I'm trying to create a stacked barchart with gene sequencing data, where for each gene there is a tRF.type and Amino.Acid value. An example data set looks like this:
tRF <- c('tRF-26-OB1690PQR3E', 'tRF-27-OB1690PQR3P', 'tRF-30-MIF91SS2P46I')
tRF.type <- c('5-tRF', 'i-tRF', '3-tRF')
Amino.Acid <- c('Ser', 'Lys', 'Ser')
tRF.data <- data.frame(tRF, tRF.type, Amino.Acid)
I would like the x-axis to represent the amino acid type, the y-axis the number of counts of each tRF type and the the fill of the bars to represent each tRF type.
My code is:
ggplot(chart_data, aes(x = Amino.Acid, y = tRF.type, fill = tRF.type)) +
geom_bar(stat="identity") +
ggtitle("LAN5 - 4 days post CNTF treatment") +
xlab("Amino Acid") +
ylab("tRF type")
However, it generates this graph, where the y-axis is labelled with the categories of tRF type. How can I change my code so that the y-axis scale is numerical and represents the counts of each tRF type?
Barchart
OP and Welcome to SO. In future questions, please, be sure to provide a minimal reproducible example - meaning provide code, an image (if possible), and at least a representative dataset that can demonstrate your question or problem clearly.
TL;DR - don't use stat="identity", just use geom_bar() without providing a stat, since default is to use the counts. This should work:
ggplot(chart_data, aes(x = Amino.Acid, fill = tRF.type)) + geom_bar()
The dataset provided doesn't adequately demonstrate your issue, so here's one that can work. The example data herein consists of 100 observations and two columns: one called Capitals for randomly-selected uppercase letters and one Lowercase for randomly-selected lowercase letters.
library(ggplot2)
set.seed(1234)
df <- data.frame(
Capitals=sample(LETTERS, 100, replace=TRUE),
Lowercase=sample(letters, 100, replace=TRUE)
)
If I plot similar to your code, you can see the result:
ggplot(df, aes(x=Capitals, y=Lowercase, fill=Lowercase)) +
geom_bar(stat="identity")
You can see, the bars are stacked, but the y axis is all smooshed down. The reason is related to understanding the difference between geom_bar() and geom_col(). Checking the documentation for these functions, you can see that the main difference is that geom_col() will plot bars with heights equal to the y aesthetic, whereas geom_bar() plots by default according to stat="count". In fact, using geom_bar(stat="identity") is really just a complicated way of saying geom_col().
Since your y aesthetic is not numeric, ggplot still tries to treat the discrete levels numerically. It doesn't really work out well, and it's the reason why your axis gets smooshed down like that. What you want, is geom_bar(stat="count").... which is the same as just using geom_bar() without providing a stat=.
The one problem is that geom_bar() only accepts an x or a y aesthetic. This means you should only give it one of them. This fixes the issue and now you get the proper chart:
ggplot(df, aes(x=Capitals, fill=Lowercase)) + geom_bar()
You want your y-axis to be a count, not tRF.type. This code should give you the correct plot: I've removed the y = tRF.type from ggplot(), and stat = "identity from geom_bar() (it is using the default value of stat = "count instead).
ggplot(tRF.data, aes(x = Amino.Acid, fill = tRF.type)) +
geom_bar() +
ggtitle("LAN5 - 4 days post CNTF treatment") +
xlab("Amino Acid") +
ylab("tRF type")
Related
So I'm self-teaching myself R right now using this online resource: "https://r4ds.had.co.nz/data-visualisation.html#facets"
This particular section is going over the use of facet_wrap and facet_grid. It's clear to me that facet_grid is primarily used when wanting to visualize a plot along two additional dimensions, rather than just one. What I don't understand is why you can use facet_grid(.~variable) or facet_grid(variable~.) to basically achieve the same result as facet_wrap. Putting a "." in place of a variable results in just not faceting along the row or column dimension, or in other words showing 1 additional variable just as facet_wrap would do.
If anyone can shed some light on this, thank you!
If you use facet_grid, the facets will always be in one row/column. They will never wrap to make a rectangle. But really if you just have one variable with few levels, it doesn't much matter.
You can also see that facet_grid(.~variable) and facet_grid(variable~.) will put the facet labels in different places (row headings vs column headings)
mg <- ggplot(mtcars, aes(x = mpg, y = wt)) + geom_point()
mg + facet_grid(vs~ .) + labs(title="facet_grid(vs~ .)"),
mg + facet_grid(.~ vs) + labs(title="facet_grid(.~ vs)")
So in the most simple of cases, there's nothing that different between them. The main reason to use facet_grid is to have a single, common axis for all facets so you can easily scan across all panels to make a direct comparison of data.
Actually, the same result is not produced all the time...
The number of facets which appear across the graphs pane is fixed with facet_grid (always the number of unique values in the variable) where as facet_wrap, like its name suggests, wraps the facets around the graphics pane. In this way the functions only result in the same graph when the number of facets produced is small.
Both facet_grid and facet_wrap take their arguments in the form row~columns, and nowdays we don't need to use the dot with facet_grid.
In order to compare their differences let's add a new variable with 8 unqiue values to the mtcars data set:
library(tidyverse)
mtcars$example <- rep(1:8, length.out = 32)
ggplot()+
geom_point(data = mtcars, aes(x = mpg, y = wt))+
facet_grid(~example, labeller = label_both)
Which results in a cluttered plot:
Compared to:
ggplot()+
geom_point(data = mtcars, aes(x = mpg, y = wt))+
facet_wrap(~example, labeller = label_both)
Which results in:
I have used the following code to generate a plot with ggplot:
I want the legend to show the runs 1-8 and only the volumes 12.5 and 25 why doesn't it show it?
And is it possible to show all the points in the plot even though there is an overlap? Because right now the plot only shows 4 of 8 points due to overlap.
OP. You've already been given a part of your answer. Here's a solution given your additional comment and some explanation.
For reference, you were looking to:
Change a continuous variable to a discrete/discontinuous one and have that reflected in the legend.
Show runs 1-8 labeled in the legend
Disconnect lines based on some criteria in your dataset.
First, I'm representing your data here again in a way that is reproducible (and takes away the extra characters so you can follow along directly with all the code):
library(ggplot2)
mydata <- data.frame(
`Run`=c(1:8),
"Time"=c(834, 834, 584, 584, 1184, 1184, 938, 938),
`Area`=c(55.308, 55.308, 79.847, 79.847, 81.236, 81.236, 96.842, 96.842),
`Volume`=c(12.5, 12.5, 12.5, 12.5, 25.0, 25.0, 25.0, 25.0)
)
Changing to a Discrete Variable
If you check the variable type for each column (type str(mydata)), you'll see that mydata$Run is an int and the rest of the columns are num. Each column is understood to be a number, which is treated as if it were a continuous variable. When it comes time to plot the data, ggplot2 understands this to mean that since it is reasonable that values can exist between these (they are continuous), any representation in the form of a legend should be able to show that. For this reason, you get a continuous color scale instead of a discrete one.
To force ggplot2 to give you a discrete scale, you must make your data discrete and indicate it is a factor. You can either set your variable as a factor before plotting (ex: mydata$Run <- as.factor(mydata$Run), or use code inline, referring to aes(size = factor(Run),... instead of just aes(size = Run,....
Using reference to factor(Run) inline in your ggplot calls has the effect of changing the name of the variable to be "factor(Run)" in your legend, so you will have to also add that to the labs() object call. In the end, the plot code looks like this:
ggplot(data = mydata, aes(x=Area, y=Time)) +
geom_point(aes(color =as.factor(Volume), size = Run)) +
geom_line() +
labs(
x = "Area", y = "Time",
# This has to be changed now
color='Volume'
) +
theme_bw()
Note in the above code I am also not referring to mydata$Run, but just Run. It is greatly preferable that you refer to just the name of the column when using ggplot2. It works either way, but much better in practice.
Disconnect Lines
The reason your lines are connected throughout the data is because there's no information given to the geom_line() object other than the aesthetics of x= and y=. If you want to have separate lines, much like having separate colors or shapes of points, you need to supply an aesthetic to use as a basis for that. Since the two lines are different based on the variable Volume in your dataset, you want to use that... but keep the same color for both. For this, we use the group= aesthetic. It tells ggplot2 we want to draw a line for each piece of data that is grouped by that aesthetic.
ggplot(data = mydata, aes(x=Area, y=Time)) +
geom_point(aes(color =as.factor(Volume), size = Run)) +
geom_line(aes(group=as.factor(Volume))) +
labs(
x = "Area", y = "Time", color='Volume'
) +
theme_bw()
Show Runs 1-8 Labeled in Legend
Here I'm reading a bit into what you exactly wanted to do in terms of "showing runs 1-8" in the legend. This could mean one of two things, and I'll assume you want both and show you how to do both.
Listing and showing sizes 1-8 in the legend.
To set the values you see in the scale (legend) for size, you can refer to the various scale_ functions for all types of aesthetics. In this case, recall that since mydata$Run is an int, it is treated as a continuous scale. ggplot2 doesn't know how to draw a continuous scale for size, so the legend itself shows discrete sizes of points. This means we don't need to change Run to a factor, but what we do need is to indicate specifically we want to show in the legend all breaks in the sequence from 1 to 8. You can do this using scale_size_continuous(breaks=...).
ggplot(data = mydata, aes(x=Area, y=Time)) +
geom_point(aes(color =as.factor(Volume), size = Run)) +
geom_line(aes(group=as.factor(Volume))) +
labs(
x = "Area", y = "Time", color='Volume'
) +
scale_size_continuous(breaks=c(1:8)) +
theme_bw()
Showing all of your runs as points.
The note about showing all runs might also mean you want to literally see each run represented as a discrete point in your plot. For this... well, they already are! ggplot2 is plotting each of your points from your data into the chart. Since some points share the same values of x= and y=, you are getting overplotting - the points are drawn over top of one another.
If you want to visually see each point represented here, one option could be to use geom_jitter() instead of geom_point(). It's not really great here, because it will look like your data has different x and y values, but it is an option if this is what you want to do. Note in the code below I'm also changing the shape of the point to be a hollow circle for better clarity, where the color= is the line around each point (here it's black), and the fill= aesthetic is instead used for Volume. You should get the idea though.
set.seed(1234) # using the same randomization seed ensures you have the same jitter
ggplot(data = mydata, aes(x=Area, y=Time)) +
geom_jitter(aes(fill =as.factor(Volume), size = Run), shape=21, color='black') +
geom_line(aes(group=as.factor(Volume))) +
labs(
x = "Area", y = "Time", fill='Volume'
) +
scale_size_continuous(breaks=c(1:8)) +
theme_bw()
I have some diffraction data from XRD. I'd like to plot it all in one chart but stacked. Because the range of y is quite large, stacking is not so straight forward. there's a link to data if you wish to play and the simple script is below
https://www.dropbox.com/s/b9kyubzncwxge9j/xrd.csv?dl=0
library(dplyr)
library(ggplot2)
#load it up
xrd <- read.csv("xrd.csv")
#melt it
xrd.m = melt(xrd, id.var="Degrees_2_Theta")
# Reorder so factor levels are grouped together
xrd.m$variable = factor(xrd.m$variable,
levels=sort(unique(as.character(xrd.m$variable))))
names(xrd.m)[names(xrd.m) == "variable"] <- "Sample"
names(xrd.m)[names(xrd.m) == "Degrees_2_Theta"] <- "angle"
#colours use for nearly everything
cbPalette <- c("#000000", "#E69F00", "#56B4E9", "#009E73", "#F0E442", "#0072B2", "#D55E00", "#CC79A7")
#plot
ggplot(xrd.m, aes(angle, value, colour=Sample, group=Sample)) +
geom_line(position = "stack") +
scale_colour_manual(values=cbPalette) +
theme_linedraw() +
theme(legend.position = "none",
axis.text.y=element_blank(),
axis.ticks.y=element_blank()) +
labs(x="Degrees 2-theta", y="Intensity - stacked for clarity")
Here is the plot- as you can see it's not quite stacked
Here is something I had in excel a way back. ugly - but slightly better
I'm not sure that I will actually use the stacked plot function from R because I find it always looks off from past experience and instead might use the same data manipulation I used from excel.
It seems that you have a different understanding of the result of applying position="stack" on your geom_line() than what actually is happening. What you're looking to do is probably best served by either using faceting or creating a ridgeline plot. I will give you solutions for both of those approaches here with some example data (sorry, I don't click dropbox links and they will eventually break anyway).
What does position="stack" actually do?
The result of position="stack" will be that your y values of each line will be added, or "stacked", together in the resulting plot. That means that the lines as drawn will only actually accurately reflect the actual value in the data for one of the lines, and the other will be "added on top" of that (stacked). The behavior is best illustrated via an example:
ex <- data.frame(x=c(1,1,2,2,3,3), y=c(1,5,1,2,1,1), grp=rep(c('A','B'),3))
ggplot(ex, aes(x,y, color=grp)) + geom_line()
The y values for "A" are equal to 1 at all values of x. This is the same as indicating position="identity". Now, let's see what happens if we use position="stack":
ggplot(ex, aes(x,y, color=grp)) + geom_line(position="stack")
You should see, the value of y plotted for "B" is equal to B, whereas the y value for "A" is actually the value for "A" added to the value for "B". Hope that makes sense.
Faceting
What you're trying to do is take the overlapping lines you have and "separate" them vertically, right? That's not quite stacking, as you likely want to maintain their y values as position="identity" (the default). One way to do that quite easily is to use faceting, which creates what you could call "stacked plots" according to one or two variables in your dataset. In this case, I'm using example data (for reasons outlined above), but you can use this to understand how you want to arrange your own data.
set.seed(1919191)
df <- data.frame(
x=rep(1:100, 5),
y=c(rnorm(100,0,0.1), rnorm(100,0,0.2), rnorm(100,0,0.3), rnorm(100,0,0.4), rnorm(100,0,0.5)),
sample_name=c(rep('A',100), rep('B',100), rep('C',100), rep('D',100), rep('E',100)))
# plot code
p <- ggplot(df, aes(x,y, color=sample_name))
p + geom_line() + facet_grid(sample_name ~ .)
Create a Ridgeline Plot
The other way that kind of does the same thing is to create what is known as a ridgeline plot. You can do this via the package ggridges and here's an example using geom_ridgeline():
p + geom_ridgeline(
aes(y=sample_name, height=y),
fill=NA, scale=1, min_height=-Inf)
The idea here is to understand that geom_ridgeline() changes your y axis to be the grouping variable (so we actually have to redefine that in aes()), and the actual y value for each of those groups should be assigned to the height= aesthetic. If you have data that has negative y values (now height= values), you'll also want to set the min_height=, or it will cut them off at 0 by default. You can also change how much each of the groups are separated by playing with scale= (does not always change in the way you think it would, btw).
I want to create 3 graphs in ggplot2 as follows:
ggplot(observbest,aes(x=factor(iteration),y=bottles,colour=Team ,group=Team)) + geom_line() + scale_colour_gradientn(colours=rainbow(16))
ggplot(observmedium,aes(x=factor(iteration),y=bottles,colour=Team ,group=Team)) + geom_line() + scale_colour_gradientn(colours=rainbow(16))
ggplot(observweak,aes(x=factor(iteration),y=bottles,colour=Team ,group=Team)) + geom_line() + scale_colour_gradientn(colours=rainbow(16))
That is, three graphs displaying the same thing but for difference dataset each time. I want to compare between them, therefore I want their y axis to be fixed to the same scale with the same margins on all graphs, something the currently doesn't happen automatically.
Any suggestion?
Thanks
It sounds like a facet_wrap on all the observations, combined into a single dataframe, might be what you're looking for. E.g.
library(plyr)
library(ggplot2)
observ <- rbind(
mutate(observbest, category = "best"),
mutate(observmedium, category = "medium"),
mutate(observweak, category = "weak")
)
qplot(iteration, bottles, data = observ, geom = "line") + facet_wrap(~category)
Add + ylim(min_value,max_value) to each graph.
Another option would be to merge the three datasets with an id variable identifying which value is in which dataset, and then plot the three of them together, differentiating them by linetype for instance.
Use scale_y_continuous to define the y axis for each graph and make them all easily comparable.
In the data that I am attempting to plot, each sample belongs in one of several groups, that will be plotted on their own grids. I am plotting stacked bar plots for each sample that will be ordered in increasing number of sequences, which is an id attribute of each sample.
Currently, the plot (with some random data) looks like this:
(Since I don't have the required 10 rep for images, I am linking it here)
There are couple things I need to accomplish. And I don't know where to start.
I would like the bars not to be placed at its corresponding nseqs value, rather placed next to each other in ascending nseqs order.
I don't want each grid to have the same scale. Everything needs to fit snugly.
I have tried to set scales and size to for facet_grid to free_x, but this results in an unused argument error. I think this is related to the fact that I have not been able to get the scales library loaded properly (it keeps saying not available).
Code that deals with plotting:
ggfdata <- melt(fdata, id.var=c('group','nseqs','sample'))
p <- ggplot(ggfdata, aes(x=nseqs, y=value, fill = variable)) +
geom_bar(stat='identity') +
facet_grid(~group) +
scale_y_continuous() +
opts(title=paste('Taxonomic Distribution - grouped by',colnames(meta.frame)[i]))
Try this:
update.packages()
## I'm assuming your ggplot2 is out of date because you use opts()
## If the scales library is unavailable, you might need to update R
ggfdata <- melt(fdata, id.var=c('group','nseqs','sample'))
ggfdata$nseqs <- factor(ggfdata$nseqs)
## Making nseqs a factor will stop ggplot from treating it as a numeric,
## which sounds like what you want
p <- ggplot(ggfdata, aes(x=nseqs, y=value, fill = variable)) +
geom_bar(stat='identity') +
facet_wrap(~group, scales="free_x") + ## No need for facet_grid with only one variable
labs(title = paste('Taxonomic Distribution - grouped by',colnames(meta.frame)[i]))