of R.
1) rollsumr Create a matrix with rollsumr whose columns contain the rollling sums, convert that to row proportions and take the "adv" column. Finally assign that to a new column frac in DF. This approach has the shortest code.
library(zoo)
DF$frac <- prop.table(rollsumr(DF, 3, fill = NA), 1)[, "adv"]
giving:
> DF
adv dec frac
1 69 376 NA
2 113 293 NA
3 270 150 0.3556255
4 74 371 0.3595594
5 308 96 0.5137904
6 236 173 0.4912560
7 252 134 0.6638866
8 287 129 0.6399670
9 219 187 0.6274834
1a) This variation is similar except instead of using prop.table we write out the ratio. The code is longer but you may find it clearer.
m <- rollsumr(DF, 3, fill = NA)
DF$frac <- with(as.data.frame(m), adv / (adv + dec))
1b) This is a variation of (1) that is the same except it uses a magrittr pipeline:
library(magrittr)
DF %>% rollsumr(3, fill = NA) %>% prop.table(1) %>% `[`(TRUE, "adv") -> DF$frac
2) rollapplyr We could use rollapplyr with by.column = FALSE like this. The result is the same.
ratio <- function(x) sum(x[, "adv"]) / sum(x)
DF$frac <- rollapplyr(DF, 3, ratio, by.column = FALSE, fill = NA)
3) Yet another variation is to compute the numerator and denominator separately:
DF$frac <- rollsumr(DF$adv, 3, fill = NA) /
rollapplyr(DF, 3, sum, by.column = FALSE, fill = NA)
4) base This uses embed followed by rowSums on each column to get the rolling sums and then uses prop.table as in (1).
DF$frac <- prop.table(sapply(lapply(rbind(NA, NA, DF), embed, 3), rowSums), 1)[, "adv"]
Note: The input used in reproducible form is:
Lines <- "adv dec
1 69 376
2 113 293
3 270 150
4 74 371
5 308 96
6 236 173
7 252 134
8 287 129
9 219 187"
DF <- read.table(text = Lines, header = TRUE)
Consider an sapply that loops through the number of rows in order to index two rows back:
DF$pred <- sapply(seq(nrow(DF)), function(i)
ifelse(i>=3, sum(DF$adv[(i-2):i])/(sum(DF$adv[(i-2):i]) + sum(DF$dec[(i-2):i])), NA))
DF
# adv dec pred
# 1 69 376 NA
# 2 113 293 NA
# 3 270 150 0.3556255
# 4 74 371 0.3595594
# 5 308 96 0.5137904
# 6 236 173 0.4912560
# 7 252 134 0.6638866
# 8 287 129 0.6399670
# 9 219 187 0.6274834
I have a data set where I have the Levels and Trends for say 50 cities for 3 scenarios. Below is the sample data -
City <- paste0("City",1:50)
L1 <- sample(100:500,50,replace = T)
L2 <- sample(100:500,50,replace = T)
L3 <- sample(100:500,50,replace = T)
T1 <- runif(50,0,3)
T2 <- runif(50,0,3)
T3 <- runif(50,0,3)
df <- data.frame(City,L1,L2,L3,T1,T2,T3)
Now, across the 3 scenarios I find the minimum Level and Minimum Trend using the below code -
df$L_min <- apply(df[,2:4],1,min)
df$T_min <- apply(df[,5:7],1,min)
Now I want to check if these minimum values are significantly different between the levels and trends respectively. So check L_min with columns 2-4 and T_min with columns 5-7. This needs to be done for each city (row) and if significant then return which column it is significantly different with.
It would help if some one could guide how this can be done.
Thank you!!
I'll put my idea here, nevertheless I'm looking forward for ideas for others.
> head(df)
City L1 L2 L3 T1 T2 T3 L_min T_min
1 City1 251 176 263 1.162313 0.07196579 2.0925715 176 0.07196579
2 City2 385 406 264 0.353124 0.66089524 2.5613980 264 0.35312402
3 City3 437 333 426 2.625795 1.43547766 1.7667891 333 1.43547766
4 City4 431 405 493 2.042905 0.93041254 1.3872058 405 0.93041254
5 City5 101 429 100 1.731004 2.89794314 0.3535423 100 0.35354230
6 City6 374 394 465 1.854794 0.57909775 2.7485841 374 0.57909775
> df$FC <- rowMeans(df[,2:4])/df[,8]
> df <- df[order(-df$FC), ]
> head(df)
City L1 L2 L3 T1 T2 T3 L_min T_min FC
18 City18 461 425 117 2.7786757 2.6577894 0.75974121 117 0.75974121 2.857550
38 City38 370 117 445 0.1103141 2.6890014 2.26174542 117 0.11031411 2.655271
44 City44 101 473 222 1.2754675 0.8667007 0.04057544 101 0.04057544 2.627063
10 City10 459 361 132 0.1529519 2.4678493 2.23373484 132 0.15295194 2.404040
16 City16 232 393 110 0.8628494 1.3995549 1.01689217 110 0.86284938 2.227273
15 City15 499 475 182 0.3679611 0.2519497 2.82647041 182 0.25194969 2.117216
Now you have the most different rows based on columns 2:4 at the top. Columns 5:7 in analogous way.
And some tips for stastical tests:
Always use t.test(parametrical, based on mean) instead of wilcoxon(u-mann whitney - non-parametrical, based on median), it has more power; HOWEVER:
-Data sets should be big ex. hipotesis: Montreal has taller citizens than Quebec; t.test will work fine when you take a 100 people from each city, so we have height measurment of 200 people 100 vs 100.
-Distribution should be close to normal distribution in all samples; or both samples should have similar distribution far from normal - it may be binominal. Anyway we can't use this test when one sample has normal distribution, and second hasn't.
-Size of both samples should be eqal, so 100 vs 100 is ok, but 87 vs 234 not exactly, p-value will be below 0.05, however it may be misrepresented.
If your data doesn't meet above conditions, I prefer non-parametrical test, less power but more resistant.
I would like to understand how to subset multiple columns from same data frame by matching the first 5 letters of the column names with each other and if they are equal then subset it and store it in a new variable.
Here is a small explanation of my required output. It is described below,
Lets say the data frame is eatable
fruits_area fruits_production vegetable_area vegetable_production
12 100 26 324
33 250 40 580
66 510 43 581
eatable <- data.frame(c(12,33,660),c(100,250,510),c(26,40,43),c(324,580,581))
names(eatable) <- c("fruits_area", "fruits_production", "vegetables_area",
"vegetable_production")
I was trying to write a function which will match the strings in a loop and will store the subset columns after matching first 5 letters from the column names.
checkExpression <- function(dataset,str){
dataset[grepl((str),names(dataset),ignore.case = TRUE)]
}
checkExpression(eatable,"your_string")
The above function checks the string correctly but I am confused how to do matching among the column names in the dataset.
Edit:- I think regular expressions would work here.
You could try:
v <- unique(substr(names(eatable), 0, 5))
lapply(v, function(x) eatable[grepl(x, names(eatable))])
Or using map() + select_()
library(tidyverse)
map(v, ~select_(eatable, ~matches(.)))
Which gives:
#[[1]]
# fruits_area fruits_production
#1 12 100
#2 33 250
#3 660 510
#
#[[2]]
# vegetables_area vegetable_production
#1 26 324
#2 40 580
#3 43 581
Should you want to make it into a function:
checkExpression <- function(df, l = 5) {
v <- unique(substr(names(df), 0, l))
lapply(v, function(x) df[grepl(x, names(df))])
}
Then simply use:
checkExpression(eatable, 5)
I believe this may address your needs:
checkExpression <- function(dataset,str){
cols <- grepl(paste0("^",str),colnames(dataset),ignore.case = TRUE)
subset(dataset,select=colnames(dataset)[cols])
}
Note the addition of "^" to the pattern used in grepl.
Using your data:
checkExpression(eatable,"fruit")
## fruits_area fruits_production
##1 12 100
##2 33 250
##3 660 510
checkExpression(eatable,"veget")
## vegetables_area vegetable_production
##1 26 324
##2 40 580
##3 43 581
Your function does exactly what you want but there was a small error:
checkExpression <- function(dataset,str){
dataset[grepl((str),names(dataset),ignore.case = TRUE)]
}
Change the name of the object from which your subsetting from obje to dataset.
checkExpression(eatable,"fr")
# fruits_area fruits_production
#1 12 100
#2 33 250
#3 660 510
checkExpression(eatable,"veg")
# vegetables_area vegetable_production
#1 26 324
#2 40 580
#3 43 581
i have such a difficult question (at least to me) that i spend 2 hours just writing it. Complete impossible to program it by my self. I try to be very clear and i´m sorry if i didn´t. I´m doing this in a very rustic way in excel, but i really need to program this.
i have a data.frame like this
id_pix id_lote clase f1 f2
45 4 Sg 2460 2401
46 4 Sg 2620 2422
47 4 Sg 2904 2627
48 5 M 2134 2044
49 5 M 2180 2104
50 5 M 2127 2069
83 11 S 2124 2062
84 11 S 2189 2336
85 11 S 2235 2162
86 11 S 2162 2153
87 11 S 2108 2124
with 17451 "id_pixel"(rows), 2080 "id_lote" and 9 "clase"
this is the "id_lote" count per "clase" (v1 is the id_lote count)
clase v1
1: S 1099
2: P 213
3: Sg 114
4: M 302
5: Alg 27
6: Az 77
7: Po 228
8: Cit 13
9: Ma 7
i need to split the "id_lote" randomly within the "clase". I mean i have 1099 "id_lote" for the "S" "clase" that are 9339 "id_pixel" (rows) and i want to randomly select 50 % of "id_lote" that are x "id_pixel"(rows). And do this for every "clase" considering that the size (number of "id_lote") of every "clase" are different. I also would like to be able to change the size of the selection (50 %, 30 %, etc). And i also want to keep the not selected set of "id_lote". I hope some one can help me with this!
here is the reproducible example
this is the data with 2 clase (S and Az), with 6 id_lote and 13 id_pixel
id_pix id_lote clase f1 f2
1 1 S 2909 2381
2 1 S 2515 2663
3 1 S 2628 3249
30 2 S 3021 2985
31 2 S 3020 2596
71 9 S 4725 4404
72 9 S 4759 4943
75 11 S 2728 2225
218 21 Az 4830 3007
219 21 Az 4574 2761
220 21 Az 5441 3092
1155 126 Az 7209 2449
1156 126 Az 7035 2932
and one result could be:
id_pix id_lote clase f1 f2
1 1 S 2909 2381
2 1 S 2515 2663
3 1 S 2628 3249
75 11 S 2728 2225
1155 126 Az 7209 2449
1156 126 Az 7035 2932
were 50% of id_lote were randomly selected in clase "S" (2 of 4 id_lote) but all the id_pixel in selected id_lote were keeped. The same for clase "Az", one id_lote was randomly selected (1 of 2 in this case) and all the id_pixel in selected id_lote were keeped.
what colemand77 proposed helped a lot. I think dplyr package is usefull for this but i think that if i do
df %>%
group_by(clase, id_lote) %>%
sample_frac(.3, replace = FALSE)
i get the 30 % of the data of each clase but not grouped by id_lote like i need! I mean 30 % of the rows (id_pixel) were selected instead of id_lote.
i hope this example help to understand what i want to do and make it usefull for everybody. I´m sorry if i wasn´t clear enough the first time.
Thanks a lot!
First glimpse I'd say the dplyr package is your friend here.
df %>%
group_by(clase, id_lote) %>%
sample_frac(.3, replace = FALSE)
so you first use group_by() and include the grouping levels you want to sample from, then you use sample_frac to sample the fraction of the results you want for each group.
As near as I can tell this is what you are asking for. If not, please consider re-stating your question to include either a reproducible example or clarify. Cheers.
to "keep" the not-selected members, I would add a column of unique ids, and use an anti-join anti_join()(also from the dplyr package) to find the id's that are not in common between the two data.frames (the results of the sampling and the original).
## Update ##
I'm understanding better now, I believe. Think about this as a two step process...
1) you want to select x% (50 in example) of the id_lote from each clase and return those id_lote #s (i'm assuming that a given id_lote does not exist for multiple clase?)
2) you want to see all of the id_pixels that correspond to each id_lote, all in one data.frame
I've broken this down into multiple steps for illustration, not because it is the fastest / prettiest.
raw data: (couldn't read your data into R.)
df<-data.frame(id_pix = c(1:200),
id_lote = sample(1:20,200, replace = TRUE),
clase = sample(letters[seq_along(1:10)], 200, replace = TRUE),
f1 = sample(1000:2000,200, replace = TRUE),
f2 = sample(2000:3000,200, replace = TRUE))
1) figure out which id_lote correspond to which clase - for this we use the dplyr summarise function and store it in a variable
summary<-df %>%
ungroup() %>%
group_by(clase, id_lote) %>%
summarise()
returns:
Source: local data frame [125 x 2]
Groups: clase
clase id_lote
1 a 1
2 a 2
3 a 4
4 a 5
5 a 6
6 a 7
7 a 8
8 a 9
9 a 11
10 a 12
.. ... ...
then we sample to get the 30% of the id_lote for each clase..
sampled_summary <- summary %>%
group_by(clase) %>%
sample_frac(.3,replace = FALSE)
so the result of this is a data table with two columns, (clase and id_lote) with 30% of the id_lotes shown for each clase.
2) ok so now we have the id_lotes randomly selected from each class but not the id_pix that are associated with that class. To accomplish this we do a join to get the corresponding full data set including the id_pix, etc.
result <- sampled_summary %>%
left_join(df)
The above makes a copy of the data set a bunch, so if you have a substantial data set you could just do it all at one go:
result <- df %>%
ungroup() %>%
group_by(clase, id_lote) %>%
summarise() %>%
group_by(clase) %>%
sample_frac(.5,replace = FALSE) %>%
left_join(df)
if this doesn't get you what you want, let me know and we'll take another crack at it.
I have a data frame having 20 columns. I need to filter / remove noise from one column. After filtering using convolve function I get a new vector of values. Many values in the original column become NA due to filtering process. The problem is that I need the whole table (for later analysis) with only those rows where the filtered column has values but I can't bind the filtered column to original table as the number of rows for both are different. Let me illustrate using the 'age' column in 'Orange' data set in R:
> head(Orange)
Tree age circumference
1 1 118 30
2 1 484 58
3 1 664 87
4 1 1004 115
5 1 1231 120
6 1 1372 142
Convolve filter used
smooth <- function (x, D, delta){
z <- exp(-abs(-D:D/delta))
r <- convolve (x, z, type='filter')/convolve(rep(1, length(x)),z,type='filter')
r <- head(tail(r, -D), -D)
r
}
Filtering the 'age' column
age2 <- smooth(Orange$age, 5,10)
data.frame(age2)
The number of rows for age column and age2 column are 35 and 15 respectively. The original dataset has 2 more columns and I like to work with them also. Now, I only need 15 rows of each column corresponding to the 15 rows of age2 column. The filter here removed first and last ten values from age column. How can I apply the filter in a way that I get truncated dataset with all columns and filtered rows?
You would need to figure out how the variables line up. If you can add NA's to age2 and then do Orange$age2 <- age2 followed by na.omit(Orange) you should have what you want. Or, equivalently, perhaps this is what you are looking for?
df <- tail(head(Orange, -10), -10) # chop off the first and last 10 observations
df$age2 <- age2
df
Tree age circumference age2
11 2 1004 156 915.1678
12 2 1231 172 876.1048
13 2 1372 203 841.3156
14 2 1582 203 911.0914
15 3 118 30 948.2045
16 3 484 51 1008.0198
17 3 664 75 955.0961
18 3 1004 108 915.1678
19 3 1231 115 876.1048
20 3 1372 139 841.3156
21 3 1582 140 911.0914
22 4 118 32 948.2045
23 4 484 62 1008.0198
24 4 664 112 955.0961
25 4 1004 167 915.1678
Edit: If you know the first and last x observations will be removed then the following works:
x <- 2
df <- tail(head(Orange, -x), -x) # chop off the first and last x observations
df$age2 <- age2